Recombinant Mouse Bcl-2 homologous antagonist/killer (Bak1), partial

Code CSB-YP002548MO1
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Source Yeast
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Code CSB-EP002548MO1
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Source E.coli
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Code CSB-EP002548MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002548MO1
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Source Baculovirus
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Code CSB-MP002548MO1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bak1
Uniprot No.
Alternative Names
Bak1; Bak; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2.
Gene References into Functions
  1. It was shown that double knockout of Bax and Bak from proximal tubules attenuated renal tubular cell apoptosis and suppressed renal interstitial fibrosis in UUO. PMID: 28317867
  2. Study reports the proximal alpha1-alpha2 loop as a second activation site in Bak and in mitochondrial Bax. PMID: 27217060
  3. Here the authors show that mouse embryonic fibroblasts deficient in Bax/Bak1 are resistant to the third major form of cell death associated with autophagy through a mechanism involving lysosome permeability. PMID: 29148970
  4. BH3-only proteins bind inactive full-length BAK at mitochondria and then dissociate following exposure of the BAK BH3 and BH4 domains before BAK homodimerization PMID: 28673969
  5. Resultdetermined the mouse Bak BH3-in-groove homodimers (BGH) structure, which allowed more accurate modeling within and between the BGHs. The BGHs were shown to oligomerize via the 'alpha 3/alpha 5 interface' in mitochondria. These findings suggest for a probable assembly of the Bak homodimers in the mitochondrial apoptotic pore. PMID: 27488021
  6. We propose that the GC-induced mitochondrial accumulation of Bax and the interaction between the GR and Bim, Bcl-xL and Bak could play a role in the regulation of thymocyte apoptosis. PMID: 27888447
  7. Conversion of Bim-BH3 from Activator to Inhibitor of Bak through Structure-Based Design. PMID: 29149594
  8. Postnatal synaptic rearrangement needed for acquisition of skilled behaviors requires the activity-dependent, non-apoptotic Bax/Bak-caspase signaling cascade. Adult Bax/Bak mutant mice exhibit aberrant co-activation of antagonistic muscle pairs and skilled grasping deficits but normal reaching and retrieval behaviors. PMID: 28472660
  9. The authors show that Bak is activated and that activated Bak is bound to p53 during reovirus encephalitis. PMID: 27307572
  10. Pancreatic beta-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak. PMID: 27137932
  11. lipid profile in the absence of the proapoptotic proteins BAX and BAK in mouse embryonic fibroblasts PMID: 26059977
  12. alpha1 dissociation is a key step in unfolding Bak into three major components, the N terminus, the core (alpha2-alpha5) and the latch (alpha6-alpha8) that is required for apoptosis. PMID: 25880232
  13. BAX and BAK have a nonapoptotic role in eicosanoid metabolism in inflammation. PMID: 25815636
  14. Puma is the major mediator of virus-induced Bax/Bak activation and mitochondrial membrane permeabilization induction. PMID: 26030884
  15. Motifs of VDAC2 required for mitochondrial Bak import and tBid-induced apoptosis. PMID: 26417093
  16. dual ablation of Bax and Bak suppressed ureteral obstruction induced inflammation and kidney fibrosis with decreased tubular cell cycle arrest PMID: 26180237
  17. bak therefore regulates gastric epithelial cell apoptosis, proliferation, differentiation, mucosal thickness, and susceptibility to gastric atrophy and dysplasia following H. felis infection PMID: 26159699
  18. Benzo(a)pyrene-7,8-diol-9,10-epoxide induced p53-independent necrosis via the mitochondria-associated pathway involving Bax and Bak activation PMID: 24837741
  19. MCL1, but not that of BAK, forms stable heterodimeric complexes with cBID in a manner adjustable by membrane cardiolipin content and curvature degree. PMID: 25987560
  20. data predict that the MPTP is an inner membrane regulated process, although in the absence of Bax/Bak the outer membrane resists swelling and prevents organelle rupture to prevent cell death PMID: 23991283
  21. Abeta oligomers bind to BAK on the membrane and induce apoptotic BAK pores and cytochrome c release PMID: 25296312
  22. results suggest that both of PDI and PDIA3 possess Bak-dependent proapoptotic function through inducing mitochondrial outer membrane permeabilization, which provides a new mechanism linking ER chaperone proteins and apoptotic signaling PMID: 25697356
  23. Platelet life span was found to be elevated in vavP-BCL-2 mice, which should have provoked thrombocytosis, as in Bak(-/-) mice. PMID: 24464220
  24. The ATF3 lies downstream of JNK signaling after TLR engagement, resulting in repression of pro-apoptotic Bak and Bax transcription. PMID: 23697557
