ATF4 Antibody

Code CSB-RA002272A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
    All lanes: ATF4 antibody at 1.6μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39 KDa
    Observed band size: 50 KDa

  • IHC image of CSB-RA002272A0HU diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-RA002272A0HU at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitating ATF4 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA002272A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA002272A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)

  • Overlay histogram showing Hela cells stained with CSB-RA002272A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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Product Details

Uniprot No. P18848
Target Names ATF4
Alternative Names Activating transcription factor 4 antibody; ATF 4 antibody; ATF4 antibody; ATF4 protein antibody; ATF4_HUMAN antibody; cAMP-dependent transcription factor ATF-4 antibody; cAMP-responsive element-binding protein 2 antibody; CREB 2 antibody; CREB-2 antibody; CREB2 antibody; Cyclic AMP dependent transcription factor ATF 4 antibody; Cyclic AMP response element binding protein 2 antibody; Cyclic AMP-dependent transcription factor ATF-4 antibody; Cyclic AMP-responsive element-binding protein 2 antibody; DNA binding protein TAXREB67 antibody; DNA-binding protein TAXREB67 antibody; Tax Responsive Enhancer Element B67 antibody; Tax-responsive enhancer element-binding protein 67 antibody; TaxREB67 antibody; TXREB antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from human ATF4
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IF, FC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
IP 1:200-1:1000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional activator. Binds the cAMP response element (CRE) (consensus
Gene References into Functions
  1. phosphorylated PERK and ATF4 would be upregulated in Orexin neurons in Sudden Infant Death Syndrome (SIDS) compared to non-SIDS. PMID: 27796753
  2. Our data suggests a novel interaction between Nrf2 and ATF4 under oxidative and endoplasmic reticulum stress, thus drives specific enzymatic and non-enzymatic reactions of antioxidant mechanisms maintaining redox homeostasis. PMID: 29421327
  3. PSAT1, which is overexpressed in ER-negative breast cancers, is activated by ATF4 and promotes cell cycle progression via regulation of the GSK3beta/beta-catenin/cyclin D1 pathway. PMID: 29216929
  4. POSTN may function as a protective factor for osteoblasts during this process by inhibiting the eIF2alphaATF4 pathway. PMID: 29207036
  5. p62 directly targets nuclear transcription factors to control metabolic reprogramming in the microenvironment and repress tumorigenesis, and identifies ATF4 as a synthetic vulnerability in p62-deficient tumor stroma. PMID: 28988820
  6. results suggest a conditional regulation of KRT16 gene by ATF4 that may be inhibited in normal cells, but engaged during cancer progression. Potential roles of KRT16, FAM129A and HKDC1 genes upregulation in adaptive stress responses and pathologies are discussed PMID: 29420561
  7. Results provide evidence that the availability of glucose controls ATF4-mediated MITF suppression to drive melanoma cell proliferation. PMID: 28380427
  8. Decreased ATF4 expression as a mechanism of acquired resistance to long-term amino acid limitation in cancer cells PMID: 28460466
  9. These results suggest that p21 induction plays a vital role in the cellular response to ER stress and indicate that p21 is a prosurvival effector of ATF4. PMID: 28975618
  10. GRP78 inhibition enhances ATF4-induced cell death by the deubiquitination and stabilization of CHOP in human osteosarcoma cells. PMID: 28947141
  11. Expression of either dominant-negative or constitutively active mutants of Nrf2, ATF4, or c-Jun confirmed that distinct transcription units are regulated by these transcription factors. PMID: 27278863
  12. ATF4 contributes to tumor growth of endometrial cancer (EC) by promoting CCL2 and subsequent recruitment of macrophage, and ATF4/CCL2 axis might be a potential therapeutic target for EC. PMID: 28843961
  13. ATF4 expression fosters the malignancy of primary brain tumors and increases proliferation and tumor angiogenesis; experiments revealed that ATF4-dependent tumor promoting effects are mediated by transcriptional targeting the glutamate antiporter xCT PMID: 28553953
  14. The PERK-eIF2alpha-ATF4-CHOP signaling pathway has a critical role in tumor progression during endoplasmic reticulum stress. (Review) PMID: 27211800
  15. ATF4 pathway is activated in vivo upon mitochondrial stress. PMID: 28566324
  16. a shortage of tryptophan caused by expression of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) resulted in ATF4-dependent upregulation of several amino acid transporters. PMID: 27651314
