Greater than 90% as determined by SDS-PAGE.
TshbThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain
Rattus norvegicus (Rat)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Full Length of Mature Protein
N-terminal 6xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Recombinant Rat Thyrotropin subunit beta (Tshb) is a partial-length protein expressed with an N-terminal 6xHis-tagged and C-terminal Myc-tagged in the yeast. Its expression region corresponds to 21-132aa of rat Tshb protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band around 18.4 kDa on the gel. This recombinant Tshb protein may be used to synthesize antibodies against Tshb or on the studies of Tshb-associated signal transduction.
Tshb is indispensable for the control of thyroid structure and metabolism. Thyroid stimulating hormone consists of two subunits, alpha and beta. The TSHB gene encodes the beta subunit. The alpha and beta subunits are bound together to produce the active form of the hormone. Mutations in this gene can result in congenital central and secondary hypothyroidism and Hashimoto's thyroiditis.