Code | CSB-RA850851A358phHU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
CUSABIO cloned MLKL antibody-coding genes into plasma vectors and then transfected these vector clones into mammalian cells using a lipid-based transfection reagent. Following transient expression, the recombinant antibodies against MLKL were harvested and characterized. The recombinant MLKL antibody was purified by affinity-chromatography from the culture medium. It can be used to detect MLKL protein from Human in the ELISA, IHC.
MLKL belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain. It is considered inactive because it lacks several residues required for activity. Diseases associated with MLKL include young adulthood diabetes and inflammatory bowel disease. Its related pathways include DNA damage response and regulation of c-FLIP. According to some studies, MLKL may have the following characteristics.
MLKL is a functional RIP3 substrate that binds to RIP3 through its kinase-like structure, but lacks kinase activity itself. RIP3 phosphorylates MLKL at T357 and S358. Modification of MLKL is critical for dissemination of the necrotic pathway downstream of RIPK3. Plasma membrane transport of trimeric MLKL protein is required for TNF-induced necrosis. In the absence of MLKL, RIPK3 promotes cell death and activation of the NLRP3 inflammasome. MLKL plays an important role in the necrosis of macrophages and MEFs.
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