Mouse 5-Nucleotidase,5-NT ELISA Kit

Instructions
Code CSB-E04682m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name 5-Nucleotidase,5-NT
Abbreviation 5-NT
Uniprot No. Q61503
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.125 mU/mL-8 mU/mL
Sensitivity 0.031 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 83
Range % 80-85
1:2 Average % 107
Range % 103-110
1:4 Average % 89
Range % 84-93
1:8 Average % 108
Range % 104-112
Recovery
The recovery of mouse 5-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 80-90
EDTA plasma (n=4) 100 95-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/ml OD1 OD2 Average Corrected
8 2.057 1.968 2.013 1.860
4 1.397 1.402 1.400 1.247
2 0.921 0.900 0.911 0.758
1 0.575 0.601 0.588 0.435
0.5 0.367 0.370 0.369 0.216
0.25 0.255 0.261 0.258 0.105
0.125 0.197 0.190 0.194 0.041
0 0.150 0.156 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families 5'-nucleotidase family
Database Links

KEGG: mmu:23959

STRING: 10090.ENSMUSP00000034992

UniGene: Mm.244235

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