Recombinant Human Heat shock cognate 71 kDa protein(HSPA8)

Code CSB-EP010829HU
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Source E.coli
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Code CSB-EP010829HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010829HU
Size Pls inquire other sizes
Source Baculovirus
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Code CSB-MP010829HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names HSPA8
Uniprot No. P11142
Alternative Names 2410008N15Rik; Constitutive heat shock protein 70; Epididymis luminal protein 33; Epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 8; Heat shock 70kD protein 10; Heat shock 70kD protein 8; Heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; Heat shock cognate protein 54; Heat shock cognate protein 71 kDa; Heat shock protein 8; Heat shock protein A8; Heat shock protein family A (Hsp70) member 8; Heat-shock70-KD protein 10, formerly; HEL 33; HEL S 72p; HSC54; HSC71; Hsc73; HSP71; HSP73; HSP7C_HUMAN; HSPA10; HSPA8; LAP1; Lipopolysaccharide associated protein 1; LPS associated protein 1; LPS associated protein; MGC102007; MGC106514; MGC114311; MGC118485; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; NIP71
Species Homo sapiens (Human)
Expression Region 2-646
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation
Gene References into Functions
  1. Data suggest that both the small heat shock protein HspB1/Hsp27 and the constitutive chaperone Hsc70/HspA8 interact with tau to prevent tau-fibril/amyloid formation. Hsc70 is highly efficient at preventing tau-fibril elongation, possibly by capping ends of tau-fibrils. (HspB1 = heat shock protein family B small member 1; Hsc70 = heat shock protein family A Hsp70) PMID: 29298892
  2. This study demonstrated that particular distribution for HSC70 and PSMC4 in the cytoplasm and accumulation within Lewy body in the dopaminergic neurons of the substantia nigra in Parkinson patients. PMID: 29218503
  3. Hsp70participated in PINK1-mediated mitophagy by stabilizing PINK1. PMID: 29107085
  4. These results demonstrate not only an important mechanism of Hsc70 in facilitating EV-A71 replication, but also a target for antiviral drug development. PMID: 29180285
  5. Post-transcriptional inhibition of HSPA8 expression leads to synaptic vesicle cycling defects in multiple models of amyotrophic lateral sclerosis. PMID: 28978466
  6. Taken together, these data suggest hat the altered hydrogen bonding observed in the Hsc70 C17W mutant (where the connectivity between Mg2+.nucleotide and E175 is also disrupted) could bring about changes to Hsc70 domain communication, affecting peptide association while also limiting ATP hydrolysis. PMID: 29300467
  7. The roles of the E3 ubiquitin ligase, carboxy-terminus Hsc70 interacting protein (CHIP) in various types of cancers. PMID: 28731191
  8. Hsc70/Hsp90 chaperones contribute to the conformational and functional maintenance of DeltaF508-CFTR at 37 degrees C. PMID: 28855508
  9. Engagement of the oligomer by LAP1 triggers ATP hydrolysis and rapid complex disassembly. Thus the Torsin complex is a highly dynamic assembly whose oligomeric state is tightly controlled by distinctively localized cellular cofactors. PMID: 28814508
  10. Downregulation of Hsc70, Hsp70, and IL-15 expression at gene and/or protein levels might support the retention of fertilization products in cases of missed abortion and blighted ovum. PMID: 27225940
  11. STRO-1 binds to immune-precipitated HSC70 and siRNA-mediated knock down of HSPA8 reduced STRO-1 binding. PMID: 28026090
  12. Synapsin is part of a multiprotein complex enriched in chaperones/cochaperones including Hsc70. Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin. PMID: 28559423
  13. study demonstrates a critical role of Hsc70 in SV40 endoplasmic reticulum-to-cytosol penetration and reveal how SGTA controls Hsc70 to impact this process PMID: 28356524
  14. In fact, DnaJC5 overexpression induced tau release in cells, neurons, and brain tissue, but only when activity of the chaperone Hsc70 was intact and when tau was able to associate with this chaperone. PMID: 27261198
  15. These results suggest that Bag1 and Bag3 control the stability of the Hsc70-client complex using at least two distinct protein-protein contacts, providing a previously under-appreciated layer of molecular regulation in the human Hsc70 system. PMID: 27474739
  16. While cerebrospinal fluid Nrf2 and HSPA8 do not appear to offer diagnostic biomarkers for Parkinson's disease (PD), the associations between Nrf2 levels and UPDRS scores in LRRK2 + PD patients merit further investigation PMID: 26526034
