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Recombinant Rat Thyrotropin subunit beta (Tshb)

Recombinant Rat Thyrotropin subunit beta (Tshb)

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Code	CSB-EP025130RA
Abbreviation	Recombinant Rat Tshb protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Tshb

Uniprot No.

P04652

Research Area

Signal Transduction

Alternative Names

TshbThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

21-132aa

Target Protein Sequence

FCIPTEYMMYVDRRECAYCLTINTTICAGYCMTRDINGKLFLPKYALSQDVCTYRDFTYRTVEIPGCPHHVAPYFSYPVALSCKCGKCNTDYSDCTHEAVKTNYCTKPQTFY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

19.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To prepare this Recombinant Rat Tshb protein, the recombinant DNA was required, which was generated by fusing the Tshb gene with N-terminal 10xHis tag and C-terminal Myc tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this Tshb protein production. After purification, a premium Tshb recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+.

Tshb is a gene encoding a protein named thyrotropin subunit beta (also commonly calld TSH-B or TSH-beta). The protein encoded by this gene and the α subunit together makes up thyroid stimulating hormone (TSH), which stimulates the thyroid gland to produce thyroxine. Then, triiodothyronine stimulates the metabolism of almost every tissue in the body. In terms of TSH, α subunit is common to pituitary gonadotropins, whereas the β subunit is TSH-specific, providing receptor specificity.

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Target Background

Function

Indispensable for the control of thyroid structure and metabolism.

Gene References into Functions

These data add new insight to an important role of T3 in rapidly regulating TSH beta gene expression at posttranscriptional level. PMID: 21078364
TSH action stimulates the phosphorylation of hsp-90 in FRTL-5 thyroid cells PMID: 16910874
Results clearly show that there are diurnal changes in TSH expression and accumulation in rat PT-TSH cells and suggest that these fluctuations are regulated by melatonin. PMID: 17927662

Subcellular Location

Secreted.

Protein Families

Glycoprotein hormones subunit beta family

Database Links

KEGG: rno:25653

STRING: 10116.ENSRNOP00000022575

UniGene: Rn.9966

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