  17. SLC30A10 has a protective role in 1-methyl-4-phenylpyridinium-induced toxicity via PERK-ATF4 pathway. PMID: 28688763
  18. There was decreased or loss of ATF4 in 52% of medullary thyroid cancer (MTC) tumors (n = 39) compared with normal thyroid follicle cells. A negative correlation was observed between RET and ATF4 protein levels in MTC tumors. PMID: 27935748
  19. hypoxia-induced ATF4 expression may promote progression of proliferating infantile hemangioma through macrophage colony-stimulating factor induced M2-polarized macrophages infiltration. PMID: 28438094
  20. overexpression of eIF5 and 5MP induces translation of ATF4. PMID: 27325740
  21. ATF4 may exert various physiological roles in lipid metabolism depending on the nutrient composition. In addition, these results suggested that ATF4 has a role in regulating lipogenesis and in the development of NAFLD; thus ATF4 may be considered a therapeutic target for NAFLD. PMID: 27357269
  22. PERK-eIF2alpha-ATF4 signaling pathway mediated by endoplasmic reticulum stress involved in osteoblast differentiation of periodontal ligament cells under cyclic mechanical force. PMID: 27079961
  23. The localization of ATF4 in the granular component of nucleoli together with its association with nascent RNA transcripts in cells undergoing proteotoxic cell stress could suggest a new function for ATF4 in cell stress management. PMID: 27567537
  24. The results showed that the FGF21 promoter contains three response elements for ATF4, suggesting that FGF21 is a sensitive target of ATF4. PMID: 27010621
  25. ADM-2 is a stress-inducible gene controlled by ATF-4. PMID: 27328454
  26. The results suggest that C12orf39, CSTA, and CALCB are novel ATF4 target genes, and that C12orf39 promoter activity is activated by ATF4 through amino acid response element. PMID: 26967115
  27. High ATF4 expression is associated with osteosarcoma progression. PMID: 26797758
  28. miR-214 directly targeted ATF4, a crucial transcriptional factor involved in anti-stress responses, down regulation of miR-214 releases the repression of ATF4 translation and leads to increased ATF4 protein content. PMID: 26791102
  29. The activation of ATF4 in response to ONC201 required the kinases HRI and PKR, which phosphorylate and activate the translation initiation factor eIF2alpha. PMID: 26884600
  30. TBL2 participates in ATF4 translation through its association with the mRNA. PMID: 26239904
  31. Inhibition or overexpression of ATF4 confirms the role of ATF4 in SESN2 gene up-regulation induced by mitochondrial dysfunction. PMID: 26771712
  32. ATF4 and ATF6beta act synergistically in the negative regulation of placental growth factor mRNA expression PMID: 26648175
  33. Authors observed that a slow rate of ATF4-translation and late re-initiation of general translation coincided with cells which were resistant to ER stress-induced cell death. PMID: 25633195
  34. a reduction of cell death was associated with decreased levels of ATF4 in a rhabdomyosarcoma cell line PMID: 26172539
  35. Combined administration inhibited the cells most potently and time-dependently, decreased the expression of HO-1, and significantly increased the expression of ATF4, CHOP, and Ire-1 proteins expression levels PMID: 26125799
  36. Global profiling in human mesenchymal stem cells and a novel cell-free assay reveals that ATF4 requires C/EBPbeta for genomic binding at a motif distinct from that bound by the C/EBPbeta homodimer. PMID: 26111340
  37. This study outlines the mechanism of NIR laser phototoxicity and the utility of monitoring surface temperature and ATF4 expression as potential biomarkers to develop safe and effective clinical applications. PMID: 26030745
  38. Up-regulation of ATF4 is associated with Pancreatic Neuroendocrine Tumors. PMID: 26504039
  39. The ATF4/p75NTR/IL-8 signal pathway may have an important role in EndoMT induced by SFO. PMID: 24905361
  40. ATF4 is a potential biomarker for esophageal squamous cell carcinoma (ESCC) prognosis and its dysregulation may play a key role in the regulation of invasion and metastasis in ESCC. PMID: 25078779
  41. Upon loss of attachment in tumor cells, ATF4 activated a program of cytoprotective autophagy and antioxidant responses, including induced expression of heme oxygenase 1 (HO-1). Increased levels of HO-1 ameliorated oxidative stress and cell death. PMID: 26011642
  42. Treatment with a skin sensitizer rapidly induces the phosphorylation of eIF2a and a concomitant increase of ATF4 protein levels in dendritic cells. PMID: 25236743
