Name: Recombinant Human High affinity immunoglobulin gamma Fc receptor I (FCGR1A)
Brand: CUSABIO
SKU: CSB-CF008537HU

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

FCGR1A

Uniprot No.

P12314

Research Area

Immunology

Species

Homo sapiens (Human)

Source

in vitro E.coli expression system

Expression Region

16-374aa

Target Protein Sequence

QVDTTKAVITLQPPWVSVFQEETVTLHCEVLHLPGSSSTQWFLNGTATQTSTPSYRITSASVNDSGEYRCQRGLSGRSDPIQLEIHRGWLLLQVSSRVFTEGEPLALRCHAWKDKLVYNVLYYRNGKAFKFFHWNSNLTILKTNISHNGTYHCSGMGKHRYTSAGISVTVKELFPAPVLNASVTSPLLEGNLVTLSCETKLLLQRPGLQLYFSFYMGSKTLRGRNTSSEYQILTARREDSGLYWCEAATEDGNVLKRSPELELQVLGLQLPTPVWFHVLFYLAVGIMFLVNTVLWVTIRKELKRKKKWDLEISLDSGHEKKVISSLQEDRHLEEELKCQEQKEEQLQEGVHRKEPQGAT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

46.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Thanks so much for the information. We are interested in your full length protein CSB-CF008537HU.
I have 3 questions below.
1)Do you know if the full length protein expressed in the cell free system is folded correctly?
2)Does it have Ig binding capacity and if it can be picked up in a western?
3)Can we get a custom synthesis of a larger batch?

Regarding your question, this protein has more glycosylation modifications, but the cell free expression system does not provide post-translational modification, which does not guarantee fold correctly and the structure of the protein. Your experiment only requires Ig binding capacity, if so, we recommend you to choose the complete extracellular domain (CSB-XP008537HU1 series), including Ig-like C2-type. 1-3 Domain, and you'd better choose eukaryotic expression system, most of the literatures select Mammalian cell expression, we recommend CSB-MP008537HU1, you can refer to the literature (https://www.ncbi.nlm.nih.gov/pmc/ Articles/PMC3220521/),but our protein hasn't been verified Ig binding ability, so it is not 100% guaranteed.
Regarding the large quantity, we can provide custom service, but it is recommended to order a small size to have a try before ordering large sizes.
If you have any other questions about it, pls don't be hesitated to contact me.

Thanks so much for your reply. It is very useful indeed! We are not necessarily interested in the Ig binding domain but more in immunological detection of the full length protein in biological samples.
We would like to purchase your ELISA for this and have a few questions that you or someone else can help me with:
1.Would your full length protein you generate be detected in your ELISA CSB-EL008537HU"?
2.What species are the sandwich antibodies and what do they target?

Thanks for your interest on elisa kit #CSB-EL008537HU.
This kit employs the quantitative sandwich enzyme immunoassay technique to detect human FCGR1A.
The capture in this kit is mouse monoclonal antibody and the detection is goat polyclonal antibody.The kit is directed to extracellular domain.Pls refer to https://www.uniprot.org/uniprot/P12314.
As no epitope mapping done on the antibodies in this kit,the specific recognition site is not clear.
This kit can be used to detect natural protein. While we are not sure whether it can be used to detect recombinant protein.
Since the volumn and post-translational modification of the recombinant protein expressed by different expression systems varies.
Also the corresponding antigenicity is also different and we cannot be guaranteed the full length protein we generate can be detected in our ELISA CSB-EL008537HU.
If further questions,pls let me know.I will check accordingly for you.

Target Background

Function

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.

