Recombinant Mouse Eukaryotic translation initiation factor 4E-binding protein 1(Eif4ebp1)

Code CSB-YP733716MO
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Source Yeast
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Code CSB-EP733716MO
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Source E.coli
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Code CSB-EP733716MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733716MO
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Source Baculovirus
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Code CSB-MP733716MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Eif4ebp1
Uniprot No. Q60876
Alternative Names Eif4ebp1Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Species Mus musculus (Mouse)
Expression Region 2-117
Target Protein Sequence SAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR IIYDRKFLME CRNSPVAKTP PKDLPAIPGV TSPTSDEPPM QASQSQLPSS PEDKRAGGEE SQFEMDI
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Gene References into Functions
  1. 4E-BP proteins may prevent excess lipid accumulation in skeletal muscle and suggest that 4E-BPs are key regulators of muscle homeostasis regardless of insulin sensitivity. PMID: 28544394
  2. decreasing cap-dependent translation by expressing a constitutively active mutant of the translational repressor eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) prevents neuronal misplacement and soma enlargement, while partially rescuing dendritic hypertrophy induced by hyperactive mTORC1. PMID: 27647922
  3. we show that simultaneous inhibition of mTOR signaling to both S6K1 and 4E-BP1 is sufficient to reduce AKT-induced muscle growth and render it insensitive to the mTORC1-inhibitor rapamycin PMID: 27705797
  4. 4E-BP1 is a gender-specific suppressor of obesity that regulates insulin sensitivity and energy metabolism. PMID: 27498874
  5. The authors now show that West Nile virus growth and protein expression are dependent on mTORC1 mediated-regulation of the eukaryotic translation initiation factor 4E-binding protein/eukaryotic translation initiation factor 4E-binding protein (4EBP/eIF4E) interaction and eukaryotic initiation factor 4F (eIF4F) complex formation to support viral growth and viral protein expression. PMID: 27763553
  6. p-mTOR, p-4EBP1, HIF-1alpha and VEGF together are involved in the pathogenesis of asthma. PMID: 28100332
  7. Thus, translational control by 4E-BP1 downstream of mTOR effects the expression of neuroligin 1 and excitatory synaptic transmission in the spinal cord, and thereby contributes to enhanced mechanical nociception. PMID: 26678009
  8. The findings support a model whereby elevated 4E-BP1 expression observed in the retina of diabetic rodents is the result of O-GlcNAcylation of 4E-BP1 within its PEST motif. PMID: 26998719
  9. The mTORC1 effectors S6K1 and 4E-BP play different roles in CNS axon regeneration. PMID: 25382660
  10. Oxidative stress-induced premature senescence occurred due to impaired autophagy function through 4EBP1 phosphorylation. PMID: 25682865
  11. Data show that deletion of eukaryotic translation initiation factor 4E-binding protein 1/2 (4E-BP1/2) in erythroid cells rendered them resistant to mammalian target of rapamycin complex 1 (TORC1) inhibition and restored hemoglobin production. PMID: 25872869
  12. These findings indicate that regulation of 4E-BP1 in skeletal muscle may serve as an important conduit through which mTORC1 controls metabolism. PMID: 26121750
  13. Disruption of genes encoding Eif4ebp1 and Eif4ebp2 does not alter basal or sepsis-induced changes in skeletal muscle protein synthesis in male or female mice. PMID: 24945486
  14. mTORC1 also regulates HIF-1alpha synthesis on a translational level via co-operative regulation of both initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase-1 (S6K1) PMID: 24931163
  15. Translational repression of TOP mRNAs involves TSC and Rheb and hypophosphorylated state of 4E-BP. PMID: 24627160
  16. Overexpression of eIF4E, the proto-oncogene whose activity is specifically inhibited by 4EBP1, resulted in hepatocellular carcinoma development in cooperation with activated Ras. PMID: 25145583
  17. 4E-BP1 is a trigger for parthenolide -induced autophagy PMID: 25482447
  18. The 4E-BP1 may suppress brown adipocyte differentiation and PGC-1alpha expression in white adipose tissues. PMID: 23672244
  19. also seen in mitotic cells, indicating that EIF4EBP1 control of localized mRNA translation is likely key to correct segregation of genetic material across cell types. PMID: 23852387
  20. Loss of PINK1 impairs both hypoxia-induced 4E-BP1 dephosphorylation and increase in the ratio of internal ribosomal entry site dependent to cap-dependent translation PMID: 24553947
