Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

MRC1

Uniprot No.

P22897

Research Area

Immunology

Alternative Names

bA541I19.1; C type lectin domain family 13 member D; C-type lectin domain family 13 member D; CD 206; CD206; CD206 antigen; CLEC13D; CLEC13DL; Macrophage mannose receptor 1; Macrophage mannose receptor 1 like protein 1; Macrophage mannose receptor; Mannose receptor C type 1; Mannose receptor C type 1 like 1; MMR; MRC 1; MRC1; MRC1_HUMAN; MRC1L1; OTTHUMP00000045206

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

655-1213aa

Target Protein Sequence

RTSLCFKLYAKGKHEKKTWFESRDFCRALGGDLASINNKEEQQTIWRLITASGSYHKLFWLGLTYGSPSEGFTWSDGSPVSYENWAYGEPNNYQNVEYCGELKGDPTMSWNDINCEHLNNWICQIQKGQTPKPEPTPAPQDNPPVTEDGWVIYKDYQYYFSKEKETMDNARAFCKRNFGDLVSIQSESEKKFLWKYVNRNDAQSAYFIGLLISLDKKFAWMDGSKVDYVSWATGEPNFANEDENCVTMYSNSGFWNDINCGYPNAFICQRHNSSINATTVMPTMPSVPSGCKEGWNFYSNKCFKIFGFMEEERKNWQEARKACIGFGGNLVSIQNEKEQAFLTYHMKDSTFSAWTGLNDVNSEHTFLWTDGRGVHYTNWGKGYPGGRRSSLSYEDADCVVIIGGASNEAGKWMDDTCDSKRGYICQTRSDPSLTNPPATIQTDGFVKYGKSSYSLMRQKFQWHEAETYCKLHNSLIASILDPYSNAFAWLQMETSNERVWIALNSNLTDNQYTWTDKWRVRYTNWAADEPKLKSACVYLDLDGYWKTAHCNESFYFLCK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

69.9 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human MRC1 is expressed in E.coli. It carries an N-terminal 6xHis-tag. Its expression region corresponds to the 655-1213aa of the human MRC1. Its purity exceeds 85% as measured by SDS-PAGE. The SDS-PAGE experiments showed MRC1's apparent molecular weight to be 66-80 kDa. It is suitable for the MRC1-related immunology research.

Human MRC1, also called CD206, is a type I integral membrane glycoprotein predominantly expressed on macrophages and dendritic cells. It plays a crucial role in the immune response by recognizing and binding to various carbohydrate structures, particularly those containing mannose, fucose, or N-acetylglucosamine.

MRC1 is primarily involved in the endocytosis and phagocytosis of pathogens, such as fungi and bacteria, which display high densities of mannose on their surfaces [1][2]. It also plays a role in clearing glycoproteins and other macromolecules from the extracellular environment, contributing to homeostasis and immune regulation [3][4].

References:
[1] G. Gaziri, L. Gaziri, R. Kikuchi, J. Scanavacca, & I. Felipe, Phagocytosis of candida albicans by concanavalin-a activated peritoneal macrophages, Medical Mycology, vol. 37, no. 3, p. 195-200, 2008. https://doi.org/10.1111/j.1365-280x.1999.00220.x
[2] I. Porcaro, M. Vidal, S. Jouvert, P. Stahl, & J. Gadrey, Mannose receptor contribution to candida albicans phagocytosis by murine e-clone j774 macrophages, Journal of Leukocyte Biology, vol. 74, no. 2, p. 206-215, 2003. https://doi.org/10.1189/jlb.1202608
[3] S. Swain, S. Lee, M. Nussenzweig, & A. Harmsen, Absence of the macrophage mannose receptor in mice does not increase susceptibility topneumocystis cariniiinfection in vivo, Infection and Immunity, vol. 71, no. 11, p. 6213-6221, 2003. https://doi.org/10.1128/iai.71.11.6213-6221.2003
[4] E. Song, M. Manganiello, Y. Chow, B. Ghosn, A. Convertine, P. Staytonet al., In vivo targeting of alveolar macrophages via raft-based glycopolymers, Biomaterials, vol. 33, no. 28, p. 6889-6897, 2012. https://doi.org/10.1016/j.biomaterials.2012.06.025

Target Background

Function

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.; (Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.; (Microbial infection) Acts as a receptor for Dengue virus envelope protein E.; (Microbial infection) Interacts with Hepatitis B virus envelope protein.

