PTK2B Antibody

Datasheet
Code CSB-PA018994GA01HU
Size US$685
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Product Details

Uniprot No. Q14289
Target Names PTK2B
Alternative Names CADTK antibody; CAK-beta antibody; CAKB antibody; CAKbeta antibody; Calcium regulated non receptor proline rich tyrosine kinase antibody; Calcium-dependent tyrosine kinase antibody; Cell adhesion kinase beta antibody; E430023O05Rik antibody; EC 2.7.10.2 antibody; FADK 2 antibody; FADK2 antibody; FAK2 antibody; FAK2_HUMAN antibody; Focal adhesion kinase 2 antibody; MGC124628 antibody; PKB antibody; Proline-rich tyrosine kinase 2 antibody; Protein kinase B antibody; Protein Tyrosine Kinase 2 Beta antibody; Protein-tyrosine kinase 2-beta antibody; PTK antibody; PTK2B antibody; PTK2B protein tyrosine kinase 2 beta antibody; PYK2 antibody; RAFTK antibody; RAFTK2 antibody; Related adhesion focal tyrosine kinase antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Human PTK2B
Immunogen Species Homo sapiens (Human)
Isotype IgG
Purification Method Antigen Affinity purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.
Tested Applications ELISA,WB,IHC
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
Gene References into Functions
  1. LFA-1 cross-linking recruits and activates FAK1 and PYK2 to phosphorylate LAT selectively on a single Y-171 site that binds to the GRB2-SKAP1 complex and limits dwell times of T-cells with dendritic cells PMID: 28699640
  2. Pyk2 has a role in spine structure and synaptic function; its deficit contributes to Huntington's disease cognitive impairments PMID: 28555636
  3. Results provide evidence that Pyk2 phosphorylates STIM1 at its Y361 residue, activating thereby store-operated Ca(2+) entry. PMID: 28218251
  4. The authors' findings identify Pyk2 as a unique mediator of invadopodium formation and function and also provide a novel insight into the mechanisms by which Pyk2 mediates tumor cell invasion. PMID: 29133485
  5. Results show that VEGFA induces Pyk2 activation in mediating human retinal microvascular endothelial cell migration, sprouting and tube formation, and that Pyk2-mediated STAT3 activation is required for hypoxia-induced retinal neovascularization. PMID: 27210483
  6. Interestingly, rs2279590 locus has a widespread enhancer effect on two nearby genes, protein tyrosine kinase 2 beta (PTK2B) and epoxide hydrolase-2 (EPHX2); both of which have been previously associated with AD as risk factors. PMID: 28973302
  7. Multiple myeloma that is driven by deregulated iron homeostasis and/or Pyk2/beta-cateninn signaling is susceptible to deferasirox-induced apoptosis. PMID: 27602957
  8. In summary, our data suggested that PYK2 via S6K1 activation modulated AR function and growth properties in prostate cancer cells. Thus, PYK2 and S6K1 may potentially serve as therapeutic targets for PCa treatment. PMID: 27492635
  9. Our findings suggest that Pyk2 plays an important role in the coordination of stabilization of beta-catenin in the crosstalk between Wnt/beta-catenin and Wnt/Ca(2+) signaling pathways upon Wnt3a stimulation in differentiating hNPCs. PMID: 28694190
  10. STIM1-induced Ca(2+) signaling activates Pyk2 to inhibit the interaction of VE-PTP and VE-cadherin and hence increase endothelial permeability. PMID: 28385807
  11. Ascites and CCL18 stimulate the phosphorylation and expression of Pyk2, which positively regulates ascites-induced ovarian cancer cell migration. PMID: 27613122
  12. We demonstrated trophoblast cytoprotection by intervention with supraphysiological concentrations of relaxin, a process in part mediated through the PI3-kinase-Akt/PKB cell survival pathway. These results provide further rationale for clinical investigation of relaxin as a potential therapeutic in preeclampsia. PMID: 28122716