  21. The results of this study revealed a key role for mTOR/4E-BP1-mediated translational control in regulating entrainment and synchrony of the master clock. PMID: 23972597
  22. these data provide evidence for a novel mechanism whereby O-GlcNAcylation of 4E-BP1 mediates translational control of hepatic gene expression. PMID: 23434932
  23. PTEN/Akt signaling controls mitochondrial respiratory capacity through 4E-BP1 PMID: 23049865
  24. Elevated levels of 4E-BP1 may be involved in a mechanism to arrest brain development in Phgdh knock out embryos. PMID: 23350942
  25. A potential interaction of LRRK2 and 4E-BP1 in mammalian cells and brain, is investigated. PMID: 23082216
  26. in the absence of 4E-BP proteins, mTORC1-mediated phosphorylation of p70S6K1 is elevated by a reduction in competition between the two substrates for interaction with raptor PMID: 23184952
  27. Hyperglycemia-induced O-GlcNAcylation and truncation of 4E-BP1 protein in liver of a mouse model of type 1 diabetes. PMID: 21840999
  28. the inhibitory activity of 4E-BP1 is a key mediator of the negative effects of reduced mechanistic target of rapamycin signaling on cardiomyocyte homeostasis and heart function. PMID: 20644257
  29. 4E-BPs had no effect on cell size, but they inhibited cell proliferation by selectively inhibiting the translation of messenger RNAs that encode proliferation-promoting proteins and proteins involved in cell cycle progression PMID: 20508131
  30. Map4k4 negatively regulates PPARgamma post-transcriptionally, by attenuating mTOR signaling and a 4E-BP1-dependent mechanism PMID: 20038583
  31. These results, together with the fact that 4E-BPs are markedly induced during granulo-monocytic differentiation of myeloid cells in vitro, highlight the pivotal role of 4E-BP1 and 4E-BP2 in the early phases of myelopoiesis PMID: 19175792
  32. 4EBP1 is activated by the Type I IFN receptor-activated PI 3'-kinase pathway and has a role in regulating mRNA translation and induction of Type I IFN responses PMID: 12759354
  33. eIF4E is rate-limiting for cell cycle progression before it regulates cell growth PMID: 14607835
  34. eukaryotic initiation factor 4E-binding protein 1 gene transcription is regulated by polyamines PMID: 15351722
  35. Comparison of the p53-induced patterns of eIF4GI and 4E-BP1 dephosphorylation and cleavage with those caused by the mTOR inhibitor rapamycin indicates that p53 activation and rapamycin have distinct but additive effects PMID: 15897901
  36. formation of the Nrf-2/PMF-1 complex is involved in transcriptional regulation of 4E-BP1 PMID: 16647090
  37. These findings suggest that the ability of cells to rephosphorylate 4E-BP1, resynthesise eIF4GI and restore the rate of protein synthesis after inactivation of p53 is an important aspect of recovery following the relief of physiological stress. PMID: 16990847
  38. Increased insulin resistance in 4E-BP1 and 4E-BP2 double KO was associated with increased ribosomal protein S6 kinase (S6K) activity and impairment of Akt signaling in muscle, liver, and adipose tissue. PMID: 17273556
  39. All-trans-retinoic acid- or cis-RA-dependent p21(Waf1/Cip1) protein expression is enhanced in mouse embryonic fibroblasts with targeted disruption of the 4e-bp1 gene, in the absence of any effects on transcriptional regulation of the p21(Waf1/Cip1) gene. PMID: 18280804
  40. Pretreatment with cyanamide partially prevented the ethanol-induced lowering of 4EBP1 phosphorylation. PMID: 18317950
  41. endocrine hormone insulin contributes to VEGF release in skin wounds through an Akt1-and 4E-BP1- mediated posttranscriptional mechanism in keratinocytes PMID: 18668138
  42. Results show that overexpression of polycystin-1 in renal epithelial cells inhibits cell growth in a cell cycle-independent manner due to the downregulation of mTOR, S6K1, and 4EBP1, and through phosphorylation of tuberin. PMID: 19255143
  43. These results indicate that mTOR downstream effector 4EBP1 is essential for normal thymocyte proliferation, but deregulation of 4EBP1 in Atm deficiency is a major factor driving thymic lymphomagenesis in the animals. PMID: 19364503
  44. High-density lipoproteins can protect beta-cells through modulation of 4E-BP1 depending on the type of stress stimuli. PMID: 19574449
  45. Activated 4E-BP1 represses tumourigenesis and IGF-I-mediated activation of the eIF4F complex in mesothelioma. PMID: 19603014

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Protein Families EIF4E-binding protein family
Tissue Specificity Highest expression in fat cells.
Database Links

KEGG: mmu:13685

STRING: 10090.ENSMUSP00000033880

UniGene: Mm.6700

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