  17. HSPA8 maintains pluripotency of human pluripotent stem cells by binding to the master pluripotency regulator OCT4 and facilitating its DNA-binding activity. PMID: 26549849
  18. heat shock cognate 70 (HSC70) is an essential component of Aes foci in colorectal cancer cells. PMID: 26229111
  19. Cx43-Hsc70 interaction regulates cell cycle G1/S progression through a novel mechanism by which Cx43-Hsc70 interaction prevents the nuclear accumulation of p27 through controlling the nuclear translocation of cyclin D1-CDK4-p27 complex. PMID: 26481195
  20. serum HSC71 was identified as a novel serum biomarker of renal cell carcinoma, particularly useful in early diagnosis of clear-cell type. PMID: 26425554
  21. These findings provide further evidence that histatin 3 may be involved in the regulation of cell proliferation, particularly during G1/S transition, via the ubiquitin-proteasome system of p27(Kip1) and HSC70. PMID: 26775844
  22. constitutive heat shock protein HSC70 forms granule-like structures in the cytoplasm of human cells several days after the exposure to heat stress PMID: 26335814
  23. These results indicate that CHIP decreases the Kv1.5 protein level and functional channel by facilitating its degradation in concert with chaperone Hsc70 PMID: 26232501
  24. Active Hsc70 requires active tau to regulate microtubule assembly in vivo, suggesting that tau acts in some ways as a co-chaperone for Hsc70 to coordinate microtubule assembly. PMID: 25882706
  25. detailed and systematic investigation to characterize if there are significant differences in the CHIP in vitro ubiquitination of human Hsp70 and Hsc70. PMID: 26010904
  26. HSP70 binds CD91 and TLR4 on decidual CD1a(+) dendritic cells, causes their maturation, and increases IL-15 in the context of Th1 cytokine/chemokine domination, which could support immune response harmful for ongoing pregnancy. PMID: 25737151
  27. LAP1 mutations are associated with severe dystonia, cerebellar atrophy and cardiomyopathy. PMID: 26596547
  28. HSC70 may serve as a molecular switch to modulate endocytic and autophagy pathways, impacting the source of antigens delivered for MHC class II presentation during cellular stress. (Review) PMID: 25953005
  29. CHIP docks onto Hsp70/Hsc70 and defines a bipartite mode of interaction between TPR domains and their binding partners. PMID: 25684577
  30. Hsc70 interacted with Rab1A in a chaperone-dependent mannerand Hsc70 knockdown decreased the level of Rab1A and increased the level of its ubiquitination under stress conditions. PMID: 24801886
  31. Data suggest that nhibition of nestin and heat shock protein HSPA8/HSC71 may be a useful molecular target in the treatment of glioblastomas. PMID: 25527454
  32. LAP1 and LULL1 regulate Torsin ATPase activity through an active site complementation mechanism. PMID: 25352667
  33. H-ERG trafficking was impaired by H2O2 after 48 h treatment, accompanied by reciprocal changes of expression between miR-17-5p seed miRNAs and several chaperones (Hsp70, Hsc70, CANX, and Golga20) PMID: 24386440
  34. the endogenous DNAJC12 and Hsc70 proteins interact in LNCaP cells. PMID: 24122553
  35. Endoplasmic reticulum-associated degradation of Niemann-Pick C1: evidence for the role of heat shock proteins and identification of lysine residues that accept ubiquitin. PMID: 24891511
  36. Results identify a systemic hsc70 reduction in Parkinson's disease patients. PMID: 24361989
  37. Hsp73 sustains Hsp90 chaperone function and critically contributes to cell survival in multiple myeloma patients. PMID: 23065523
  38. These findings indicate that wild type ILK and the non-oncogenic ILK(R211A) mutation comprise a cardioprotective module with Hsp/c70. PMID: 24260102
  39. A significant downregulation of HSPA8 and HSPA9 was observed in AD across the three brain regions compared to the controls, suggesting their participation in AD pathogenesis. PMID: 23948933
  40. HSP8A polymorphism is associated with drug-naive schizophrenia. PMID: 24548631
  41. Cx43-Hsc70 interaction probably plays a critical role during G1/S progression. PMID: 24056538
  42. These results demonstrate that Hsp70 recruits PP5 and activates its phosphatase activity which suggests dual roles for PP5 that might link chaperone systems with signaling pathways in cancer and development. PMID: 24327656
  43. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis. PMID: 23539416
  44. the U-box mutation stimulated CHIP binding to Hsc70 while promoting CHIP oligomerization. CHIP binding to Hsc70 binding was also stimulated by the presence of an Hsc70 client with a preference for the ADP-bound state. PMID: 23990462
