Mouse 5-Nucleotidase,5-NT ELISA Kit

Code CSB-E04682m
Size 96T,5×96T,10×96T
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Product Details

Target Name
5-Nucleotidase,5-NT
Alternative Names
Nt5e ELISA kit; Nt5 ELISA kit; Nte5'-nucleotidase ELISA kit; 5'-NT ELISA kit; EC 3.1.3.5 ELISA kit; Ecto-5'-nucleotidase ELISA kit; CD antigen CD73 ELISA kit
Abbreviation
5-NT
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.125 mU/mL-8 mU/mL
Sensitivity
0.031 mU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 83
Range % 80-85
1:2 Average % 107
Range % 103-110
1:4 Average % 89
Range % 84-93
1:8 Average % 108
Range % 104-112
Recovery
The recovery of mouse 5-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 80-90
EDTA plasma (n=4) 100 95-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
8 2.057 1.968 2.013 1.860
4 1.397 1.402 1.400 1.247
2 0.921 0.900 0.911 0.758
1 0.575 0.601 0.588 0.435
0.5 0.367 0.370 0.369 0.216
0.25 0.255 0.261 0.258 0.105
0.125 0.197 0.190 0.194 0.041
0 0.150 0.156 0.153  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse 5-NT ELISA Kit was designed for the quantitative measurement of Mouse 5-NT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.125 mU/mL-8 mU/mL and the sensitivity is 0.031 mU/mL.