Gene References into Functions

Structural Insights into the pH-dependent conformational change and collagen recognition of the human mannose receptor have been reported. PMID: 29225077
no significant difference between our population of Egyptian SCD cases and controls regarding CD209 A>G polymorphism. Infections occurred more frequently among the heterozygous genotype (AG) and homozygous genotype (GG) patients. PMID: 29100978
A combination of CD68/CD206 density and HBV-positivity improves further predictive value for post-operative recurrence of hepatocellular carcinoma (HCC) . Quantification of CD68/CD206 macrophages and their distribution can be exploited for better postsurgical management of HCC patients. PMID: 28656201
The results showed that the small dense LDL particles reduce the MRC1 gene expression levels in the differentiated M2 macrophages from patients with coronary artery disease. Furthermore, they had high inflammatory capacity for the secretion of histamine. PMID: 28059035
Soluble CD206 plasma levels in rheumatoid arthritis reflect decrease in disease activity. PMID: 28598681
Loss of antral CD206-positive anti-inflammatory macrophages is a key feature in human gastroparesis and it is associates with interstitial cells of Cajal loss. PMID: 28066953
The effect of LPS in the expression of CD163 and CD206 on monocytes is not reverted in LPS tolerant cells, and the inhibition of inflammatory cytokines in tolerant cells is not related with modulation of these receptors PMID: 26352275
regulatory effect of the mannose receptor (MR) was mediated by a direct interaction with CD45 on the T cell, inhibiting its phosphatase activity, which resulted in up-regulation of CTLA-4 and the induction of T-cell tolerance. Inhibition of CD45 prevented expression of B-cell lymphoma 6 (Bcl-6), a transcriptional inhibitor that directly bound the CTLA-4 promoter and regulated its activity PMID: 27601670
1,25(OH)2D3 upregulates mannose receptor expression and enhances the monocyte/macrophage phagocytic potential in pulmonary tuberculosis. PMID: 27449998
Peritoneal dendritic cellss in endometriosis tissue express high levels of MR, which promotes phagocytosis of dead endometrial cells and thereby contributes to the etiology of endometriosis. PMID: 27793384
Mannose Receptor and CLEC5A on Macrophage first attracts the Dengue Virus with high avidity, and the virus concurrently interacts with CLEC5A in close proximity to form a multivalent hetero-complex and facilitate CLEC5A-mediated signal transduction. Dengue Virus Infection Is through a Cooperative Interaction between a Mannose Receptor and CLEC5A on Macrophage as a Multivalent Hetero-Complex. PMID: 27832191
Our work demonstrates a key role for MR in the modulation of the TLR4-AhR-IDO axis, which has a significant effect on DC behavior and the development of immune responses against allergens. PMID: 26703454
MRC1 and S100A9 may contribute to the determination of the mechanisms and screening involved in CRC. PMID: 26682511
Findings show that CD206 was highly expressed in the hepatocellular carcinoma (HCC) tissues and indicate that CD206 is a potential biomarker for predicting HCC prognosis. PMID: 26352004
Data show that umbilical cord-derived mesenchymal stem cells (UCMSCs) transplantation promoted the expression of CD206 antigen and increased phagocytic activity of macrophages through interleukin-6 (IL-6) in systemic lupus erythematosus (SLE) patients. PMID: 26209923
Data indicate that mannose receptor (CD206) is a major binding receptor for gamma-tilmanocept. PMID: 26202986
CD206 is a novel biomarker of alternative macrophage activation and treatment outcome in multiple myeloma. PMID: 26169445
The genome wide significant association of MRC1 with sCOMP levels was found likely to act independent of osteoarthritis subtypes. PMID: 25057126
CD206+ cells differentiate between influenza A (H1N1)pdm09 and seasonal influenza virus in lung tissue of fatal cases PMID: 25614715
TAMs phenotypes of glioma samples are the potential biomarkers in assessing the degree of malignancy, tumor invasion, and patient prognosis in clinic. PMID: 25182001
Increased expression of CD206 was found in perivascular macrophages from HIV-infected brain. PMID: 25146376
MRC-1 SNP, rs691005 was found to be associated with sustained viral response in HCV-1-infected patients; identified an interaction between MRC-1 rs691005 and IL-28B rs8099917 and response PMID: 24969847
presence of soluble form of MR in human serum in critical illness PMID: 24114918
There was a significant negative correlation between the number of CD163(+), CD204(+) or CD206(+) alveolar macrophages. PMID: 24498098
The MRC1/CD68 ratio is positively associated with adipose tissue lipogenesis and with muscle mitochondrial gene expression in humans. PMID: 23951013
Novel MRC1 gene polymorphisms are associated with susceptibility to pulmonary tuberculosis in Chinese Uygur and Kazak populations. PMID: 23653008
confers responsivenes to CpG-motif containing oligodeoxynucleotides in macrophages PMID: 24184555
Data suggest a role for CD206 in regulating allergen induced allergic responses in asthma. PMID: 23734186