  45. GLIPR1 interacts with Hsc70, and GLIPR1 overexpression or Hsc70 knockdown leads to transcriptional suppression of AURKA and TPX2. PMID: 23333597
  46. PKCiota knockdown sensitized cells to oxidative stress-induced apoptosis, whereas forced PKCiota expression counteracted the oxidative stress-induced apoptosis via Hsc70. PMID: 23224638
  47. Relaxation-based NMR experiments on the Hsc70-CHIP complex determined that the two partners move independently in solution, similar to "beads on a string PMID: 23865999
  48. LAP1 and LULL1 as regulatory cofactors that are responsible for the activation of TorA's ATPase activity. PMID: 23569223
  49. 70-kDa heat shock cognate protein hsc70 mediates calmodulin-dependent nuclear import of the sex-determining factor SRY. PMID: 23235156
  50. Studies suggest that Hsc70 and lysosome-associated protein 2A (LAMP-2A) through chaperone-mediated autophagy (CMA) play a role in the clearance of Htt and suggest a novel strategy to target the degradation of mutant huntingtin (Htt). PMID: 23071649
  51. thise preliminary result indicated that HSP70 was related to the phosphorylation of MK2, a specific nuclear downstream target of p38, suggesting HSP70 is a potential chaperone for the nuclear translocation of p38. PMID: 22842575
  52. the client binding domain of Hsc70 and Ssa1p binds two regions within alpha-Syn similar to a tweezer, with the first spanning residues 10-45 and the second spanning residues 97-102. PMID: 22843682
  53. Hsc70 is a prerequisite for the surface translocation and angiogenic function of nucleolin, which suggests strategies to target both Hsc70 and NCL for more effective antiangiogenic therapies. PMID: 22743058
  54. downregulation of HSC70 resulted in increased levels of DeltaF508-CFTR complexes with the co-chaperone BAG3 that in addition appeared to co-localize with the mutated protein on the cell surface. PMID: 22170045
  55. It is proposed that Az suppresses ubiquitination of DeltaF508-CFTR maybe by blocking interaction of the mutant with Hsc70 and CHIP, and, as a consequence, it enhances membrane trafficking of the mutant PMID: 22074182
  56. Partially unfolded states of AGXT strongly interact with Hsc70 and Hsp90 chaperones. PMID: 21103899
  57. Hsc70 is recruited to reovirus viral factories independently of its chaperone function. PMID: 22090113
  58. Addition of Hsp70 to a mixture of oligomeric and fibrillar tau aggregates prevents the toxic effect of these tau species on fast axonal transport. PMID: 22039833
  59. The ability of the cytosolic cognate 70-kDa heat-shock protein (Hsc70) chaperone system to modulate cell surface expression of MC4R, was investigated. PMID: 21719532
  60. Apoptin induced the translocation of endogenous Hsc70 from the cytoplasm to the nucleus, and both were co-localized in the nucleus. PMID: 21565169
  61. JB12 cooperates with cytosolic Hsc70 and the ubiquitin ligase RMA1 to target CFTR and CFTRDeltaF508 for degradation. PMID: 21148293
  62. The importance of Hsc70 in AtlE adhesin-mediated internalization was demonstrated by the inhibition of Staphylococcus aureus internalization with anti-Hsc70 antibodies. PMID: 20642807
  63. Immunocytochemical staining using this newly established antibody revealed that Hsc70 localizes predominantly in the cytoplasm in unstressed cells, whereas oxidative stress produced by H2O2 induces Hsc70 to translocate into the nucleus. PMID: 21050049
  64. genetic variants in HSPA8 gene (especially promoter SNP rs2236659) contribute to the coronary heart disease susceptibility by affecting its expression level PMID: 20300519
  65. the C331A mutant of neuronal nitric-oxide synthase is labilized for Hsp70/CHIP (C terminus of HSC70-interacting protein)-dependent ubiquitination PMID: 20729196
  66. Heteromeric complexes of Hsp70B', Hsp70, and Hsc70 were detected in differentiated human SH-SY5Y neuronal cells. PMID: 20084477
  67. This is the first work reporting the localization of the GlcNAc-binding domain of a member of the HSP70 family. PMID: 20804732
  68. PI 3-kinase and MEK kinase signaling as well as tyrosine dephosphorylation are essential for the accumulation of hsc70 in nucleoli of stressed cells PMID: 20457599
  69. suggesting that HSP70 overexpression may regulate lipopolysaccharide-induced cytokines expression through NF-kappaB pathway. PMID: 19551494
  70. Heat shock protein 70 (Hsc70) plays a significant role in vascular endothelial cells via the phosphatidylinositol 3-kinase/Akt pathway. PMID: 20018937
  71. Results identified heat shock protein 70 (Hsp70) that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with mild cognitive impairment. PMID: 19686046
