Mouse 5-Nucleotidase,5-NT ELISA Kit

Code CSB-E04682m
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q61503
Lead Time 3-5 working days
Abbreviation 5-NT
Target Name 5-Nucleotidase,5-NT
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.125 mU/mL-8 mU/mL
Sensitivity 0.031 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 83
Range % 80-85
1:2 Average % 107
Range % 103-110
1:4 Average % 89
Range % 84-93
1:8 Average % 108
Range % 104-112
Recovery
The recovery of mouse 5-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 80-90
EDTA plasma (n=4) 100 95-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
8 2.057 1.968 2.013 1.860
4 1.397 1.402 1.400 1.247
2 0.921 0.900 0.911 0.758
1 0.575 0.601 0.588 0.435
0.5 0.367 0.370 0.369 0.216
0.25 0.255 0.261 0.258 0.105
0.125 0.197 0.190 0.194 0.041
0 0.150 0.156 0.153  
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Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families 5'-nucleotidase family
Database Links

KEGG: mmu:23959

STRING: 10090.ENSMUSP00000034992

UniGene: Mm.244235

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