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The N-terminal 6xHis-SUMO-tagged recombinant human HLA-E is expressed in E.coli. Its expression region maps within amino acids 22-305 (extracellular domain) of the human HLA-E. Its purity is greater than 90% measured by SDS-PAGE. It migrated to the molecular weight band of approximately 50 kDa on the gel. This recombinant human HLA-E protein is available now. The target protein HLA-E exerts a very specialized role in cell recognition by NK cells.
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1.Is the 6xHis-SUMO tag retained or is it cleaved after your expression?
2.Has this molecule been shown to bind to anti-HLA-E and anti-HLA-ABC (pan class I) antibodies?
I hope this email finds you well. I am interested in this protein but they would like to know more information regarding its biological activity. Is this information available for me to share?