Recombinant Rat Thyrotropin subunit beta(Tshb)

Code CSB-MP025130RA
Size Pls inquire other sizes
Source Mammalian cell
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Code CSB-EP025130RA-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Product Details

Purity >85% (SDS-PAGE)
Target Names Tshb
Uniprot No. P04652
Alternative Names TshbThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain
Species Rattus norvegicus (Rat)
Expression Region 21-132
Target Protein Sequence FCIPTEYMMY VDRRECAYCL TINTTICAGY CMTRDINGKL FLPKYALSQD VCTYRDFTYR TVEIPGCPHH VAPYFSYPVA LSCKCGKCNT DYSDCTHEAV KTNYCTKPQT FY
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Indispensable for the control of thyroid structure and metabolism.
Gene References into Functions
  1. These data add new insight to an important role of T3 in rapidly regulating TSH beta gene expression at posttranscriptional level. PMID: 21078364
  2. TSH action stimulates the phosphorylation of hsp-90 in FRTL-5 thyroid cells PMID: 16910874
  3. Results clearly show that there are diurnal changes in TSH expression and accumulation in rat PT-TSH cells and suggest that these fluctuations are regulated by melatonin. PMID: 17927662
Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: rno:25653

STRING: 10116.ENSRNOP00000022575

UniGene: Rn.9966

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