The DNA to Amino Acid Translation Tool is a simple yet powerful online resource that allows researchers to convert nucleotide sequences into their corresponding amino acid sequences.
It is designed to support gene annotation, protein prediction, cloning validation, and molecular biology education.
Whether you're verifying a cloned gene or exploring open reading frames (ORFs), this tool helps visualize and interpret translation outcomes with codon-level clarity.
Genetic Code Table: Standard Code (1)
Reading Frame: +1 (start at position 1)
Start Codon: AUG (M)
Stop Codons: UAA, UAG, UGA
The DNA to Amino Acid Translation Tool first transcribes your DNA sequence into messenger RNA (mRNA) and then translates it into a protein sequence by reading every three nucleotides (codon) according to your selected genetic code table.
Key features include:
| 1st Base | 2nd Base | 3rd Base | Amino Acid |
|---|---|---|---|
| U | U | U, C | Phe (F) |
| U | U | A, G | Leu (L) |
| A | U | G | Met (M) (Start) |
| U | A | A, G | Stop |
The complete genetic code table contains 64 combinations. This tool supports multiple variant code tables, such as mitochondrial codes.
- The amino acid sequence
- Amino acid composition analysis
- Mapped codons (if selected)
Want a quick walkthrough? Watch the short video below to see how to use this tool in action.
The DNA to Amino Acid Translation Tool is designed to support a wide range of research and educational applications in molecular biology and bioinformatics.
✔️ Gene Cloning and Expression Verification
Confirm that cloned DNA sequences translate into the expected protein products before expression vector construction or synthesis.
✔️ Protein Design and In Silico Translation
Predict open reading frames (ORFs) and identify coding sequences for protein design, mutagenesis, or codon optimization.
✔️ Teaching and Learning
Demonstrate the central dogma in molecular biology—DNA → mRNA → protein—through hands-on visualization of codon-to-amino-acid translation.
✔️ Genome Annotation and Bioinformatics Analysis
Rapidly screen DNA sequences from genomic data to identify putative coding regions and annotate candidate genes.
✔️ Synthetic Biology and Construct Validation
Validate designed synthetic gene sequences by checking their translated amino acid output before synthesis and downstream cloning.
Q1: What is a reading frame?
A reading frame determines how the nucleotide sequence is divided into codons. Each frame (starting at base 1, 2, or 3) results in a different set of codons and therefore a different amino acid sequence.
Q2: What if my input sequence contains non-ATCG characters?
The tool automatically filters out invalid characters, but for best results, please input clean DNA sequences.
Q3: Can I analyze sequences from mitochondrial genes?
Yes. You can select specific mitochondrial genetic code tables that reflect the codon usage in mitochondria from various organisms.
Q4: What does the amino acid composition analysis show?
It displays the count and percentage of each amino acid in the translated sequence, helping you understand sequence bias or abundance.
Q5: What happens if a stop codon is encountered?
The translation process will stop at the first stop codon unless you modify the sequence or settings to allow full translation.
Your feedback helps us improve!
If you encounter any issues, have suggestions, or would like to request new features, please email us at [email protected] with the subject line "DNA Translation Tool Feedback".
We appreciate your input and aim to enhance this tool for the scientific community.
