Human High affinity immunoglobulin gamma Fc receptor I(FCGR1A) ELISA kit

Code CSB-EL008537HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
Fc fragment of IgG, high affinity Ia, receptor (CD64)
Alternative Names
FCGR1A; FCG1; FCGR1; IGFR1; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRIa; CD antigen CD64
Abbreviation
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.2 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FCGR1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 95    
Range % 90-100    
1:2 Average % 88    
Range % 84-92    
1:4 Average % 97    
Range % 92-101    
1:8 Average % 102    
Range % 98-107    
Recovery
The recovery of human FCGR1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 84 80-88    
EDTA plasma (n=4) 96 91-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
50 2.671 2.515 2.593 2.443    
25 2.004 1.973 1.989 1.839    
12.5 1.439 1.484 1.462 1.312    
6.25 0.927 0.942 0.935 0.785    
3.12 0.513 0.527 0.520 0.370    
1.56 0.358 0.369 0.364 0.214    
0.78 0.262 0.251 0.257 0.107    
0 0.151 0.148 0.150      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human FCGR1A ELISA Kit was designed for the quantitative measurement of Human FCGR1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.2 ng/mL.

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 Q&A
Q:

Please provide the following information regarding Human High affinity immunoglobulin gamma Fc receptor I, FCGR1A ELISA Kit (CSB-EL008537HU):
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. What is the immunogen of the capture antibody?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen of the detection antibody?
9.What samples have been tested? Reference values?

A:
Thanks for your inquiry! CSB-EL008537HU
This kit is designed according to extracellular domain.
Capture antibody is mouse monoclonal antibody and immunogen is NS0-derived recombinant protein.( full length).
Detection antibody is goat polyclonal antibody and immunogen is NS0-derived recombinant protein. (fragment).
Standard is NS0-derived recombinant protein,sorry we can not disclose the sequence.
We tested serum and plasma before and the test value is 0.8-3ng/ml. Some individual samples can not be detected out.
Q:

We are looking for a ELISA kit that can detect CD64 from whole blood. This product could be of great use for our project. I have been looking for more details about the capture and detector antibodies that has been used in this ELISA. Unfortunately, I could not find much of information about that. Can you please let us know anything about the following queries
1. What type of detection and capture antibody has been used in this ELISA kit?
2. Do you sell the antibody pair in a solution rather than as a ELISA kit?
3. Can we get a sample to test them in our lab before ordering? In this regard, I want to let you know that it is a three years project which has just been started. If it serves our purpose, we will go for a bulk order.

