Recombinant Human Mastermind-like protein 1(MAML1) ,partial

Code CSB-YP838784HU
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Source Yeast
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Code CSB-EP838784HU
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Source E.coli
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Code CSB-EP838784HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838784HU
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Source Baculovirus
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Code CSB-MP838784HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names MAML1
Uniprot No. Q92585
Alternative Names Mam-1; Mam1; MAML 1; Maml1; MAML1_HUMAN; Mastermind like 1; Mastermind-like protein 1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Gene References into Functions
  1. Our results showed that MEIS1 may have a negative role in regulation of MAML1expression during the esophageal squamous cell carcinoma progression. PMID: 28462489
  2. Authors report that p300 and CBP acetylate Mastermind-like 1 (Maml1) on amino acid residues K188 and K189 to recruit NACK to the Notch1 ternary complex, which results in the recruitment of RNA polymerase II to initiate transcription. PMID: 28625977
  3. Overexpression of Mastermind like1 was detected in 59% of tumor samples PMID: 28325367
  4. MAML1 may play an important role in tumor progression of Hepatocellular Carcinoma. PMID: 27650617
  5. The transcriptional coregulator MAML1 affects DNA methylation and gene expression patterns in human embryonic kidney cells. PMID: 26857655
  6. MMAL1 overexpression is associated with Esophageal Squamous Cell Carcinoma. PMID: 26294058
  7. study identifies that MAML1 is ubiquitinated in the absence of Notch signaling to maintain low levels of MAML1 in the cell PMID: 26225565
  8. In MCF-7 cells p53 associates with the Notch transcriptional complex (NTC) in a MAML1-dependent fashion, most likely through a p53-MAML1 interaction. PMID: 26033683
  9. The impact of MAML1 genetic variants to heart rate was discovered. PMID: 24680774
  10. Data indicate that EpCAM, CK19, and hMAM triple-marker-positive circulating tumor cells (CTCs) were detected in 86 of 98 (87.8 %) patients. PMID: 22990361
  11. Snail decreased transcription of Notch1 intracellular domain (NICD) target genes via competing with MAML1, co-activator, in NICD complex. PMID: 23454378
  12. Authors report that human papillomavirus type 8 E6 subverts NOTCH activation during keratinocyte differentiation by inhibiting RBPJ/MAML1 transcriptional activator complexes at NOTCH target DNA. PMID: 23365452
  13. This study demonstrated that targeting Maml1-induced tumor cell senescence and differentiation may alter the tumor microenvironment and cytokine and chemokine profiles and may also promote innate and adaptive immune cell infiltration and function. PMID: 22864395
  14. Bioinformatics assessment revealed a correlation between p300, EGR1 and MAML1 copy number and mRNA alterations in renal clear cell carcinoma and p300, EGR1 and MAML1 gene alterations were associated with increased overall survival. PMID: 23029358
  15. MAML1 is best known as the co-activator and effector of NOTCH-induced transcription, and BPV-1 E6 represses synthetic NOTCH-responsive promoters, endogenous NOTCH-responsive promoters, and is found in a complex with MAML1 in stably transformed cells PMID: 22249263
  16. Overexpression of MAML-1 and Twist1 were significantly associated with lymph node metastasis and the surgical staging of tumor PMID: 22006371
  17. Association of CSL with NICD exerts remarkably little effect on the exchange kinetics of the ANK domain, whereas MAML1 binding greatly retards the exchange kinetics of ANK repeats 2-3. PMID: 22325781
  18. MAML1 increases Notch acetylation by potentiating p300 autoacetylation. PMID: 22100894
  19. To target Notch signaling using MAML1 treatment may present a novel method to control cell viability in cervical cancer cells. PMID: 21640102
  20. Data show no detectable difference in the DNA binding site preferences of CSL before and after loading of four different Notch receptors and MAML1 proteins. PMID: 21124806
  21. SUMOylation of MAML1 is a mechanism for repressing MAML1 activity by influencing its interaction with HDAC7 PMID: 20203086
  22. Studies indicate that MAML1 functions as a coactivator for the tumor suppressor p53, MEF2C, beta-catenin and Notch signaling. PMID: 19751190
  23. Data support a model in which Notch-1 can activate the transcription of ERalpha-target genes via IKKalpha-dependent cooperative chromatin recruitment of Notch-CSL-MAML1 and ERalpha, which promotes the recruitment of p300. PMID: 19838210
  24. Mastermind mediates chromatin-specific transcription and turnover of the Notch enhancer complex PMID: 12050117
  25. requirement for cooperative assembly of the MAML1.ICN.CSL.DNA complex suggests that a primary function of ICN is to render CSL competent for MAML loading. PMID: 12644465
  26. MAML1 recruits CycC:CDK8 to phosphorylate the Notch ICD and coordinate activation with turnover. PMID: 15546612
  27. Results report the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1, the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). PMID: 16530044
  28. MAML1 has a coactivator function for p53, independent of its function as a coactivator of Notch signaling pathway PMID: 17317671
  29. Maml1 participates in the Wnt signaling by modulating the beta-catenin/TCF activity. Maml1 is recruited by beta-catenin on the cyclin D1 and c-Myc promoters. Maml1 functions in the Wnt/beta-catenin pathway independently of Notch signaling. PMID: 17875709
  30. the RBP-Jkappa-associated domain of Notch increases the effective concentration of the ankyrin domain for its binding site on CSL, enabling docking of the ankyrin domain and subsequent recruitment of the Mastermind-like coactivator. PMID: 18155729

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Subcellular Location Nucleus speckle
Protein Families Mastermind family
Tissue Specificity Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Database Links

HGNC: 13632

OMIM: 605424

KEGG: hsa:9794

STRING: 9606.ENSP00000292599

UniGene: Hs.631951

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