Recombinant Human Neurofilament light polypeptide(NEFL)

In Stock
Code CSB-EP015688HU
Product Type Recombinant Protein
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P07196
Relevance Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015688HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFL.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP015688HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NEFL.
Storage Buffer Tris-based buffer,50% glycerol
Alias 68 kDa neurofilament protein Neurofilament triplet L protein
Species Homo sapiens (Human)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
Research Area others
Source E.coli
Gene Names NEFL
Protein Names Recommended name: Neurofilament light polypeptide Short name= NF-L Alternative name(s): 68 kDa neurofilament protein Neurofilament triplet L protein
Expression Region 2-543aa
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Mol. Weight 81.4kDa
Protein Description Full Length of Mature Protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Neurofilaments usually contain three intermediate filament proteins
Involvement in disease Charcot-Marie-Tooth disease 1F (CMT1F); Charcot-Marie-Tooth disease 2E (CMT2E)
Protein Families Intermediate filament family
Database Links

HGNC: 7739

OMIM: 162280

KEGG: hsa:4747

UniGene: Hs.521461

Q&A and Customer Reviews

Q&A
Q:

Are both tags, i.e. 10xHis-SUMO tag at the N-terminus and Myc tag at the C-terminus used for purification? Is the SUMO tag removed by SUMO Protease treatment?

A:
Thanks for your inquiry. Here is the informaton about tags:
① The His changes from 6*his to 10*his. The main reason is that 10*His has a stronger affinity for the column material whih is helpful for those proteins that can't attach to the column very well.
It is also helpful for protein purification (The specific adsorption capacity target protein is strong, and the nonspecific adsorption ability of the impurity protein is weak, then we can design a high concentration of midazolam to further remove the impurity protein).
② We add myc-tag at C-terminus. This is mainly for the convenience of customers and we to do WB detection for myc-tag antibody, thus further test the accuracy of the target protein. Although we can also detect His-tag antibodies by WB, but relatively speaking, its specificity is not good as myc-tag.
③ We add thrombin site for all tags. It will be more convenient if you require tag removal later.
Q:

I was wondering if you could let me know at what concentration the current lot of CSB-EP015688HU / Recombinant Human Neurofilament light polypeptide(100 µg) is provided.

A:
Thanks for your inquiry. Regarding this item, CSB-EP015688HU, we have the protein both in liquid form and lyophilized form currently.
Liquid form: 0.32mg in stock. Lot number is 03193. The concentration is 0.8mg/ml.
Lyophized form: 2.44mg in stock. Lot number is 03403. The concentration before lyophization is 0.6mg/ml. You could redissolve it to your wanted concentration.
Customer Reviews

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