Recombinant Rat NADPH oxidase 4 (Nox4)

Code CSB-CF015961RA(A4)
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Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
Nox4; Kox; NADPH oxidase 4; EC 1.6.3.-; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase
Rattus norvegicus (Rat)
Expression Region
Target Protein Sequence
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
Gene References into Functions
  1. chronic intermittent hypoxia-induced elevation of blood pressure is at least partially mediated via the Nox4-induced ROS/RhoA/ROCK pathway PMID: 28078487
  2. This study, for the first time, demonstrates that Nox4 is an oxygen-sensing enzyme and a main ROS source in NP cells. Nox4-dependent ROS are genotoxic and a potent trigger of NP cell senescence. Nox4 is a potential therapeutic target for disc cell senescence and IDD. PMID: 29147462
  3. The results demonstrate that the heightened sensitivity of the brain to ischemic damage is due to an organ-specific role of NOX4 in blood-brain barrier endothelial cells and neurons. PMID: 29087944
  4. Results indicate that intermedin exerts anti-oxidant effects by inhibition of Nox4, and the effect can be mediated by cAMP-PKA pathway in unilateral ureteral obstruction induced renal fibrosis. PMID: 28805491
  5. CYLD contributes to the transdifferentiation of adventitial fibroblasts via deubiquitinating Nox4 and may play a role in vascular remodeling. PMID: 28751569
  6. Oxygen-glucose deprivation/reoxygenation increased MEG3 and NOX4 expression in rat brain microvascular endothelial cells. PMID: 28634073
  7. increased mTAL luminal flow results in increases in intracellular and mitochondrial H2O2, which are dependent on the presence of Nox4, and that p67phox/Nox2 accounts solely for increases in O2 (.-) production PMID: 27279484
  8. Xbp1s plays an important role in NOX4-triggered cardiomyocyte hypertrophy via activating its downstream effector RIPK1, which may prove significant for the development of future therapeutic strategies. PMID: 27929749
  9. Nox4 activation in Vacular Smooth Muscle Cells contributes to actin depolymerization after hypoxia, which could be the underlying mechanism for myogenic tone impairment following ischemia/reperfusion. PMID: 27558234
  10. Upregulation of Nox4 is an important mechanism of cellular and mitochondrial oxidative stress leading to apoptotic cell death in cardiac myocytes following chronic beta- agonist stimulation. PMID: 26631573
  11. Luminal flow in thick ascending limbs leads to Nox4 recruitment to the nuclear region of thick ascending limb cells and an increase in O2 production. PMID: 27033446
  12. Acute restraint stress exacerbates the contractile hyper-reactivity in diabetic carotid arteries by enhancing Nox4 functionality. PMID: 26387612
  13. Hsp70 and CHIP mediates the ubiquitination and proteasomal degradation of Nox4 as part of the antioxidative effect of Losartan in spontaneous hypertensive rats. PMID: 26279425
  14. the reduced renal injury and attenuated blood pressure response to high salt in the SS(Nox4-/-) rat could be the result of multiple pathways by the knock out of Nox4. PMID: 26644237
  15. Glycated albumin increases the permissiveness of proximal tubular cells to fibrosis and apoptosis in vitro by triggering a pathway that involves Nox4. PMID: 25681565
  16. Results suggest that Nox4-mediated reactive oxygen species generation likely plays important role in fate determination and differentiation of neural crest stem cells. PMID: 25410908
  17. siNOX4 transfection markedly reduced rhBMP4-induced elevation of TRPC1 and 6 proteins. PMID: 25203114
  18. this study demonstrated a previously unrecognized protective role of Nox4, a ROS-generating enzyme and the major Nox isoform in CMECs, against H/R injury by inhibiting apoptosis and promoting migration and angiogenesis PMID: 24480752
  19. RhoA/ROCK is upstream of Poldip2-dependent Nox4 regulation and ROS production and induces redox signaling of kidney myofibroblast activation and may have broader implications in the pathophysiology of renal fibrosis. PMID: 24872317
  20. Nox4 is prominently expressed in the adventitia and contributes to altered fibroblast behavior, hypertensive vascular remodeling, and development of pulmonary hypertension. PMID: 24947524
  21. NOX4 activation is a decisive component in hepatic ischemia-reperfusion induced microcirculatory dysfunction. PMID: 24636100
  22. hyperglycemia could increase albumin permeability across the podocyte filtration barrier via Nox4-dependent PKGIalpha dimerization PMID: 24041960
  23. Data show that hypercholesterolemia affects myocardial microRNA expression, and by down-regulating microRNA-25 increases NOX4 expression and consequently oxidative/nitrative stress in the heart. PMID: 23722270
