DNAJA1 Antibody

Code CSB-RA589492A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
    All lanes: DNAJA1 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 45, 38 kDa
    Observed band size: 45-55 kDa
  • Immunofluorescence staining of A549 cell with CSB-RA589492A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 550-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing A549 cells stained with CSB-RA589492A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No. P31689
Target Names DNAJA1
Alternative Names DnaJ homolog subfamily A member 1 (DnaJ protein homolog 2) (HSDJ) (Heat shock 40 kDa protein 4) (Heat shock protein J2) (HSJ-2) (Human DnaJ protein 2) (hDj-2), DNAJA1, DNAJ2 HDJ2 HSJ2 HSPF4
Species Reactivity Human
Immunogen A synthesized peptide derived from human DNAJA1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 23C6
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IF 1:50-1:200
FC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
Gene References into Functions
  1. that DNAJA1 controls the fate of misfolded stabilization of mutant p53 PMID: 27775703
  2. Therefore, we propose that DnaJA1 is co-opted by the influenza A virus to enter the nucleus and to enhance its RNA polymerase activity in an Hsp70 cochaperone-independent manner. PMID: 25253355
  3. structure and function of human DNAJA1 and its relationship to pancreatic cancer PMID: 24512202
  4. we combined the Hsp70-NEF pairs with cochaperones of the J protein family (DnaJA1, DnaJA2, DnaJB1, and DnaJB4) to generate 16 permutations. PMID: 24318877
  5. Moreover, the levels of DnaJA1 and Hsp70 seem to play against each other with regard to tau: as DnaJA1 levels increase, tau levels are reduced, but this can be prevented if Hsp70 levels are simultaneously induced. PMID: 22343013
  6. Hdj2 directly associates with Japanese encephalitis virus nonstructural protein NS5 and facilitates viral replication. PMID: 21999493
  7. Hsc70 and a dimer of DjA1 independently bind to an unfolded protein PMID: 20363747
  8. Hsp40 type 1 chaperones DJA1 (DNAJA1/Hdj2) and DJA2 (DNAJA2) as key modulators of hERG degradation PMID: 19940115
  9. Mammalian, yeast, bacterial, and chemical chaperones reduce aggregate formation and death in a cell model of oculopharyngeal muscular dystrophy PMID: 11796717
  10. HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor PMID: 11809754
  11. sequence analysis of two isoforms PMID: 12974469
  12. Multiple tissue polymerase chain reaction (PCR) results showed that nDnaJA1 expressed highly in testis and lung but low in thymus, prostate, colon and liver PMID: 15595953
  13. Results indicate that DjA1 and DjB4 of subfamilies A and B of human Hsp40 have different quaternary structures and chaperone functions. PMID: 15661747
  14. DJA1 was inhibitory of refolding with DJA2 and Hsc70. PMID: 18684711

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Subcellular Location Membrane; Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm, perinuclear region. Mitochondrion.
Tissue Specificity Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Database Links

HGNC: 5229

OMIM: 602837

KEGG: hsa:3301

STRING: 9606.ENSP00000369127

UniGene: Hs.445203

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