Recombinant Mouse Neurogenic locus notch homolog protein 2(Notch2) ,partial

Code CSB-YP015950MO
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Source Yeast
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Code CSB-EP015950MO
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Source E.coli
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Code CSB-EP015950MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015950MO
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Source Baculovirus
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Code CSB-MP015950MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Notch2
Uniprot No. O35516
Alternative Names Notch2; Neurogenic locus notch homolog protein 2; Notch 2; Motch B) [Cleaved into: Notch 2 extracellular truncation; Notch 2 intracellular domain]
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (By similarity).
Gene References into Functions
  1. that regulating Notch2 protein levels is particularly important during normal signaling during mouse lens development PMID: 30059908
  2. The results suggest that CD19 controls the differentiation of marginal zone precursors to marginal zone B cells by regulating ADAM28-mediated Notch2 cleavage. PMID: 28707394
  3. Notch2 physiologically regulates bone remodeling by inhibiting trabecular bone formation in the appendicular skeleton. PMID: 27102824
  4. Notch2 was crucial in maintaining the integrity of the epithelial and smooth muscle layers of the distal conducting airways, and our data suggest that epithelial Notch signaling regulates multiple aspects of postnatal development in the distal lung and may represent a potential target for intervention in pulmonary diseases. PMID: 27364009
  5. Authors confirmed that NOTCH2 is significantly over-expressed in EEA. In the most relevant endometrial adenocarcinoma cell model, Ishikawa H, altering miR-181c expression produces significant changes in NOTCH2 expression, consistent with direct targeting. PMID: 28969912
  6. These findings highlight the molecular basis of Hajdu-Cheney syndrome (HCS) pathogenesis and provide clinical insights into potential targeted therapeutic strategies for skeletal disorders associated with the aberrant FBW7/NOTCH2 pathway as observed in patients with HCS. PMID: 29149593
  7. Hajdu-Cheney Syndrome is a devastating disease associated with a gain-of-NOTCH2 function resulting in diverse clinical manifestations PMID: 27241678
  8. Study indicates that knockdown of Notch2 promotes in vivo growth of triple-negative breast cancer (TNBC) and proposes that Notch2 functions as a tumor growth suppressor in TNBC. PMID: 27097807
  9. Human biliary atresia and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-induced experimental cholestasis in mice are associated with increased expression of Notch2. PMID: 28688656
  10. The data showed that the overexpression of miR-18a-5p could downregulate Notch2 expression and subsequently suppress endothelial-mesenchymal transition and cardiac fibrosis. PMID: 28733035
  11. Transgenic mice harboring Notch2 mutation analogous to that in patients with Hajdu-Cheney syndrome (HCS) are severely osteopenic because of enhanced bone resorption; this model has now been validated. Data from further studies in transgenic mice suggest that the HCS mutation in osteoblasts, but not in osteoclasts, causes osteopenia in this model. PMID: 28592489
  12. this study shows that Notch2 activity is required for the maintenance of lung tissue-resident memory CD103+ T cells PMID: 27776108
  13. In this study, authors use a smooth muscle-specific deletion of Notch2 together with a global Notch3 deletion to produce mice with combinations of mutant and wild-type Notch2/3 alleles in vascular smooth muscle cells PMID: 26453897
  14. 6955C-->T mutation in the Notch2 locus leading to cancellous and cortical bone osteopenia. PMID: 26627824
  15. Data (including data from studies in transgenic/knockout mice) suggest deletion of Notch1 in podocytes prevents diabetic nephropathy development and up-regulates Notch2 expression; overexpression of Notch2 does not promote diabetic nephropathy. PMID: 26293507
  16. NOTCH2 inhibits PDGF-B-dependent proliferation and its expression is decreased by PDGF-B. PMID: 25957400
  17. BTK synergizes with Notch2 to govern marginal zone B cells in non-obese diabetic mice. PMID: 26034172
  18. Surface expression of NOTCH2 was seen in specified E11 AGM (CD45+VE-cadherin+) hematopoietic progenitors. NOTCH activation by Delta1 can increase long-term engrafting HSC numbers from these cells. PMID: 25866967
  19. drives multiple myeloma associated osteoclast development and bone destruction PMID: 25257302
  20. Hepatic Notch2 deficiency leads to bile duct agenesis perinatally and secondary bile duct formation after weaning. PMID: 25446530
  21. This study demonstrates important functions for Jagged1 and Notch2 in the resolution of germ cell syncytia and the coordination of somatic and germ cell growth within follicles of the mouse ovary. PMID: 24552588
