Surface Plasmon Resonance (SPR) is a biosensing technology based on optical phenomena. By modifying biomolecules (such as antibodies, receptors, nucleic acids, etc.) on the surface of a sensor chip, the binding of target molecules to the surface molecules causes a change in the surface plasmon resonance frequency. This change is positively correlated with the amount of molecular binding, enabling real-time, dynamic, and label-free detection of interaction strength (Kd value) and binding kinetics (Kon/Koff).
CUSABIO offers comprehensive SPR detection services using both Biacore and WeSPR platforms, covering a wide range of samples from small molecule drug candidates to high molecular weight protein antibodies (including peptides, DNA, RNA, polysaccharides, cells, and viruses). Our services support antibody screening, drug development, and basic research.
Surface Plasmon Resonance (SPR) Principle Diagram
Service Advantages:
Advanced Technology Platforms Multiple devices (Biacore 8K/T200, WeSPRTM) cater to diverse needs, supporting basic research to industrial-scale high-throughput detection.
Specialized Support Exclusive SPR detection services for membrane proteins, helping clients understand complex biomolecular interactions.
Experienced Technical Team Quick response with customized experimental solutions. Dedicated project managers provide real-time updates.
Application Scenarios:
Target Validation
Measure binding kinetics between drug targets and small/large molecule drugs.
Antibody Optimization
Affinity maturation and cross-reactivity assessment.
ADC Drug Development
Analyze antibody-toxin conjugate stability and receptor-ligand binding patterns.
Molecular Interaction Mechanisms
Quantify receptor-ligand and nucleic acid-protein interactions.
Biomarker Validation
Trace biomarker detection and antibody specificity verification.
Assay Kit Optimization
Reaction condition optimization and batch consistency validation.
Enzyme Kinetics Studies
Determine enzyme-substrate binding constants and allosteric enzyme mechanisms.
Virus-Host Interaction Studies
Viral entry mechanism analysis, antiviral drug screening, and vaccine development support.
Service Process:
Service Cycle: 3-4 weeks
1
Submit Sample Information Form
Specify analyte type (protein/small molecule/antibody, etc.) and detection requirements.
2
Professional Pre-Evaluation
Small molecule analytes: Directly proceed to SPR experiments.
Protein interactions: Prioritize ELISA functional activity screening.
3
Place Order and Send Samples
4
Experiment Execution
5
Report Delivery
Provide raw data, fitting curves, and kinetic parameter tables.
Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody (CSB-RA004843MA01HU) with an affinity constant of 3.16 nM as detected by SPR Assay (Biacore T200).
Human CLDN6 Monoclonal Antibody (CSB-RA005508MA1HU) captured on Protein A Chip can bind Human CLDN6 Full Length VLP Protein with an affinity constant of 6.58 nM as detected by MetaSPR Assay (WeSPRTM 200).
● Detection of Small Molecule-Protein Binding Affinity
Human SSTR2 Full Length Protein captured on LifeDiscTM COOH chip (in presence of FOS-12) can bind [Tyr3]Octreotate with an affinity constant of 22.9 μM as detected by MetaSPR Assay (WeSPRTM One).
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