Code | CSB-RA298469A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
To produce the ACO1 recombinant monoclonal antibody, a multi-step procedure is followed. Initially, the ACO1 monoclonal antibody is harvested and its gene sequence is analyzed. An ACO1 monoclonal antibody gene-carrying vector is then constructed and introduced into a host cell line for culture. A synthesized peptide, derived from human ACO1, is used as the immunogen during ACO1 monoclonal antibody production. The ACO1 recombinant monoclonal antibody is purified through affinity chromatography from the cell culture supernatant, and its specificity is confirmed using ELISA, WB, IF, and FC applications. It is exclusively reactive with human ACO1 protein.
ACO1 (Aconitase 1) is an iron-sulfur protein that plays an essential role in the Krebs cycle, which is the metabolic pathway that generates ATP molecules to supply energy to cells. ACO1 is responsible for converting citrate to isocitrate in the second step of the Krebs cycle, which produces NADH and CO2 as byproducts. In addition to its role in energy metabolism, ACO1 has also been shown to play a role in regulating iron homeostasis and responding to oxidative stress in cells.
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