  43. The results demonstrate that the endoplasmic reticulum stress-regulated ATF4/p16 pathway is involved in the premature senescence of renal tubular epithelial cells during diabetic nephropathy progression. PMID: 25567807
  44. RET as a novel dual kinase with nuclear localization and provide mechanisms by which RET represses the proapoptotic genes PMID: 25795775
  45. A sustained deficiency of mitochondrial respiratory complex III induces an apoptotic cell death through the p53-mediated inhibition of pro-survival activities of the ATF4. PMID: 25375376
  46. ATF4 signaling pathway is essential for mediating the effect of ER stress on beta-klotho expression. PMID: 25727012
  47. B-cell lymphoma/leukemia 10 promotes oral cancer progression through STAT1/ATF4/S100P signaling pathway. PMID: 24681956
  48. ATF4-mediated repression of apelin contributes substantially to the pro-apoptotic effects of p38. PMID: 25052841
  49. Bone diseases of diabetes mellitus type 2 showing definite changes of ATP4 gene expression. PMID: 24715035
  50. the PERK/ATF4/LAMP3-arm of the UPR is an additional pathway mediating hypoxia-induced breast cancer cell migration PMID: 23294542
  51. ASCT2 expression, which correlates with that of N-Myc and ATF4, is markedly elevated in high-stage neuroblastoma PMID: 25142020
  52. High ATF4 expression is associated with trastuzumab-resistant breast cancer. PMID: 25449779
  53. Then, activated p38 further enhanced the docking of activating transcription factor 4 (ATF4) onto the CHOP (CCAAT/enhancer-binding protein homologous protein) promoter via eIF2alpha to enhance apoptosis PMID: 24874742
  54. These results reveal an active role for intra-axonal translation in neurodegeneration and identify ATF4 as a mediator for the spread of Alzheimer's disease pathology. PMID: 25171414
  55. ATF4 may promote multidrug resistance in esophageal squamous cell carcinoma cells through the up-regulation of STAT3 expression PMID: 25130172
  56. Results show that Nrf2 and ATF4 were upregulated by proteasome inhibition and cooperatively enhance human xCT gene expression upon proteasome inhibition. PMID: 25002527
  57. Endoplasmic reticulum stress could promote cell apoptosis through the upregulation of ATF4 levels PMID: 24308964
  58. In the current study, authors showed that the NAD(+)-dependent deacetylase, SIRT1, suppresses ATF4 synthesis during proteasome inhibition. PMID: 24100623
  59. Anacardic acid induces HepG2 tumor cell apoptosis associated with ATF4-dependent endoplasmic reticulum stress. PMID: 24853302
  60. results show that ATF4-regulated SESN2 expression presents a new link between ER stress and mTOR inhibition and autophagy. PMID: 23916134
  61. ATF4 silencing prevented the activating effect of histidinol and tunicamycin on ATF4 and ALT2 expression. Our findings point to ALT2 as an enzyme involved in the metabolic adaptation of the cell to stress PMID: 24418603
  62. Lower expression of ATF4 and VEGF genes in chorionic villus tissue may participate in the pathogenesis of spontaneous abortion. PMID: 23891064
  63. These results indicate that increased expression of p-ATF4 (ser 245) may contribute to cancer development of non-small cell lung cancer and may be a potential cancer marker. PMID: 23975372
  64. Data indicate that suppressing GCN2 and activating transcription factor 4 (ATF4), expression decreased Amino acid deprivation (AAD)-induced VEGF expression. PMID: 23908598
  65. ATF4 is involved in ER stress signalling through the AARE, which further supports our finding that CK2 inhibition provokes an amino acid induced response pathway. PMID: 22609407
  66. different species of OxPLs, which are present in the human eye are capable of stimulating expression of VEGF in fetal and primary RPE cells via ATF4-dependent mechanisms. PMID: 24021586
  67. Upregulated ATF4 results in the transcriptional initiation of the apoptosis-related chop gene and autophagy-related map1lc3b gene, through which selenite links ER stress to apoptosis and autophagy during leukemia treatment PMID: 23792164
  68. Signaling dynamics of palmitate-induced ER stress responses mediated by ATF4 in HepG2 cells. PMID: 23339444
  69. This study demonstrates a molecular "field of injury " throughout the bronchial airway of active and former smokers with COPD that may be driven in part by ARF4. PMID: 23471465
  70. ATF4 acts as a positive regulator in TLR4-triggered cytokine production. PMID: 23241898
  71. These results show for the first time that ATF4 induction might have an important role in HIV replication, and suggest that ATF4 might represent a convergent signaling molecule for different stressors important in regulating the HIV-1 cycle. PMID: 22050711
  72. ATF4 inhibit DNA repair protein DNA-dependent protein kinase 1 induced by heat shock PMID: 23001845