  13. PTK2B polymorphism (rs28834970) could modify the risk of late-onset Alzheimer's disease (LOAD), and PTK2B polymorphism (rs28834970) and APOE may interact to increase LOAD risk in a Han Chinese population. PMID: 27080426
  14. Studies suggest that PYK2 is a common downstream effector of ErbB and IL8 receptors, and that PYK2 integrates their signaling pathways through a positive feedback loop to potentiate breast cancer invasion. PMID: 26084289
  15. Pyk2 is a key downstream signaling molecules of CCR7 in SCCHN, which promotes SCCHN tumorigenesis and progression. PMID: 26352169
  16. Phosphoproteomic analysis identifies FAK2 as a potential therapeutic target for tamoxifen resistance in breast cancer. PMID: 26330541
  17. Pyk2-focal adhesion targeting domain interacts with and binds to leupaxin. PMID: 26866573
  18. Src has a role in priming Pyk2 (but not FAK) phosphorylation and subsequent activation downstream of integrins PMID: 26866924
  19. Data strongly suggest that chemokine-stimulated associations between Vav1, SLP-76, and ADAP facilitate Rac1 activation and alpha4beta1-mediated adhesion, whereas Pyk2 opposes this adhesion by limiting Rac1 activation. PMID: 26202465
  20. FAK and PYK2 functioned redundantly to promote the Wnt/beta-catenin pathway by phosphorylating GSK3beta(Y216) to reinforce pathway output-beta-catenin accumulation and intestinal tumorigenesis. PMID: 26274564
  21. This study identified Pyk2 as a cellular component required for the intracellular trafficking of HPV16 during infection. PMID: 26109718
  22. These results suggest that melatonin exerts anti-migratory and anti-invasive effects on glioma cells in response to hypoxia via reactive oxygen species-alphavbeta3 integrin-FAK/Pyk2 signaling pathways. PMID: 25889845
  23. data suggest a novel role for FAK in GPVI-dependent ROS formation and platelet activation and elucidate a proximal signaling role for FAK within the GPVI pathway. PMID: 25415317
  24. Orai1/Pyk2 pathway is essential for glioma migration and invasion. PMID: 25433371
  25. Pyk2-NDRG1 axis is possibly involved in conveying the anti-proliferative effect of beta-ionone in prostate cancer cells. PMID: 25219547
  26. Pyk2 has a role in integrin regulation of size and dynamics of signaling microclusters PMID: 25778396
  27. PYK2 is an intermediary component of Ca(2+) signaling between PKA-mediated and Tyr phosphorylations that is required for achieving functional human sperm capacitation. PMID: 25180269
  28. Data indicate that chicken paxillin leucine-aspartate LD2 and LD4 motifs preferentially dock at the helix 2/3 binding site of human Proline-rich tyrosine kinase 2 (Pyk2)-focal adhesion kinase (FAK). PMID: 25174335
  29. these data demonstrate that Pyk2 is a critical regulator of PI3K function downstream of the TCR. PMID: 25387834
  30. Data indicate the tumor-promoting role of proline-rich tyrosine kinase 2 (Pyk2) in multiple myeloma (MM), suggesting tyrosine kinase inhibitor as a therapeutic option in MM. PMID: 25217697
  31. inhibition of FAK, PYK2 and BCL-XL synergistically enhances apoptosis in ovarian clear cell carcinoma cell lines PMID: 24523919
  32. Pyk2 and Src are important in CCL18-induced breast cancer metastasis. PMID: 24142406
  33. High expression of proline-rich tyrosine kinase 2 is associated with hepatocellular carcinoma via regulating phosphatidylinositol 3-kinase/AKT pathway. PMID: 22618716
  34. Development of a coordinated allo T cell and auto B cell response against autosomal PTK2B after allogeneic hematopoietic stem cell transplantation. PMID: 24097630
  35. Pyk2 is essential for skin wound reepithelialization in vivo and in vitro and that it regulates epidermal keratinocyte migration via a pathway that requires PKCdelta and MMP functions. PMID: 24598361