Report CD44/macrophage mannose receptor as a ligand/receptor pair involved in the process of lymphocyte transit via the lymphatic vasculature. PMID: 23603511
The presence of human mannose receptor in a smaller number of vaginal epithelial cells of serodiscordant females prevented binding and HIV entry into these cells and therefore prevented sexual transmission of HIV. PMID: 23148569
The molecular association of the macrophage mannose receptor (MR) with HSP70 family members via the receptor cytoplasmic tail may contribute to MR trafficking in macrophages. PMID: 23345393
The mannose receptor found on the novel hybridoma cell has endocytic characteristics consistent with and similar to the mannose receptor found on the surface of monocyte-derived human macrophages. PMID: 22967244
results failed to confirm the reported association between MRC1 variant rs1926736 and IFNG variant rs2430561 and leprosy in Han Chinese; however variants rs692527 and rs34856358 of the MRC1 gene were associated with paucibacillary leprosy and rs3138557 of the IFNG gene was significantly associated with multibacillary leprosy PMID: 22392581
elderly subjects had twofold higher CD68 and CD206 gene expression (both P < 0.002) than young participants. In both studies, CD68(+) muscle macrophages were not associated with BMI PMID: 22314623
The frequency of MRC1 allele G1186A (rs34039386)in a Chinese population was higher in the pulmonary tuberculosis group than controls. No association was found for G1195A, T1212C, C1221G, C1303T or C1323T. PMID: 22393309
Sugar moieties on ADAMTS13 interact with MR, thereby promoting its endocytosis by antigen presenting cells. PMID: 22289891
MMR distribution in liver, spleen, lung, kidney, heart, diaphragm, quadriceps, and triceps in these animal models and compared them with MMR distribution in wild-type mice. PMID: 21416197
Studies show that tumoral mucin-mediated ligation of the MR on infiltrating TAM may contribute to their immune suppressive phenotype. PMID: 21331365
The studies provide the first demonstration of a significant role for mannose receptor, synergistic with TLR2, in activating a proinflammatory response to P. aeruginosa infection. PMID: 19197942
These data suggest that in hepatitis B virus infected patients, mannose receptor-mediated interaction between HBsAg and dendritic cells and subsequent impairment of dendritic cells predominantly occurs at the main site of infection, the liver. PMID: 19683778
This is the first study to imply that genetic variants in MRC1, a major member of the C-type lectin, contribute to the development of sarcoidosis. PMID: 21029423
MR plays a key role in the T helper type (Th)2 cell polarization observed after dust mite glycoallergen Der p 1 exposure through regulation of indoleamine 2,3-dioxygenase activity. PMID: 20610655
Data identify a new molecular pathway that links engagement of the mannose receptor, an important pattern recognition receptor for M. tuberculosis, with PPARgamma activation, which regulates the macrophage inflammatory response. PMID: 20554962
The mannose receptor and hCG were colocalized on the surface of uterine natural killer cells. PMID: 19196802
A significant association of exon 7 encoded amino acid haplotypes with leprosy per se (P = 0.012) and multibacillary leprosy (P = 0.004). PMID: 20035344
Results suggest that sequence variations in the MRC1 gene are associated with the development of asthma in two independent and ethnically diverse populations. PMID: 19902202
reduced expression in Mycobacterium tuberculosis-infected human macrophages exhibiting enhanced cellular adhesion PMID: 12368450
Engagement of the endocytic C-type 1 mannose receptor elicits a secretory program in dendritic cells characterized by a repertoire of anti-inflammatory cytokines and chemokines and the ability to inhibit the generation of Th1-polarized immune responses. PMID: 14568928
alveolar macrophage mannose receptor mediates Pneumocystis mediated NF-kB nuclear translocation and may represent an important mechanism of the host cell response to Pneumocystis infection PMID: 15155616
Endocytosis of glycosylated protein by the mannose receptor does not enhance presentation of antigen. The postulated role of the mannose receptor in presentation of glycoprotein-derived antigen is reevaluated in light of these results. PMID: 15190005

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Subcellular Location

Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein.

Database Links

HGNC: 7228

OMIM: 153618

KEGG: hsa:4360

STRING: 9606.ENSP00000239761

UniGene: Hs.743903

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Code	CSB-EP014783HUa0
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP014783HUa0 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MRC1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP014783HUa0 could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MRC1.
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Recombinant Human Macrophage mannose receptor 1 (MRC1), partial

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