Bovine Insulin-like growth factor II(IGF2) ELISA kit

Code CSB-EL011088BO
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Bovine IGF2 ELISA Kit was designed for the quantitative measurement of Bovine IGF2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.81 ng/mL.

Target Name insulin-like growth factor 2 (somatomedin A)
Alternative Names IGF2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
Abbreviation IGF2
Uniprot No. P07456
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 100-114
1:2 Average % 103
Range % 101-106
1:4 Average % 87
Range % 84-91
1:8 Average % 94
Range % 90-96
Recovery
The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 88 85-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2000 1.958 1.952 1.955 1.858
1000 1.166 1.076 1.121 1.024
500 0.671 0.654 0.663 0.566
250 0.398 0.389 0.394 0.297
125 0.263 0.254 0.259 0.162
62.5 0.177 0.169 0.173 0.076
31.25 0.135 0.129 0.132 0.035
0 0.096 0.097 0.097  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
Gene References into Functions
  1. Methylation pattern in a CpG island of the IGF2 gene in cumulus cells from 1-3 mm and >/= 8.0 mm follicles and the effects of in vitro maturation on this pattern. PMID: 24174298
  2. Our results provide evidence that polymorphisms in the IGF2 gene are associated with growth traits, and may be used for marker-assisted selection in beef cattle breeding program PMID: 24374893
  3. The identification of the polymorphisms in the IGF2 gene that were significantly associated with growth traits in cattle. PMID: 23957672
  4. Polymorphisms in the IGF2 gene are associated with cattle growth traits. PMID: 23900197
  5. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
  6. Sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methylation patterns among bulls was observed. PMID: 22128039
  7. The reduction in IGFs mRNA level after 5 days of life in the duodenum (IGF-1 and IGF-2) and in the jejunum (IGF-1) was associated with reduction in villi length (duodenum and jejunum) and the increase of crypt depth (duodenum). PMID: 22439332
  8. Changes in the methylation pattern of IGF2 during in vitro maturation were different between incompetent and competent oocytes, and this characteristic may be useful as a molecular marker in studies of oocyte competence in cattle. PMID: 20833870
  9. Data describes the long-term changes to skeletal muscle growth and IGF1, 2, 1R, and 2R mRNA expression in progeny after exposure to high and low levels of maternal nutrient intake during the first two trimesters of gestation in the bovine. PMID: 21056085
  10. findings support work which suggests that the insulin-like growth factor 2 locus is an important biological regulator of milk production in dairy cattle PMID: 20822563
  11. effects of insulin, IGF-I and IGF-II on apoptosis and cell proliferation in bovine blastocysts in vitro PMID: 12112582
  12. IGF2 is subjected to extensive transcriptional regulation through multiple promoters, alternative splicing and polyadenylation, as well as genetic imprinting.IGF2 regulation is age-, tissue-, promoter-, and allele-specific PMID: 16120826
  13. Identification of a previously unknown differentially methylated region in exon 10 of the bovine IGF2 gene. PMID: 16644179
  14. Indel polymorphism associated with breeding value in bulls. PMID: 17242995
  15. The role of insulin-like growth factor 2 (IGF2) and the regulation of the IGF2 receptor (IGF2R) during follicular development were studied. PMID: 17360960
  16. Embryonic tissues from NT-derived embryos had higher expression of IGF-II mRNA than in vitro production embryonic tissues. PMID: 17628655
  17. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
  18. study to assess the mRNA expression of IGF-I, IGF-II, IGF-IR and IGF-IIR in bovine oocytes and different stages of preimplantation embryos PMID: 19013732
  19. Dats detected that SNPs of IGF2 gene has close relationship with carcass and meat quality traits in Qinchuan cattle PMID: 19073573
  20. Hypomethylation trends in the intergenic region of the imprinted IGF2 and H19 genes in cloned cattle. PMID: 19282114
  21. Among embryos developing in vivo, the level of DNA methylation in the IGF2 intragenic DMR was significantly lower in female than in male blastocysts PMID: 19341790

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Subcellular Location Secreted.
Protein Families Insulin family
Database Links

KEGG: bta:281240

STRING: 9913.ENSBTAP00000017372

UniGene: Bt.106739

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