A:
Thanks for your inquiry!
CD64 has 3 homologous genes: CD64A、CD64B and CD64C. They can be encoded to form 6 different mRNA transcripts.
Pls refer to: https://max.book118.com/html/2015/0613/18934425.shtm.
The study about 6 transcripts have shown that A gene coding has a higher affinity.
Pls refer to: https://www.ncbi.nlm.nih.gov/pubmed/9881690.
So usually CD64 is regarded as FCGR1A.
CSB-EL008537HU
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Sorry we can not sell the antibodies in the kit separately.
We can sell a 24T sample kit at $150 for you to have a try.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Gene References into Functions
  1. The neutrophil/lymphocyte ratio is a better addition to C-reactive protein than CD64 index as a marker for infection in COPD. PMID: 28185443
  2. FcgammaRI functions as a regulator for immune inflammation via acceleration of NF-kappaB regulating NLRP3 inflammasome signaling PMID: 29920250
  3. HIV infection and HIV replication are associated with up-regulation of neutrophil CD64. PMID: 27221825
  4. Neutrophil CD64 distinguished sepsis and non-septic ICU patients. PMID: 28898742
  5. These results suggest that tyrosine phosphorylation may be critical in FcgammaRI-dependent endocytosis/phagocytosis that may be regulated by LILRB4 by triggering dephosphorylation of key signalling proteins. PMID: 27725776
  6. neutrophil CD64 elevated in septic neonates PMID: 27578316
  7. Neutrophil CD64 expression could be a good predictor as an immune parameter with high sensitivity and a negative predictive value for bacteremia in febrile neutropenic patients. PMID: 27348760
  8. investigated consequences of 3 nonsynonymous SNPs for FcgammaRI; only SNP V39I reduces immune-complex binding of FcgammaRI whereas monomeric IgG binding is unaffected; SNPs I301M and I338T have no influence on monomeric IgG or immune complex binding, but FcRgamma signaling is decreased for both SNPs PMID: 28814603
  9. Studies show that neutrophil CD64 (nCD64) is a reliable biomarker for diagnosing neonatal sepsis. PMID: 28791853
  10. Data revealed that Erythema Nodosum Leprosum neutrophils express CD64, presumably contributing to the immunopathogenesis of the disease. PMID: 27556927
  11. Our findings demonstrate that remarkable CD64 expression during Kawasaki dsease flare-ups may serve as a biomarker for diagnosis. PMID: 27020279
  12. Both activation by Fcgamma-receptor (FcgammaR) dependent and independent stimuli were inhibited by inactive pepsin, indicating inhibition occurs in a downstream signaling pathway used for releasing granules. PMID: 27368805
  13. FCGR1A expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  14. This study demonstrated that CD64 discriminates between critically ill patients with culture positive and negative sepsis and correlates with severity of disease. However, CD64 index is not a good predictor for 28-day mortality in the critically ill patient. PMID: 27565453
  15. CD64 seems to be a promising marker of infection in the intensive care setting, with Leuko64 showing a slight advantage PMID: 28097142
  16. An elevated neutrophil CD64 index was a reliable prognostic biomarker for both short-term and long-term mortality in patients admitted for acute exacerbation of Chronic obstructive pulmonary disease. PMID: 26922686
  17. Monocyte HLA-DR and neutrophil CD64 expression in critically ill infants with sepsis are related to age and infection. PMID: 26322411
  18. The three-dimensional structure of a human IgG1 Fc fragment bound to wild-type human Fc-gamma RI is reported PMID: 26527150
  19. Combinatorial immunophenotypic analyses showed that loss of PTEN expression with concomitant IGFR-1 expression correlated with poor disease-free survival PMID: 22759273
  20. Early RA patients display increased membrane and soluble CD64 and an impaired FcgammaR function correlating with joint disease activity. PMID: 26406605
  21. CD64 index, CRP and sCD14-ST served as good parameters to determine possible infection in patients that needed intensive care after major procedures. PMID: 25854904
  22. GZMA, GBP5 and CD64 genes show promise as a rapid diagnostic markers separating tuberculosis from other pulmonary diseases. PMID: 26025597
  23. hydrophobic pocket at the surface perfectly suited for residue Leu235 of Fc PMID: 25925696
  24. we address of monocytes functional status through assessment of the patterns of expression of Fcgamma receptors CD64, CD32, CD16 and CD180 receptor on monocytes from CLL patients and healthy individuals using specific mAbs and flow cytometry. PMID: 25802446
  25. FcgammaRI levels were found to not be associated with vascular leakage in dengue patients. PMID: 25566870
  26. The results indicate that conformational plasticity, as well as the openness of the upper CH2 region, is critical for FcgammaR binding and therapeutic effector functions of IgG antibodies. PMID: 26153451
  27. Neutrophil CD64 is a highly sensitive marker for suspected early-onset sepsis in preterm neonates. PMID: 25033045
  28. neutrophil CD64 expression tends to be low in the healthy state and increases in patients with advanced cancer even in the absence of active infection PMID: 26147145
  29. diagnostic accuracy of CD64 index to predict sepsis in critically ill patients PMID: 25324452
  30. Data show that the recombinant Fc gamma receptors FcgammaRIa, FcgammaRIIa, FcgammaRIIb, FcgammaRIIIa(Phe158/Val158), and FcgammaRIIIb glycosylation is complex and varied between receptors. PMID: 25345863
  31. Acute alcohol exposure decreased neutrophil CD64 expression in critically ill nontrauma patients. PMID: 24827394
  32. Data show that IgG4 is capable of transducing signal through FC gamma RI (FcgammaRI). PMID: 25484046
  33. Data indicate that monoclonal antibody Hu 15C1 simultaneously interacts with both Toll like receptor 4 (TLR4) and Fcgamma receptors (FcgammaRI or FcgammaRIIA) at the cell surface. PMID: 25484053
  34. Data suggest that the specific blocking of immunoglobulin G receptor CD64 by H22(scFv) could be used a possible anti-inflammatory mechanism for potentiating the effect of anti-tumor necrosis factor (TNF) antibodies. PMID: 25517313
  35. high expression of CD64 is an indicator important in the diagnosis of infection and sepsis. PMID: 25180450
  36. An elevated neutrophil CD64 index is associated with both mucosal inflammation and an increased risk for clinical relapse in pediatric Crohn disease. PMID: 24788216
  37. CD64 is expressed on neutrophils and monocytes in periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome during flares PMID: 24623459
  38. FCGR1A (high-affinity IgG Fc receptor 1 (CD64)) was the only marker expressed at significantly higher levels in participants with active tuberculosis. PMID: 24205913
  39. Describe biosensing techniques to evaluate the glycosylation pattern of monoclonal antibodies using the extracellular domains of CD64a. PMID: 24810583
  40. CD64 expression on neutrophils increases significantly in neonates with sepsis and is a useful diagnostic marker for early diagnosis of neonatal infection. PMID: 24463977
  41. As a surveillance biomarker, neutrophil CD64 detected LOS/NEC 1.5 days before clinical presentation, but at the expense of performing 41% additional sepsis evaluations PMID: 24046202
  42. Neutrophil CD64 (nCD64) expression was higher in patients with severe sepsis/septic shock (81.2%) and sepsis(78.8%) as compared to those with traumatic brain injury (5.5%) or controls (0.9%, p < 0.0001). PMID: 23452299
  43. FcgammaRI (CD64) promotes IgG-mediated inflammation, anaphylaxis, and antitumor immunotherapy. PMID: 23293080
  44. Screening identified 19 amino acid mutations contributing to the thermal stability and production rate of rhFcgammaRI PMID: 22967788
  45. Data indicate a tool for measuring antibodies triggering activation of FcgammaRIIIA, FcgammaRIIA or FcgammaRI receptors. PMID: 23023090
  46. Simultaneous quantitative analysis of CD64 and CD35 expression on neutrophils might be useful to distinguish between bacterial and viral infections in rheumatoid arthritis patients. PMID: 22237765
  47. observations suggest that serum amyloid P, at least in part, uses FcgammaRI and FcRgamma to inhibit fibrocyte differentiation PMID: 22493081
  48. Data indicate the simultaneous analysis of CD64 together with CD304 (Neuropilin-1) or the combination of CD11b and CD38 was suitable for the identification of rheumatoid arthritis (RA)patients with high current activity in synovitis. PMID: 22251373
  49. Neutrophil CD64 appears to be a better diagnostic test than traditional hematological assays. PMID: 22420242
  50. Expression of CD64 (FcgammaRI) in skin of patients with acute GVHD. PMID: 21243031

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.
Protein Families
Immunoglobulin superfamily, FCGR1 family
Tissue Specificity
Monocyte/macrophage specific.
Database Links

HGNC: 3613

OMIM: 146760

KEGG: hsa:2209

STRING: 9606.ENSP00000358165

UniGene: Hs.534956

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