His-Tag Monoclonal Antibody

Code CSB-MA000159
Size US$167
Image
  • IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
    Lane 1: untransfected 293 cell lysate
    Lane 2: transfected 293 cell lysate with His-tag fusion protein
    Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
    Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
    Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
    Lane 6: Recombinant protein (E.coli)

  • IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)

  • 2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

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Product Details

Target Names His-Tag
Raised in Mouse
Species Reactivity N/A
Immunogen Synthetic Peptide
Conjugate Non-conjugated
Isotype IgG
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form Liquid
Tested Applications ELISA, WB, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Somatic SF3B1 hotspot mutation in prolactinomas. C Li,Nature Communications,2020

Applications: GST pull-down
Antibody dilution factor: 1:200
Review: We examined Pit-1 protein binding in relation to ESRRG alternative splicing status. Pit-1 protein fused to glutathione S-transferase (GST) pull-down HIS-tagged cryptic ESRRG, whereas it bound weakly to canonical ESRRG
PMID: 32427851

When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell. Siqueira RAGB, et al,Toxicon,2019

Applications: ELISA
Antibody dilution factor: 1: 4000
Review: Murine B16F10 and L929 cell lines (2× 10 5 ) were fixed on plates and then blocked with 2% BSA solution. After that, Rechistatin, Echistatin, LgRec1 or EGFP (1; 3; 6 and 9μM) were added to the plates followed by incubation for 2 h at 37°C. The binding of the recombinant proteins to the cells was assessed using anti-His (Cusabio®) mAb followed by anti-mouse IgG-peroxidase conjugate (Sigma® ). The reaction was measured by spectrophotometer at 492 nm, the higher the absorbance the grater the binding of the toxins to the cells. A) Binding of recombinant toxins on murine B16F10 melanoma cells; B) Binding of recombinant toxins on murine L929 cells. The results were expressed as mean ± SD (n = 3), ***p < 0.001; **p < 0.01 and *p < 0.05 comparing Rechistatin, Echistatin, LgRec1 and EGFP groups com- pared with PBS.
PMID: 31251993

Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus. Huang D, et al,Nature Plants,2018

Application: Pull-down assay
Review: Competitive binding assays of CgRuby1 and CgRuby2Short binding to CgbHLH1. The mixture of HIS-CgRuby1 and FLAG CgRuby2Short was added to immobilized GSTCgbHLH1. The precipitates were detected using western blot analysis with anti-HIS, anti-FLAG or anti-GST antibodies. The gradient indicates the increasing amount of FLAG-CgRuby2Short. These experiments were repeated independently twice with similar results.
PMID: 30374094

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