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Mammalian Cell Display Service

Mammalian Cell Display Service

Mammalian cell display platform, differing from such other types of display techniques as phage or yeast display, is an advanced platform designed for optimizing therapeutic antibodies' developability. Mammalian cells cultured in dishes share many characteristics with humans in clinical trials and industrialized cells for antibody production. This technique serves as a cutting-edge tool for antibody engineering, such as affinity maturation and humanization.

Mammalian cell display is very well suitable to engineering antibody. Affinity maturation is a major application.

Swapping CDRs across species to meet different therapeutic demands, such as humanization, caninization, and others.

This advanced technique is compatible well with a full spectrum of Ab formats, from IgG, to nanobody, to TCR, etc.

CUSABIO Presents:

Human-like PTM

Fine-tuned biophysical property

Better developability

Best for antibody engineering

Mammalian Cell Protein Quality Control System

	Protein quality control
	Golgi	ER	Membrane
Phage	✖	✖	✖
Yeast	✔	✔	✖
Mammalian	✔	✔	✔

Mammalian Cell VS. Phage Display

Application 1: Affinity Maturation

● The affinity of customer's mAb was enhanced 100-fold by mutating one AA in CDR3.

Target	Analyte	K_on (1/Ms)	K_off (1/s)	KD (M)	RU²
Confidential_1	Ab54 (WT)	3.76E+05	1.24E-02	3.30E-08	0.26
Confidential_1	Ab54-17	3.83E+05	1.06E-04	2.77E-10	0.97

● The affinity of customer's mAb against cynomolgus antigen was enhanced 100-fold by mutating one AA in each CDR3, while retaining the affinity to human antigen.

Target	Analyte	K_on (1/Ms)	K_off (1/s)	KD (M)	RU²
Confidential_2 (Human)	Ab16 (WT)	4.13E+05	1.85E-04	4.48E-10	3.44
Confidential_2 (Human)	Ab16-03	4.53E+05	2.09E-04	4.61E-10	1.79
Confidential_2 (Cynomolgus)	Ab16 (WT)	1.81E+05	5.24E-03	2.90E-08	1.87
Confidential_2 (Cynomolgus)	Ab16-03	8.92E+05	4.47E-04	5.01E-10	4.32

● Customers' in vivo data further proves good quality of the engineered variants.

More affinity maturation

TCR affinity maturation

Blocking activity of small molecules (chemical compound)

Application 2: Humanization

● Customer's mAb was humanized through a library specifically designed for screening high-affinity molecules, and the affinity of humanized antibodies could be 10-fold higher than the parent molecule.

Target	Analyte	K_on (1/Ms)	K_off (1/s)	KD (M)	RU²
Confidential_3	Ab01 (WT)	4.06E+04	3.11E-05	7.66E-10	0.45
	Ab01-01	2.17E+04	7.06E-06	3.25E-10	1.11
	Ab01-02	7.22E+04	7.49E-06	1.04E-10	0.14
	Ab01-03	5.61E+04	4.78E-05	8.52E-10	2.76
	Ab01-04	2.79E+04	2.38E-06	8.53E-11	2.59
	Ab01-05	4.89E+04	4.25E-05	8.69E-10	3.73

● WT and the engineered molecules were also compared by ELISA and flow cytometry.

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