  36. Pyk2 is a shared key mediator of targeted-therapy induced adhesion and migration; Targeting Pyk2 may serve as an effective therapeutic strategy to reduce extramedullar relpase in acute promyelocytic leukemia and chronic myeloid leukemia. PMID: 24176282
  37. We hypothesize a potential direct or indirect role for SRC, RAF1, PTK2B genes in neurotransmission and in central nervous system signaling processes. PMID: 24108181
  38. Determine a crucial role of LRP1-mediated Pyk2 phosphorylation on hypoxia-induced MMP-9 activation and hVSMC migration and therefore in hypoxia-induced vascular remodeling. PMID: 24072693
  39. Both Pyk2 and phosphorylated Pyk2[pY881] are potential prognostic factors and therapeutic targets for non-small-cell lung cancer. PMID: 23922106
  40. Our results confirm that RTKs are frequently altered in chordomas. PMID: 23618355
  41. Results indicate that in vitro Pyk2 might function to regulate cell adhesion and motility following all-trans-retinoic acid (ATRA). PMID: 23587524
  42. We conclude that the effects of selenoprotein H on mitochondrial biogenesis and mitochondrial function are probably mediated through protein kinase A-CREB-PGC-1alpha and Akt/protein kinase B-CREB-PGC-1alpha pathways. PMID: 23220172
  43. Data indicate protein secretion pathways activated by monosodium urate (MSU) in macrophages, and reveal a novel role for cathepsin B and Src, Pyk2, PI3 kinases in the activation of unconventional protein secretion. PMID: 23292187
  44. These results identify a novel pathway of integrin alphaIIbbeta3 outside-in signaling and recognize the tyrosine kinase Pyk2 as a major regulator of platelet adhesion and spreading on fibrinogen. PMID: 23216754
  45. the non-catalytic functions of the kinases Fyn and Pyk2 were required for late stage human T cell adhesion. PMID: 23300847
  46. SOCS3 definitely plays roles in regulating Pyk2 signaling and cell motility in A549 cells. PMID: 23302305
  47. PAC1 regulates PYK-2 tyrosine phosphorylation in a calcium-dependent manner in lung cancer cell lines. PMID: 22581436
  48. findings showed that Pyk2 is overexpressed in squamous cell carcinoma of the head and neck; data suggest that CCR7 via Pyk2 and cofilin regulates the chemotaxis and migration ability of metastatic squamous cell carcinoma of the head and neck cells PMID: 22923218
  49. Down-regulation of both miR-517a and miR-517c contribute to hepatocellular carcinoma cells development through regulating Pyk2. PMID: 23142219
  50. Pyk2, which was strongly activated by IGF-I, was critical for IGF-IR-dependent motility. PMID: 22859931
  51. bFGF significantly stimulated PKB activity in CNE- I nasopharyngeal carcinoma cell line. PMID: 15835820
  52. Pyk2 as a critical regulator of endothelial cell inflammation by virtue of engaging IKK to promote the release and the transcriptional capacity of RelA/p65. PMID: 22842493
  53. Pyk2 plays a crucial role in G protein-coupled receptor agonist thrombin-induced human aortic smooth muscle cell growth and migration, as well as balloon injury-induced neointima formation. PMID: 22922962
  54. duced expression of miR-23b enhances glioma cell migration in vitro and invasion ex vivo via modulation of Pyk2 protein expression. PMID: 22745829
  55. Report safety, pharmacokinetic, and pharmacodynamic phase I dose-escalation trial of PF-00562271, an inhibitor of focal adhesion kinases, in advanced solid tumors. PMID: 22454420
  56. Data show that c-Met induces FAK and Pyk2 phosphorylation in medulloblastoma cells. PMID: 22188814
  57. This is a review of recent developments in Protein kinase B/AKT and focal adhesion kinase signaling in cancer with the particular emphasis on the novel signaling pathways in which FAK is downstream of AKT. [review] PMID: 22023045