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Target Background

Function
(From Uniprot)
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Gene References into Functions
  1. Deletion of CD73 in mice leads to aortic valve dysfunction similar to that induced by high-fat diet suggesting important role of this surface protein in maintaining heart valve integrity. PMID: 28192180
  2. We establish that the mouse Nt5e minimal promoter transcriptional activity is negatively regulated by an Elf2-like Ets-related transcription factor in activated mouse liver myofibroblasts PMID: 28667437
  3. CD73 derived adenosine production slows down migration of Langerin+ dendritic cells from skin to lymph nodes. This may be a crucial mechanism to avoid over boarding immune reactions against haptens. PMID: 28743609
  4. Compared to adults, young WT mice failed to control S. pneumoniae infection after vaccination and this was associated with lower levels of CD73 on innate B cells. We hypothesized that pharmacological activation of A2a receptor may improve Pneumovax 23 immunization in young WT mice PMID: 29377929
  5. The 5'-ectonucleotidase CD73 has a key role in regulating macrophage immune responses during efferocytosis by converting AMP released from apoptotic cells into adenosine to suppress multiple pro-inflammatory cytokines. PMID: 28060378
  6. findings demonstrate the critical role of CD73 in the regulation of infection by the enteric pathogen Salmonella and implicate adenosine as an important host-derived factor that mediates host-microbe interaction PMID: 28717030
  7. Data show that AMP deaminase 3 (Ampd3)-/-/5'-nucleotidase (CD73)-/- knockout mice are more sensitive to AMP-induced hypometabolism than mice with a single enzyme deficiency, which are more sensitive than wild type. PMID: 28746349
  8. This study evaluated the role of CD73 in the accumulation and adhesion of pulmonary macrophages in response to thoracic irradiation. PMID: 28325757
  9. Deletion of CD39 and CD73 or both caused an inhibition of the microglia ramified phenotype in the brain with a reduction in the length of processes, branching frequency and number of intersections with Sholl spheres. In vitro, unlike wild-type microglia, cd39-/- and cd73-/- microglial cells were less complex and did not respond to ATP with the transformation into a more ramified phenotype. PMID: 28376099
  10. CD73 expression is regulated during experimental autoimmune encephalomyelitis, this enzyme is not absolutely required to either promote or limit Th17 cell expansion or experimental autoimmune encephalomyelitis severity. PMID: 28288184
  11. High NT5E expression is associated with Radiation-Induced Lung Fibrosis. PMID: 26921334
  12. Ablation of CD73 minimally effects in vivo thrombosis, but increased CD39 expression on hematopoietic-derived cells, especially monocytes, attenuates in vivo arterial thrombosis. PMID: 27417582
  13. CD73 on T cells plays an important anti-inflammatory role in transverse aortic constriction-induced heart failure PMID: 28404626
  14. In this study, we investigated whether blockade of the A2A adenosine receptor with a selective antagonist and a CD73 inhibitor may increase the efficacy of a dendritic cell-based cancer vaccine PMID: 28349824
  15. Data indicate that adenosine and CGS21680 upregulate CD39 and CD73 via E2F-1 and CREB. PMID: 27430240
  16. CD73-siRNA-loaded ChLa NPs may be considered as a promising therapeutic tool for cancer therapy; however, further in vivo investigations are necessary PMID: 26733167
  17. CD73/adenosine pathway involves the immunomodulatory function of mesenchymal stem cells in autoimmune responses. PMID: 26650818
  18. CD73 facilitates AS by promoting migration, proliferation, and foam cell transformation of vascular smooth muscle cells and elevating serum lipid levels. PMID: 26506509
  19. Low CD73 expression plays a role in melanoma progression. PMID: 26545615
  20. These data reveal a novel function of CD73 ectonucleotidase in arresting CD8(+) T-cell differentiation and support the idea that CD73-driven adenosine production by Tc17 cells may promote stem cell-like properties in Tc17 cells. PMID: 26331349
  21. gammadelta T cells expressed different CD73 amounts in different stages of experimental autoimmune uveitis. Low CD73 on gammadelta T cells enhanced Th17 response-promoting activity. Failure to express CD73 decreased enhancing and suppressive effects of gammadelta T cells. PMID: 26919582
  22. Activation of A3, A2A and A1 Adenosine Receptors in CD73-Knockout Mice Affects B16F10 Melanoma Growth, Neovascularization, Angiogenesis and Macrophage Infiltration PMID: 26964090
  23. CD73 activity from any host cell type is not required for the monocyte/macrophage polarization in the peritoneum towards a pro- or an anti-inflammatory phenotype in vivo PMID: 26258883
  24. CD73 is not necessary for development of acute lung injury following influenza A virus infection. PMID: 26432867
  25. The ecto-5'-nucleotidase derived extracellular formation of adenosine does not contribute substantially to adenosine's well known cardioprotective effect in early phase ischemic preconditioning. PMID: 26261991
  26. CD73 expression was downregulated during acute infection with T. gondii, leading to impaired capacity to produce adenosine. PMID: 25389034
  27. Expression of NPP1 and 5'-nucleotidase by valve interstitial cells promotes the mineralization of the aortic valve through A2aR and a cAMP/PKA/CREB pathway. PMID: 25644539
  28. The CD73 deficiency significantly increased the levels of proinflammatory cytokines in mice. PMID: 25805696
  29. our results support the view that CD73 fine-tunes antimycobacterial immune responses. PMID: 26150535
  30. CD73 expression attenuates inflammation during murine Salmonellosis and impairs immunity, leading to increased bacterial colonization and prolonged infection. PMID: 24670982
  31. CD73 is dispensable for normal CD8+ T-cell differentiation and function PMID: 25490556
  32. The mouse model deficient in CD73, with other targeted mutant mice with vascular mineralization, attests to the presence of a complex pro-mineralization/anti-mineralization network that prevents ectopic tissue mineralization. PMID: 25486201
  33. In the urothelium of animals with bladder cancer there was an increase in the expression of ecto-5'-nucleotidase. PMID: 24464643
  34. Data show that CD73 (5' nucleotidase) deficiency resulted in greater disease incidence. PMID: 25681339
  35. the absence of CD73-generated adenosine led to the increased susceptibility to Toxoplasma gondii infection in CD73 knockout mice. PMID: 25452548
  36. CD73 enhances endothelial barrier function and sprouting in blood but not lymphatic vasculature. PMID: 25402681
  37. Tetraspanin CD9 and ectonucleotidase CD73 identify an osteochondroprogenitor population with elevated osteogenic properties. PMID: 25564652
  38. CD73 and TNAP play interactive roles to metabolize luminally applied 5'-AMP in the renal vasculature such that inhibition of both is required to inhibit the production of adenosine. PMID: 24990899
  39. CD73-dependent adenosine signaling is prominent in the mature GC and required for establishment of the long-lived PC compartment, thus identifying a novel role for CD73 in humoral immunity PMID: 24664100
  40. that extracellular adenosine, generated in tandem by ecto-enzymes CD39 and CD73, promotes dermal fibrogenesis. PMID: 24266925
  41. CD73-dependent production of extracellular adenosine and endothelial Adora2b signaling protects kidney during diabetic nephropathy. PMID: 24262796
  42. Isoflurane causes TGF-beta1-dependent induction of renal tubular CD73 and adenosine generation to protect against renal ischemia and reperfusion injury. PMID: 23423261
  43. identify CD73 as a TCR ligand-induced cell surface protein that distinguishes gammadeltaTCR-expressing CD4(-)CD8(-) progenitors that have committed to the gammadelta fate from those that have not yet done so PMID: 24493796
  44. CD73 mRNA levels were also dramatically decreased in human liver biopsies from hepatitis C and nonalcoholic fatty liver disease patients PMID: 23729294
  45. Data indicate that CD73 promoted metastasis through the activation of both A2A and A2B receptors. PMID: 23964122
  46. Our data provide evidence for a role of CD73 in the regulation of learning and memory and psychomotor coordination PMID: 23274765
  47. findings demonstrate that CD73-expressing exosomes produced by Treg cells following activation contribute to their suppressive activity through the production of adenosine PMID: 23749427
  48. Our novel findings suggest an important immune-regulatory role of CD73 in regulation of diabetes development PMID: 23956420
  49. NT5E (and prostatic acid phosphatase) are the main AMP ectonucleotidases in somatosensory neurons. PMID: 23825434
  50. CD73 is upregulated in immune cells after ischemia/reperfusion in the myocardium. PMID: 23720442

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
5'-nucleotidase family
Database Links
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