Greater than 85% as determined by SDS-PAGE.
TshbThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain
Rattus norvegicus (Rat)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Full Length of Mature Protein
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
To prepare this Recombinant Rat Tshb protein, the recombinant DNA was required, which was generated by fusing the Tshb gene with N-terminal 10xHis tag and C-terminal Myc tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this Tshb protein production. After purification, a premium Tshb recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+.
Tshb is a gene encoding a protein named thyrotropin subunit beta (also commonly calld TSH-B or TSH-beta). The protein encoded by this gene and the α subunit together makes up thyroid stimulating hormone (TSH), which stimulates the thyroid gland to produce thyroxine. Then, triiodothyronine stimulates the metabolism of almost every tissue in the body. In terms of TSH, α subunit is common to pituitary gonadotropins, whereas the β subunit is TSH-specific, providing receptor specificity.