  58. Results may suggest a new evidence of Pyk2 on promoting cisplatin resistance of HCC cells through preventing cell apoptosis, activation of AKT pathway and upregulation of drug resistant genes. PMID: 22096562
  59. ATP stimulates epithelial cell motility through Pyk2-mediated activation of the EGF receptor PMID: 21840393
  60. although staurosporine-mediated caspase activation and FAK cleavage was delayed in the mtp53-R 175H HOSCC cell line, caspase-8 activation and FAK dephosphorylation were induced by increasing the apoptotic stimulus. PMID: 21426305
  61. Activation of Pyk2 in the heart during heart failure may contribute to protection against arrhythmia. PMID: 21666110
  62. downregulation of endogenous FIP200 protein in glioblastoma tumor cells, astrocytes, and brain microvessel endothelial cells promotes apoptosis, most likely due to the removal of a direct interaction of FIP200 with Pyk2 that inhibits Pyk2 activation PMID: 21602932
  63. data provided new evidence of the underlying mechanism of Pyk2 in controlling cell motility of HCC cells through regulation of genes associated with EMT PMID: 21533080
  64. The results suggest a surprising finding that limited cell adhesion can enhance endothelial responsiveness to VEGF and demonstrate a novel role for Pyk2 in the adhesive regulation of angiogenesis. PMID: 21640103
  65. Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of nephrocystin-1 to control targeting to monocilia PMID: 21357692
  66. Both wild-type and internal tandem duplication (ITD)-FLT3 proteins co-immunoprecipitated with beta1 integrin and Pyk2 indicating the signal crosstalk between FLT3, beta1 integrin and Pyk2. PMID: 21114537
  67. Phosphorylation of focal adhesion kinase at Tyr397 is associated with gastric carcinomas. PMID: 20724588
  68. Data reveal that Pyk2 couples the activation mGluRs to the ERK1/2 mitogen-activated protein kinase pathway even though it attenuates mGluR1-dependent G protein signaling. PMID: 20180987
  69. TNFRSF19 overexpression in glioma cells activates Rac1 signaling in a Pyk2-dependent manner to drive glioma cell invasion and migration PMID: 20881009
  70. The Pyk2 FERM domain is involved in the regulation of Pyk2 activity by acting to regulate the formation of Pyk2 oligomers that are critical for Pyk2 activity. PMID: 20849950
  71. Studies suggest that FAK FERM domains might mediate information transfer between the cell cortex and nucleus. PMID: 20966971
  72. Pyk2 acts as an early sensor of numerous extracellular signals that trigger a Ca(2+) flux and/or reactive oxygen species to amplify tyrosine phosphorylation signaling events. PMID: 20688918
  73. Data show that knockdown of SOCS2 resulted in the accumulation of p-Pyk2(Tyr402) and blocked NK cell effector functions. PMID: 20543098
  74. Pyk2 and cAMP interact in regulating prostate cell functions and in "keeping" prostate identity. PMID: 19106639
  75. Data show that Pyk2 had a second burst of phosphorylation 30-60 min after TCR stimulation. PMID: 20381867
  76. The data demonstrated that Pyk2 and PECAM-1 were critical mediators of both anchorage-independent growth and anoikis resistance in tumor cells. PMID: 20074345
  77. activation of Pyk2 is an early signal that promotes wound healing by stimulating the SFK/EGFR signaling pathway. PMID: 20215112
  78. Studies indicated that the roles of FAK in tumorigenesis are different among the tumor species. PMID: 19458494
  79. TCR has a distinct pathway for the activation of Pyk2 compared with other receptor systems. PMID: 20028775
  80. The p110alpha selectively mediates activation of the Tyr kinase Pyk2 to regulate barrier function. PMID: 20308428
  81. Upregulation of serum proline-rich tyrosine kinase 2 is associated with systemic lupus erythematosus. PMID: 19930834
  82. Pyk2 functions to limit p53 levels, thus facilitating cell growth and survival in a kinase-independent manner. PMID: 19880522