  22. Loss of Notch2 has little influence on early embryonic pituitary proliferation but is crucial for postnatal progenitor maintenance. PMID: 24673559
  23. Propose a receptor-specific function for Notch2 in regulating Jag-1-induced p27(kip1) expression and growth arrest in vascular smooth muscle cells. PMID: 23965337
  24. Lack of Notch-2 prevents biliary tubule formation, both in vivo and in vitro. PMID: 23500150
  25. Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens. PMID: 23913046
  26. In the postnatal retina, NOTCH2, as well as the Notch downstream genes, HES1 and SOX9, were detected in VSX2/Cyclin D1/SOX2-expressing cells in the postnatal retina, and in the mature retina NOTCH2 was most abundant in Muller glia PMID: 23277114
  27. The N2 extracellular domain (NECD) increases Notch protein localization to the cell surface during kidney development and is cleaved more efficiently upon ligand binding. PMID: 23806616
  28. Notch2 appears to be the main determinant not only of biliary commitment of embryonic hepatoblasts during development but also of biliary reprogramming of adult hepatocytes. PMID: 23315998
  29. Notch2 regulates BMP signaling and epithelial morphogenesis in the ciliary body of the mouse eye. PMID: 23676271
  30. Notch2 signaling in NSCs produces features of GSCs and induces astrocytic lineage entry, consistent with a possible role in astrocytoma formation. PMID: 22717580
  31. Our data establish an oncogenic role for constitutive Notch2 signaling in liver cancer development PMID: 23175466
  32. Pubertal Notch2+ lineages play a critical role in the formation of alveolar clusters. PMID: 23604318
  33. demonstrates that Notch2-mediated signaling in the somatic-cell lineage of the mouse ovary regulates oocyte apoptosis non-cell autonomously, and is essential for regulating breakdown of germ-cell nests and formation of primordial follicles PMID: 23406467
  34. Osteoclastogenesis is enhanced following binding of monoclonal antibody to Notch2. PMID: 22390640
  35. expression of constitutively active Notch2 in the myocardium of transgenic mice resulted in hypertrabeculation, reduced compaction, and ventricular septum defects PMID: 22865640
  36. results suggest that two different assemblies of Notch receptors coordinate the numbers and distribution of the major epithelial cell types in the conducting airway during lung organogenesis PMID: 23132245
  37. In vitro analysis show that both Notch2 and Notch3 robustly activate smooth muscle differentiation genes, and Notch3, but not Notch2 is a target of Notch signaling PMID: 22615991
  38. These data identify Fos as one of the target genes for Notch2 signaling that is crucial for marginal zone B development. PMID: 22293205
  39. Notch2-mediated signaling regulates lens morphogenesis, apoptosis, cell cycle withdrawal, and secondary fiber cell differentiation. PMID: 22173065
  40. Data from transgenic/knockout mice indicate that Notch2 signaling acts as a binary switch that drives, depending on the cellular background, either T-cell development or the generation of marginal zone B-cells. PMID: 21795747
  41. Notch2 is a common differentiation signal for T cell-priming CD11b(+) DC subsets in the spleen and intestine. PMID: 22018469
  42. Notch signaling is involved in follicular development by regulating granulosa cell proliferation. PMID: 21427220
  43. Whereas fetal RORgammat(+) cells matured in the fetal liver environment, adult bone marrow-derived RORgammat(+) ILCs matured outside the bone marrow, in a Notch2-dependent manner PMID: 21909092
  44. Notch-2 was identified as a novel target for beta-catenin-dependent Wnt signaling. PMID: 21437251
  45. Notch2 has a role in assuring orderly repopulation by HSCs, MPPs, myeloid cells, and lymphoid cells during BM regeneration PMID: 21285514
  46. Loss of Notch2 is associated with impaired eradication of Strongyloides venezuelensis. PMID: 20971948
  47. Notch-2 up-regulation after neonatal ischemia is detrimental to neuronal survival. PMID: 20876509
  48. Notch2 activation did not skew the cell fate toward the proximal nephron but resulted in severe kidney dysgenesis and depletion of Six2-positive progenitors. PMID: 20299358
  49. ADAM10 regulates marginal zone b cell development by initationing notch2 signaling. PMID: 20156974
  50. Notch2 plays an important role in development of the roof plate of the diencephalon and mesencephalon, and cellular rearrangement is involved in this process PMID: 12242712

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Subcellular Location Notch 2 extracellular truncation: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Notch 2 intracellular domain: Nucleus, Cytoplasm
Protein Families NOTCH family
Tissue Specificity Expressed in the brain, liver, kidney, neuroepithelia, somites, optic vesicles and branchial arches, but not heart.
Database Links

KEGG: mmu:18129

STRING: 10090.ENSMUSP00000078741

UniGene: Mm.485843

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