Recombinant Mouse 5'-nucleotidase(Nt5e),partial

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Code CSB-AP005101MO
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Product Type CD Antigen
Size $195Purchase it in Cusabio online store
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Uniprot No. Q61503
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Storage Buffer 0.2 μm Filtered 20 mM Tris-HCl, 120 mM NaCl, 4 mM CaCl2, 20% Glycerol, pH 7.5
Alias 5'-nucleotidase;Ecto-5'-nucleotidase;CD73;5'-NT
Species Mus musculus (Mouse)
Biological_Activity Specific activity as determined by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP) is greater than 10000 pmol/min/ug. The orthophosphate product is measured by a malachite green phosphate detection kit.
Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Sequence WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKF
Research Area Cardiovascular
Source Mammalian cell
Gene Names Nt5e
Expression Region 29-550aa
Tag Info C-terminal 6xHis-tagged
Mol. Weight 58.8 kDa
Protein Description Partial
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Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families 5'-nucleotidase family
Database Links

KEGG: mmu:23959

STRING: 10090.ENSMUSP00000034992

UniGene: Mm.244235

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