  83. Glomerular crescents strongly expressed cell adhesion kinase beta. PMID: 11774117
  84. Human umbilical vein endothelial cells express mRNA transcripts for both the full length isoform Pyk2 and the truncated isoform Pyk2-H containing the C-terminal deletion. PMID: 11820787
  85. Suppressees androgen receptor transactivation vir interaction and phosphorylation of ARA44 coregulator PMID: 11856738
  86. Pyk2 role in platelet activation by vWF in the early signal transduction events activated by ligand binding to glycoprotein Ib-IX-V. PMID: 11916084
  87. Transforming growth factor-beta 2 is a transcriptional target via forkhead transcription factor PMID: 12011061
  88. By rapidly translocating to the vicinity of the immune synapse after T cell receptor stimulation, Pyk2 plays an essential role in T cell activation and polarized secretion of cytokines. PMID: 12077257
  89. This protein binds to periplakin. PMID: 12244133
  90. role in negative regulation of mixed lineage kinase 3 results in cell survival PMID: 12458207
  91. Critical role of the carboxyl terminus of proline-rich tyrosine kinase (Pyk2) in the activation of human neutrophils by tumor necrosis factor: separation of signals for the respiratory burst and degranulation. PMID: 12515814
  92. results indicate that signaling via protein kinase B to forkhead transcription factor FKHR can account for the effect of insulin to regulate peroxisome proliferator-activated receptor-gamma coactivator-1 promoter activity via the insulin response sequence PMID: 12606503
  93. PYK2 regulates transendothelial migration of cultured NK cells in response to chemokines by controlling the activation of the small GTP binding protein Rac, and thus acts as an integration point between integrin and chemokine receptor stimulation. PMID: 12626562
  94. Augmented activation of Pyk2 is an immediate signaling event required for the trans-regulation of integrin alpha L beta 2 by alpha 4 beta 1 in Jurkat T cells. PMID: 12794117
  95. RAFTK is activated by VEGF in human brain microvascular endothelial cells PMID: 12844492
  96. PKB is an essential component of the FSH-mediated granulosa cell differentiation and that both PKB and G(s)alpha signaling pathways are required. PMID: 12933673
  97. results indicate that activation of PKC is responsible for GnRH-induced phosphorylation of both ERK1/2 and Pyk2, and that Pyk2 activation does not contribute to GnRH signaling PMID: 12943720
  98. A novel regulatory network RAFTK/Pyk2, Src and p38 appears to be critical for VEGF-induced endothelial cell migration. PMID: 14676843
  99. Calcium signaling in ovarian surface epithelial cells not only induces telomerase activity via JNK but also activates Pyk2. PMID: 14729602
  100. identified a BCR/ABL-dependent increase in expression of multiple genes involved in pre-mRNA splicing; alternative splicing of gene PYK2 resulted in increased expression of full-length Pyk2 in BCR/ABL-containing cells PMID: 14961028

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Involvement in disease Aberrant PTK2B/PYK2 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. Elevated PTK2B/PYK2 expression is seen in gliomas, hepatocellular carcinoma, lung cancer and breast cancer.
Subcellular Location Cytoplasm, Cytoplasm, perinuclear region, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cell junction, focal adhesion, Cell projection, lamellipodium, Cytoplasm, cell cortex, Nucleus
Protein Families Protein kinase superfamily, Tyr protein kinase family, FAK subfamily
Tissue Specificity Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.
Database Links

HGNC: 9612

OMIM: 601212

KEGG: hsa:2185

STRING: 9606.ENSP00000332816

UniGene: Hs.491322

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