  25. Data indicate that the antiapoptotic Bcl-2 family members do not directly inhibit components of the autophagic pathway but instead affect autophagy indirectly, owing to their inhibition of Bax and Bak. PMID: 24912196
  26. Gene-knockout studies support a critical role of Bax and Bak in tubular cell apoptosis in ischemic acute kidney injury. PMID: 23466994
  27. The results provide further insights into the organization of the BAK oligomeric pores by the BAK homodimers during mitochondrial apoptosis, enabling the proposal of a BAK-induced lipidic pore with the topography of a "worm hole." PMID: 24337568
  28. these results provide strong evidence that Puma, like Bim, Noxa, and tBid, is able to act as a direct Bak activator. PMID: 24265320
  29. N-Bak mRNA is translationally repressed by multiple mechanisms. PMID: 23969856
  30. Data indicate that Mcl-1 deficient embryonic fibroblasts (MEFs) reliant only on Bcl-XL for survival, and Bax/Bak deficient MEFs support a mechanism-based induction of apoptosis. PMID: 23767404
  31. Combined loss of BOK and BAK does not elicit any noticeable defects, although it remains possible that BOK and BAK have critical roles in developmental cell death. PMID: 23744350
  32. Bak apoptotic pore forms by the multimerization of BH3:groove homodimers and reveals that Bak alpha6 is not only important for Bak oligomerization and function but may also be involved in how Bak is activated by BH3-only proteins. PMID: 23893415
  33. role of BAX/BAK in long-term regulation of Nestin-positive progenitor cell pools, with loss of function predisposing to adult-onset tumorigenesis. PMID: 22986529
  34. Altered mitochondrial morphology and defective protein import in Bax/Bak-deficient mice reveal novel roles for Bax and/or Bak in the skeletal muscle. PMID: 23784543
  35. Murine cytomegalovirus-mediated inhibition of the pro-apoptotic Bak protein is required for optimal in vivo replication. PMID: 23468630
  36. Demonstrate that P2Y(12) activation protects platelets from apoptosis via PI3k-dependent Bak/Bax inactivation. PMID: 23140172
  37. activation of both BAK and BAX is initiated by direct BH3-interaction but at distinct trigger sites. PMID: 23404709
  38. Bak plays a critical role while Bax has an ancillary role in safeguarding immunological tolerance and prevention of autoimmune disease. PMID: 23349374
  39. The p53-Bak apoptotic signaling axis plays an essential role in regulating lens differentiation. PMID: 22671997
  40. BAX/BAK-independent cell death did not require Cyclophilin D (CypD) expression, an important regulator of the mitochondrial permeability transition pore PMID: 22719850
  41. Deletion of Bak significantly inhibited hepatocyte apoptosis in Mcl-1 KO mice and reduced the incidence of liver cancer. PMID: 22414765
  42. This study showed that denervation-induced muscle disuse activates both apoptotic and autophagic signaling pathways in muscle, and autophagic protein expression does not exhibit a compensatory increase in the presence of attenuated apoptosis. PMID: 22673615
  43. Withanolide D elicits apoptosis in malignant cells through Bax/Bak dependent pathway in p53-wild type cells, whereas Bak compensates against loss of Bax in p53-null cells PMID: 22479585
  44. Results indicate that a protective role for Bak during ionophore-induced cell death may be closely associated with its regulatory effect on maintenance of autophagic flux and vacuole homeostasis. PMID: 22493436
  45. Bax and Bak are functionally redundant, but they are counteracted by distinct anti-apoptotic Bcl-2 family proteins in different species PMID: 22056880
  46. the differential regulation of Bak and Bax by Mcl-1 is predominantly independent of the initial subcellular localizations of Bak and Bax. PMID: 22442658
  47. There is strong translational arrest of N-Bak mRNA in the neurons. This arrest is partially mediated by 50-untranslated region of Bak mRNA and it is not released during mitochondrial apoptosis. PMID: 22297299
  48. Data suggest that endoplasmic reticulum-localized Bcl2 protects against a Bax/Bak-independent cell death pathway initiated by the p20 fragment of Bap31. PMID: 22197342
  49. Studies suggest that BAK/BAX activation and apoptosis are coordinated through BH3-only proteins and a specific lipid milieu that is maintained by heterotypic membrane-mitochondrial interactions. PMID: 22385963
  50. Either Bak or Bax is critically required for rapid execution of Fas-mediated massive apoptosis in the liver, delayed onset of mitochondria-independent, caspase-dependent apoptosis develops even in the absence of both. PMID: 21425311

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Subcellular Location
Mitochondrion outer membrane; Single-pass membrane protein.
Protein Families
Bcl-2 family
Tissue Specificity
Widely expressed.
Database Links
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