  72. surface located Hsc70 on trophoblast giant cells mediates the uptake of pathogenic bacteria and proteins containing the TPR domain inhibit the function of Hsc70 by binding to its EEVD motif PMID: 20003465
  73. Data show that Hsc70 appears active in breast cancer cells and hypersecreted by direct cath D inhibition. PMID: 19802014
  74. Tumor-derived HSP70 peptide complexes have the immunogenic potential to instruct dendritic cells to cross-present endogenously expressed, nonmutated, and tumor antigenic peptides shared among tumors of the melanocytic lineage for T cell recognition. PMID: 12421917
  75. Hsc70 may be involved in androgen action on dermal papilla cells. PMID: 12850307
  76. no abnormal levels of hsc70 and hsp60 were detectable in pediatric c-ALL pre B-cells at diagnosis nor at relapse. In contrast, developmentally regulated levels of hsc70 and hsp60 expression during B-cell ontogenesis were observed PMID: 12890148
  77. tau binds to Hsc70, and its phosphorylation is a recognition requirement for the addition of ubiquitin PMID: 14612456
  78. a novel nuclear export and nuclear localization-related signals in human Hsp70 PMID: 14684748
  79. a region of the TfR that can potentially interact with hsc70 PMID: 15086793
  80. sequestered in discrete foci in the nucleus of the infected cell in herpes simplex virus type 1 infection. PMID: 15194794
  81. Hsp105alpha is suggested to regulate the substrate binding cycle of Hsp70/Hsc70 by inhibiting the ATPase activity of Hsp70/Hsc70, thereby functioning as a negative regulator of the Hsp70/Hsc70 chaperone system. PMID: 15292236
  82. Expressed in colon cancer cells [NIP71] PMID: 15336565
  83. The head shock protein 70 kD (HSP-70i) has been shown to protect cells, tissues, and organs from harmful assaults in vivo and in vitro experimental models. PMID: 15625011
  84. In this study, we discovered that the U-box ubiquitin ligase carboxyl-terminus of Hsc70 interacting protein (CHIP) ubiquitylated Epsin. PMID: 15694383
  85. Present in cells as part of the dengue virus receptor. PMID: 15795242
  86. CSP modulates G protein function by preferentially targeting the inactive GDP-bound form of G alpha(s) and promoting GDP/GTP exchange; the guanine nucleotide exchange activity of full-length CSP is regulated by Hsc70-SGT PMID: 15972823
  87. This study identified heat shock protein 70 protein 8 as being expressed on the cell surface and downregulated upon differentiation. PMID: 16100000
  88. Histidine 89 is an essential residue for Hsp70 in the phosphate transfer reaction. PMID: 16817320
  89. Hsc70 is directly associated with Influenza virus matrix protein 1 and therefore is required for viral production PMID: 17022977
  90. Hsc70 plays a defined role in reovirus outer capsid disassembly, during or soon after membrane penetration, to prepare the entering particle for gene expression and replication PMID: 17284448
  91. serum Hsp70 concentrations are decreased in normal human pregnancy PMID: 17296233
  92. Following co-immunoprecipitation, a specific interaction between the head domain of K5 with Hsc70, a chaperone also involved in vesicle uncoating, was verified. PMID: 17397964
  93. FKBP38 is a co-chaperone of HERG and contributes via the Hsc70/Hsp90 chaperone system to the trafficking of wild type and mutant HERG potassium channels PMID: 17569659
  94. 70-kDa heat shock proteins as a AQP2 interactors and have shown for hsc70 that this interaction is involved in AQP2 trafficking. PMID: 17636261
  95. Important functions of recently activated dendritic cells are thus critically modulated by the newly discovered HSPA8-EWI-2 interaction. PMID: 17785435
  96. Amino acid domains 280-297 of VP6 and 531-554 of VP4 are implicated in heat shock cognate protein hsc70-mediated rotavirus infection. PMID: 17876681
  97. an association between Hsc70 and ASIC2 that may underlie the increased retention of ASIC2 in the endoplasmic reticulum of glioma cells. PMID: 17878160
  98. HSP70 was a useful indicator of stressed neurons in acute phase of epilepsy, but not associated with neuronal death PMID: 17929041
  99. Our data show that a mutation in hsp70-Hom gene is associated with higher incidence of Postoperative atrial fibrillation. PMID: 17934269
  100. BAG-1 associates with Tau protein in an Hsc70-dependent manner. PMID: 17954934

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Subcellular Location Cytoplasm, Melanosome, Nucleus, nucleolus, Cell membrane
Protein Families Heat shock protein 70 family
Tissue Specificity Ubiquitous.
Database Links

HGNC: 5241

OMIM: 600816

KEGG: hsa:3312

STRING: 9606.ENSP00000227378

UniGene: Hs.180414

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