Human Neurofilament protein L (NF-L) ELISA kit

Code CSB-E16094h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07196
Lead Time 3-5 working days
Abbreviation NEFL
Target Name neurofilament, light polypeptide
Alias CMT1F, CMT2E, FLJ53642, NF-L, NF68, NFL, light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, lig
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
HGNC 7739
RGD 621458
MGI 97313
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NF-L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 99  
Range % 91-105  
1:10 Average % 85  
Range % 80-92  
1:20 Average % 88  
Range % 82-94  
1:40 Average % 93  
Range % 86-98  
Recovery
The recovery of human NF-L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 98 92-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.770 2.665 2.718 2.628  
1000 2.114 2.006 2.060 1.970  
500 1.256 1.243 1.250 1.160  
250 0.657 0.651 0.654 0.564  
125 0.343 0.339 0.341 0.251  
62.5 0.252 0.242 0.247 0.157  
31.25 0.209 0.198 0.204 0.114  
0 0.092 0.088 0.090    
References
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Function Neurofilaments usually contain three intermediate filament proteins
Involvement in disease Charcot-Marie-Tooth disease 1F (CMT1F); Charcot-Marie-Tooth disease 2E (CMT2E)
Protein Families Intermediate filament family
Database Links

HGNC: 7739

OMIM: 162280

KEGG: hsa:4747

UniGene: Hs.521461

Q&A and Customer Reviews

Q&A
Q:

The Cusabio ELISA kit CSB-E16094h, does not name the source of the standard. Can you tell us which standard is used here?
Could you send us linearity and parallelism data for this ELISA?

A:
Thanks for your inquiry.
The standard of this kit is 293 cell-derived recombinant protein.
As for the linearity and parallelism data,I will send it to you via e-mail.Pls let me know if you have any further questions. Thank you.
Customer Reviews

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