DNAJA1 Antibody

Code CSB-PA006999LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate
    All lanes: DNAJA1 antibody at 3.4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 45, 38 kDa
    Observed band size: 45, 38 kDa

  • IHC image of CSB-PA006999LA01HU diluted at 1:1000 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA006999LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) DNAJA1 Polyclonal antibody
Uniprot No. P31689
Target Names DNAJA1
Alternative Names DJ 2 antibody; DJ2 antibody; DjA1 antibody; DnaJ (Hsp40) homolog; subfamily A; member 1 antibody; DnaJ homolog subfamily A member 1 antibody; DnaJ protein homolog 2 antibody; DNAJ2 antibody; Dnaja1 antibody; DNJA1_HUMAN antibody; hDJ 2 antibody; HDJ-2 antibody; HDJ2 antibody; Heat shock 40 kDa protein 4 antibody; heat shock protein DNAJ like 2 antibody; Heat shock protein J2 antibody; HSDJ antibody; HSJ-2 antibody; HSJ2 antibody; HSPF4 antibody; Human DnaJ protein 2 antibody; NEDD7 antibody; Neural precursor cell expressed developmentally down regulated 7 antibody; OTTHUMP00000021193 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human DnaJ homolog subfamily A member 1 protein (8-106AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The DNAJA1 Antibody (Product code: CSB-PA006999LA01HU) is Non-conjugated. For DNAJA1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA006999LB01HU DNAJA1 Antibody, HRP conjugated ELISA
FITC CSB-PA006999LC01HU DNAJA1 Antibody, FITC conjugated
Biotin CSB-PA006999LD01HU DNAJA1 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:1000-1:2000
IF 1:200-1:500
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
Gene References into Functions
  1. that DNAJA1 controls the fate of misfolded stabilization of mutant p53 PMID: 27775703
  2. Therefore, we propose that DnaJA1 is co-opted by the influenza A virus to enter the nucleus and to enhance its RNA polymerase activity in an Hsp70 cochaperone-independent manner. PMID: 25253355
  3. structure and function of human DNAJA1 and its relationship to pancreatic cancer PMID: 24512202
  4. we combined the Hsp70-NEF pairs with cochaperones of the J protein family (DnaJA1, DnaJA2, DnaJB1, and DnaJB4) to generate 16 permutations. PMID: 24318877
  5. Moreover, the levels of DnaJA1 and Hsp70 seem to play against each other with regard to tau: as DnaJA1 levels increase, tau levels are reduced, but this can be prevented if Hsp70 levels are simultaneously induced. PMID: 22343013
  6. Hdj2 directly associates with Japanese encephalitis virus nonstructural protein NS5 and facilitates viral replication. PMID: 21999493
  7. Hsc70 and a dimer of DjA1 independently bind to an unfolded protein PMID: 20363747
  8. Hsp40 type 1 chaperones DJA1 (DNAJA1/Hdj2) and DJA2 (DNAJA2) as key modulators of hERG degradation PMID: 19940115
  9. Mammalian, yeast, bacterial, and chemical chaperones reduce aggregate formation and death in a cell model of oculopharyngeal muscular dystrophy PMID: 11796717
  10. HSP40 binding is the first step in the HSP90 chaperoning pathway for the progesterone receptor PMID: 11809754
  11. sequence analysis of two isoforms PMID: 12974469
  12. Multiple tissue polymerase chain reaction (PCR) results showed that nDnaJA1 expressed highly in testis and lung but low in thymus, prostate, colon and liver PMID: 15595953
  13. Results indicate that DjA1 and DjB4 of subfamilies A and B of human Hsp40 have different quaternary structures and chaperone functions. PMID: 15661747
  14. DJA1 was inhibitory of refolding with DJA2 and Hsc70. PMID: 18684711

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Subcellular Location Membrane; Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm, perinuclear region. Mitochondrion.
Tissue Specificity Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Database Links

HGNC: 5229

OMIM: 602837

KEGG: hsa:3301

STRING: 9606.ENSP00000369127

UniGene: Hs.445203

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