His-Tag Monoclonal Antibody

Code CSB-MA000159
Size US$100
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  • IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with His-tag fusion protein
    Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
    Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
    Lane 6: Recombinant protein (E.coli)

  • IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)

  • 2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

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Product Details

Target Names
His-Tag
Raised in
Mouse
Species Reactivity
N/A
Immunogen
Synthetic Peptide
Conjugate
Non-conjugated
Isotype
IgG
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
His-Tag monoclonal antibody CSB-MA000159 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The immunogen is a short peptide. It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This His-Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. An efficient detection and purification system based on fusion proteins can be established using the His tag. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 4 reviews

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Applications : Pull-down assay

Review: Competitive binding assays of CgRuby1 and CgRuby2Short binding to CgbHLH1. The mixture of HIS-CgRuby1 and FLAG CgRuby2Short was added to immobilized GSTCgbHLH1. The precipitates were detected using western blot analysis with anti-HIS, anti-FLAG or anti-GST antibodies. The gradient indicates the increasing amount of FLAG-CgRuby2Short. These experiments were repeated independently twice with similar results.

By Anonymous

Applications : ELISA

Sample dilution: 1: 4000

Review: Murine B16F10 and L929 cell lines (2× 10 5 ) were fixed on plates and then blocked with 2% BSA solution. After that, Rechistatin, Echistatin, LgRec1 or EGFP (1; 3; 6 and 9μM) were added to the plates followed by incubation for 2 h at 37°C. The binding of the recombinant proteins to the cells was assessed using anti-His (Cusabio®) mAb followed by anti-mouse IgG-peroxidase conjugate (Sigma® ). The reaction was measured by spectrophotometer at 492 nm, the higher the absorbance the grater the binding of the toxins to the cells. A) Binding of recombinant toxins on murine B16F10 melanoma cells; B) Binding of recombinant toxins on murine L929 cells. The results were expressed as mean ± SD (n = 3), ***p < 0.001; **p < 0.01 and *p < 0.05 comparing Rechistatin, Echistatin, LgRec1 and EGFP groups com- pared with PBS.

By Anonymous

Applications : GST pull-down

Sample dilution: 1:200

Review: We examined Pit-1 protein binding in relation to ESRRG alternative splicing status. Pit-1 protein fused to glutathione S-transferase (GST) pull-down HIS-tagged cryptic ESRRG, whereas it bound weakly to canonical ESRRG

By Anonymous

Applications : Western blot

Sample type: cells

Review: GST pull-down assay revealed Shank3 directly bound with STIM1 (n = 3, t = 18.65, df = 4, P < 0.0001).

By Anonymous

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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