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| Code | CSB-EP850851HUa0 |
| Size | $2466 |
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| Description |
The full-length cDNA ORF of Human Mixed lineage kinase domain-like protein (MLKL) was N-terminally tagged a 6xHis and then expressed in E.coli. The Recombinant Human MLKL obtained got over 85% of purity determined by SDS-PAGE. Under reducing conditions, the MLKL protein migrated to an approximately 59 kDa molecular mass band on the gel. And stored MLKL proteins are offered. This recombinant MLKL protein may find uses in the specific antibody synthesis or the studies of MLKL-related signal transduction. MLKL is the terminal executor of necroptosis. In addition to the necroptotic function, MLKL is also involved in diverse biological processes. It controls the degradation of the myelin sheath, subsequently facilitating nerve regeneration. Studies have shown that MLKL controls NLRP3 inflammasome formation and IL-1β release in macrophages upon TLR activation. Moreover, MLKL is also involved in the maintenance of the integrity of cellular membrane ＆ the promotion of cell survival, the regulation of the expression of endothelial adhesion molecules, the control endothelial cell (EC)-leukocyte interaction in vitro and in vivo. |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Target Names | MLKL |
| Uniprot No. | Q8NB16 |
| Research Area | Signal Transduction |
| Alternative Names |
9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN
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| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 1-471aa |
| Target Protein Sequence | MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 58.5 kDa |
| Protein Length | Full Length |
| Tag Info |
N-terminal 6xHis-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.
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| Gene References into Functions |
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| Subcellular Location | Cytoplasm. Cell membrane. Nucleus. |
| Protein Families | Protein kinase superfamily |
| Database Links |
HGNC: 26617 OMIM: 615153 KEGG: hsa:197259 STRING: 9606.ENSP00000308351 UniGene: Hs.119878 |