  24. Atrial fibroblasts show greater fibrotic and oxidative responses to TGF-beta1 than ventricular fibroblasts and suggest role for Nox4. PMID: 23884197
  25. Increased 8-OHdG concentration in the culture medium and higher expression of Nox-1, Nox-4, and p22(phox) mRNAs (3-6-fold) were observed in cells treated with AGE3. PMID: 23225244
  26. these results indicate that IL-1beta-dependent activation of PKCdelta is modulated by NOX4-derived ROS. PMID: 23022406
  27. the thyroid gland of females is exposed to higher oxidative stress levels due both to increased reactive oxygen species (ROS) production through NOX4, and decreased ROS degradation PMID: 23033809
  28. Nox4D is a nuclear-localized and functionally active splice variant of Nox4 that may have important pathophysiologic effects through modulation of nuclear signaling and DNA damage in vascular cells. PMID: 23393389
  29. Phenylephrine increased Nox4 protein expression and cardiomyocyte hypertrophy in a dose-dependent manner in cultured neonatal rat heart cells. PMID: 23271793
  30. Telmisartan downregulates myocardial expression of Nox4 in type 2 diabetic rats. PMID: 22873349
  31. NOX4 is the main catalytic isoform of NADPH oxidase that contributes to oxygen production in the thick ascending limb of kidney. PMID: 22875785
  32. Study shows that testosterone induces vascular smooth muscle cells (VSMCs) migration via NADPH oxidase (Nox1 and Nox4 mRNA levels and p47phox)-derived reactive oxygen species (ROS) and c-Src-dependent pathways. PMID: 22566500
  33. Abnormal expression of NADPH oxidase plays an important role in progression of hypertensive renal damage in spontaneously hypertensive rats. PMID: 21646815
  34. In the aorta, Nox4 expression was increased by three-fold in obese rats. PMID: 22195989
  35. This study provides strong evidence that Nox4 is an important source of reactive oxygen species (ROS) in the left ventricle and that Nox4-derived ROS contribute to cardiomyopathy at early stages of type 1 diabetes PMID: 22031600
  36. Intracellular reactive oxygen species formation partly through an increasing NOX4 activity in response to high pacing frequency is associated with an increased atrial natriuretic peptide secretion. PMID: 22063193
  37. IGF-I induces an increase in expression of Nox4 in cultured renal proximal tubular epithelial cells. PMID: 21940672
  38. Expression of Nox4 was increased in vascular smooth muscle cells from SHR. Nox4,alone or in association,may be involved in NAD(P)H oxidase-mediated ROS production. PMID: 21419746
  39. High NOX4 expression is associated with colon cancers. PMID: 20715105
  40. Our results indicate that miR-25 as an endogenous gene silencing factor importantly contributes to the regulation of NOX4 gene expression in diabetic nephropathy. PMID: 21071935
  41. Bilirubin and biliverdin protect rodents against diabetic nephropathy by downregulating NAD(P)H oxidase. PMID: 20686447
  42. Upregulation of Nox4 by hypertrophic stimuli and aging induces oxidative stress, apoptosis and left ventricular dysfunction, via mitochondrial insufficiency caused by increased O(2)(-) production and cysteine oxidation in mitochondrial proteins. PMID: 20185797
  43. Translocation of Hsp70 to proximal tubule membranes in SHRLos might exert a cytoprotective effect by down-regulation of NADPH subunits Nox4. PMID: 20016382
  44. NADPH oxidase-derived reactive oxygen species is one of critical components of a signal transduction pathway that links puromycin aminonucleoside nephrosis to TRPC6-mediated Ca(2+) signaling. PMID: 19910702
  45. Nox4-based NAD(P)H oxidase and generation of reactive oxygen species mediate the effect of ANG II on Akt/PKB activation and protein synthesis in glomerular mesangial cells PMID: 12842860
  46. Nox4 co-localizes with vinculin in focal adhesions in vascular smooth muscle cells. Its role may be correlated with its compartmentalization in focal adhesions. PMID: 14670934
  47. cardiac fibroblasts express Nox4/p22 phox-containing oxidant generating complex activated by H(2)O(2) PMID: 15848200
  48. role for Nox4 as the major source of ROS in the kidneys during early stages of diabetes PMID: 16135519
  49. Nox4-derived reactive oxygen species are critical to the maintenance of the differentiated phenotype of vascular smooth muscle cells. PMID: 17082491
  50. blood pressure and angiotensin II type 1 receptor activation are involved in the up-regulation of Nox1 and Nox4 expression, which could contribute to vascular injury during chronic hypertension PMID: 17283869

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Note=May localize to plasma membrane and focal adhesions.
Tissue Specificity
Expressed in vascular smooth muscle.
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