  73. ATF4 increases resistance to cisplatin in the hepatocellular carcinoma by increased biosynthesis of glutathione PMID: 22338651
  74. Identification of a novel ATF4 binding site within the EPO 3'-enhancer region, suggests a distinct role for ATF4 in the unfolded protein response-dependent suppression of the enhancer. PMID: 23242184
  75. The BH3-only protein harakiri (HRK) is transactivated by ATF4 in severe hypoxia through direct binding of ATF4 to the promoter region. PMID: 23090478
  76. glucose deprivation enhanced F7 expression in a mechanism reliant on prior ATF4 upregulation primarily due to increased transcription from the ATF4 gene. PMID: 22848420
  77. ATF4 plays a protective role in Parkinson's disease through the regulation of parkin. PMID: 23392669
  78. Data show that the autophagy-initiating kinase ULK1 (UNC51-like kinase 1) is a direct transcriptional target of ATF4 (activating transcription factor 4), which drives the expression of ULK1 mRNA and protein in severe hypoxia and ER stress. PMID: 23078367
  79. Overexpression of ATF4 in Caco-2 cells induced increased FGF19 mRNA expression, whereas shRNA (short hairpin RNA)-mediated depletion of ATF4 PMID: 23205607
  80. PERK plays a critical role in information processing and cognitive function and that modulation of eIF2alpha phosphorylation and ATF4 expression. PMID: 22813743
  81. ATF4 and Nrf2 can control CHOP induction in thyroid cancer cells by proteasome inhibition. PMID: 22691366
  82. Glutamine depletion in MYC-transformed cells induces apoptosis through ATF4-dependent, but p53-independent, PUMA and NOXA induction. PMID: 23153536
  83. results show that the PERK/ATF4 arm of UPR mediates the angiogenic switch and is a potential target for antiangiogenic cancer therapy. PMID: 22915762
  84. ATF4-dependent regulation of the JMJD3 gene during amino acid deprivation can be rescued in Atf4-deficient cells by inhibition of deacetylation. PMID: 22955275
  85. Data show that Disrupted-In-Schizophrenia 1 (DISC1) interacts with the transcription factor activating transcription factor 4 (ATF4) in the nucleus, and the common variant 607F additionally reduces DISC1/ATF4 interaction. PMID: 22422769
  86. Saturated fatty acid-induced ER stress and apoptosis in these human liver cells were enacted through the PERK/ATF4/CHOP signaling pathway. PMID: 22246806
  87. Changes in the bioenergetic function of mitochondria are mimicked by activation of AMP kinase and silencing of ATF4. PMID: 22435535
  88. ATF4 facilitated multidrug resistance in gastric cancer cells through direct binding to the SIRT1 promoter. PMID: 22363646
  89. Twist1 overexpression in osteoblasts attenuates ATF4 binding to the osteocalcin promoter in response to Parathyroid hormone. PMID: 21866569
  90. These results add FGF21 gene induction to the transcriptional programme initiated by increased levels of ATF4 and offer a new mechanism for the induction of the FGF21 gene expression under nutrient deprivation PMID: 22233381
  91. elevated expression of p-CREB-2 (ser 245) may potentially contribute to carcinogenesis and cancer development of breast carcinoma PMID: 22052162
  92. Coexistence of PHD1 stabilized ATF4, as opposed to the destabilization of ATF4 by PHD3. PMID: 21951999
  93. HHV-8 upregulates ATF4 expression and induces MCP-1 production and proangiogenic properties in endothelial cells. PMID: 22016052
  94. ATF4 protein binds and activates the AARE regulatory sites of the CHOP promoter. ORF2 expression also leads to increased phosphorylation of eIF2alpha that in turn initiates the translation of ATF4 mRNA. PMID: 21966512
  95. [review] First evidence of a functional interaction between ATF4, FIAT (factor-inhibiting ATF4-mediated transcription) and alphaNAC (nascent polypeptide-associated complex and coactivator alpha). PMID: 22082360
  96. we show that RPL41 induces ATF4 phosphorylation and rapid degradation in the proteasome PMID: 21706477
  97. stress-induced upregulation of parkin is mediated by activating transcription factor 4 PMID: 21113145
  98. DKK1 is a novel target gene in the response to amino acid deficiency and that the expression of DKK1 is up-regulated through an ATF4-independent and an ERK-dependent pathway. PMID: 21683813
  99. ATF4 binding to the COX2 promoter plays a critical role in HBx-mediated COX2 induction. PMID: 21244365
  100. the GCN2/eIF2alpha/ATF4 pathway is essential for the induction of the TRB3 gene transcription PMID: 21203563

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Subcellular Location Cytoplasm, Cell membrane, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Protein Families BZIP family
Database Links

HGNC: 786

OMIM: 604064

KEGG: hsa:468

STRING: 9606.ENSP00000336790

UniGene: Hs.496487

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