Gene References into Functions

The neutrophil/lymphocyte ratio is a better addition to C-reactive protein than CD64 index as a marker for infection in COPD. PMID: 28185443
FcgammaRI functions as a regulator for immune inflammation via acceleration of NF-kappaB regulating NLRP3 inflammasome signaling PMID: 29920250
HIV infection and HIV replication are associated with up-regulation of neutrophil CD64. PMID: 27221825
Neutrophil CD64 distinguished sepsis and non-septic ICU patients. PMID: 28898742
These results suggest that tyrosine phosphorylation may be critical in FcgammaRI-dependent endocytosis/phagocytosis that may be regulated by LILRB4 by triggering dephosphorylation of key signalling proteins. PMID: 27725776
neutrophil CD64 elevated in septic neonates PMID: 27578316
Neutrophil CD64 expression could be a good predictor as an immune parameter with high sensitivity and a negative predictive value for bacteremia in febrile neutropenic patients. PMID: 27348760
investigated consequences of 3 nonsynonymous SNPs for FcgammaRI; only SNP V39I reduces immune-complex binding of FcgammaRI whereas monomeric IgG binding is unaffected; SNPs I301M and I338T have no influence on monomeric IgG or immune complex binding, but FcRgamma signaling is decreased for both SNPs PMID: 28814603
Studies show that neutrophil CD64 (nCD64) is a reliable biomarker for diagnosing neonatal sepsis. PMID: 28791853
Data revealed that Erythema Nodosum Leprosum neutrophils express CD64, presumably contributing to the immunopathogenesis of the disease. PMID: 27556927
Our findings demonstrate that remarkable CD64 expression during Kawasaki dsease flare-ups may serve as a biomarker for diagnosis. PMID: 27020279
Both activation by Fcgamma-receptor (FcgammaR) dependent and independent stimuli were inhibited by inactive pepsin, indicating inhibition occurs in a downstream signaling pathway used for releasing granules. PMID: 27368805
FCGR1A expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
This study demonstrated that CD64 discriminates between critically ill patients with culture positive and negative sepsis and correlates with severity of disease. However, CD64 index is not a good predictor for 28-day mortality in the critically ill patient. PMID: 27565453
CD64 seems to be a promising marker of infection in the intensive care setting, with Leuko64 showing a slight advantage PMID: 28097142
An elevated neutrophil CD64 index was a reliable prognostic biomarker for both short-term and long-term mortality in patients admitted for acute exacerbation of Chronic obstructive pulmonary disease. PMID: 26922686
Monocyte HLA-DR and neutrophil CD64 expression in critically ill infants with sepsis are related to age and infection. PMID: 26322411
The three-dimensional structure of a human IgG1 Fc fragment bound to wild-type human Fc-gamma RI is reported PMID: 26527150
Combinatorial immunophenotypic analyses showed that loss of PTEN expression with concomitant IGFR-1 expression correlated with poor disease-free survival PMID: 22759273
Early RA patients display increased membrane and soluble CD64 and an impaired FcgammaR function correlating with joint disease activity. PMID: 26406605
CD64 index, CRP and sCD14-ST served as good parameters to determine possible infection in patients that needed intensive care after major procedures. PMID: 25854904
GZMA, GBP5 and CD64 genes show promise as a rapid diagnostic markers separating tuberculosis from other pulmonary diseases. PMID: 26025597
hydrophobic pocket at the surface perfectly suited for residue Leu235 of Fc PMID: 25925696
we address of monocytes functional status through assessment of the patterns of expression of Fcgamma receptors CD64, CD32, CD16 and CD180 receptor on monocytes from CLL patients and healthy individuals using specific mAbs and flow cytometry. PMID: 25802446
FcgammaRI levels were found to not be associated with vascular leakage in dengue patients. PMID: 25566870
The results indicate that conformational plasticity, as well as the openness of the upper CH2 region, is critical for FcgammaR binding and therapeutic effector functions of IgG antibodies. PMID: 26153451
Neutrophil CD64 is a highly sensitive marker for suspected early-onset sepsis in preterm neonates. PMID: 25033045
neutrophil CD64 expression tends to be low in the healthy state and increases in patients with advanced cancer even in the absence of active infection PMID: 26147145
diagnostic accuracy of CD64 index to predict sepsis in critically ill patients PMID: 25324452
Data show that the recombinant Fc gamma receptors FcgammaRIa, FcgammaRIIa, FcgammaRIIb, FcgammaRIIIa(Phe158/Val158), and FcgammaRIIIb glycosylation is complex and varied between receptors. PMID: 25345863
Acute alcohol exposure decreased neutrophil CD64 expression in critically ill nontrauma patients. PMID: 24827394
Data show that IgG4 is capable of transducing signal through FC gamma RI (FcgammaRI). PMID: 25484046
Data indicate that monoclonal antibody Hu 15C1 simultaneously interacts with both Toll like receptor 4 (TLR4) and Fcgamma receptors (FcgammaRI or FcgammaRIIA) at the cell surface. PMID: 25484053
Data suggest that the specific blocking of immunoglobulin G receptor CD64 by H22(scFv) could be used a possible anti-inflammatory mechanism for potentiating the effect of anti-tumor necrosis factor (TNF) antibodies. PMID: 25517313
high expression of CD64 is an indicator important in the diagnosis of infection and sepsis. PMID: 25180450
An elevated neutrophil CD64 index is associated with both mucosal inflammation and an increased risk for clinical relapse in pediatric Crohn disease. PMID: 24788216
CD64 is expressed on neutrophils and monocytes in periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome during flares PMID: 24623459
FCGR1A (high-affinity IgG Fc receptor 1 (CD64)) was the only marker expressed at significantly higher levels in participants with active tuberculosis. PMID: 24205913
Describe biosensing techniques to evaluate the glycosylation pattern of monoclonal antibodies using the extracellular domains of CD64a. PMID: 24810583
CD64 expression on neutrophils increases significantly in neonates with sepsis and is a useful diagnostic marker for early diagnosis of neonatal infection. PMID: 24463977
As a surveillance biomarker, neutrophil CD64 detected LOS/NEC 1.5 days before clinical presentation, but at the expense of performing 41% additional sepsis evaluations PMID: 24046202
Neutrophil CD64 (nCD64) expression was higher in patients with severe sepsis/septic shock (81.2%) and sepsis(78.8%) as compared to those with traumatic brain injury (5.5%) or controls (0.9%, p < 0.0001). PMID: 23452299
FcgammaRI (CD64) promotes IgG-mediated inflammation, anaphylaxis, and antitumor immunotherapy. PMID: 23293080
Screening identified 19 amino acid mutations contributing to the thermal stability and production rate of rhFcgammaRI PMID: 22967788
Data indicate a tool for measuring antibodies triggering activation of FcgammaRIIIA, FcgammaRIIA or FcgammaRI receptors. PMID: 23023090
Simultaneous quantitative analysis of CD64 and CD35 expression on neutrophils might be useful to distinguish between bacterial and viral infections in rheumatoid arthritis patients. PMID: 22237765
observations suggest that serum amyloid P, at least in part, uses FcgammaRI and FcRgamma to inhibit fibrocyte differentiation PMID: 22493081
Data indicate the simultaneous analysis of CD64 together with CD304 (Neuropilin-1) or the combination of CD11b and CD38 was suitable for the identification of rheumatoid arthritis (RA)patients with high current activity in synovitis. PMID: 22251373
Neutrophil CD64 appears to be a better diagnostic test than traditional hematological assays. PMID: 22420242
Expression of CD64 (FcgammaRI) in skin of patients with acute GVHD. PMID: 21243031

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.

Protein Families

Immunoglobulin superfamily, FCGR1 family

Tissue Specificity

Monocyte/macrophage specific.

Database Links

HGNC: 3613

OMIM: 146760

KEGG: hsa:2209

STRING: 9606.ENSP00000358165

UniGene: Hs.534956

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