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Recombinant Bacillus subtilis Chromosome partition protein Smc (smc), partial

Recombinant Bacillus subtilis Chromosome partition protein Smc (smc), partial

Code	CSB-YP347277BRJ
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP347277BRJ
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP347277BRJ-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP347277BRJ
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP347277BRJ
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

smc

Uniprot No.

P51834

Alternative Names

smc; ylqA; BSU15940; Chromosome partition protein Smc

Species

Bacillus subtilis (strain 168)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Required for chromosome condensation and partitioning.

Gene References into Functions

Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. This mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms. PMID: 28689660
we show that rapid inactivation of SMC or its partner protein ScpB during fast growth leads to a failure to resolve newly replicated origins and a complete block to chromosome segregation. PMID: 24440393
A mutation in SMC that affects ATPase activity and results in exaggerated DNA binding causes a strong segregation defect and affects the localization of the SMC complex, which localizes to many more sites in the cell than under normal conditions PMID: 23475963
An Smc dimer is bridged by a single ScpAB to generate asymmetric tripartite rings analogous to eukaryotic SMC complexes. PMID: 23353789
has a dual function, in chromosome supercoiling and in active segregation PMID: 16272394

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Subcellular Location

Cytoplasm. Note=Probably associated with the nucleoid.

Protein Families

SMC family

Database Links

KEGG: bsu:BSU15940

STRING: 224308.Bsubs1_010100008791

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