Bovine Insulin-like growth factor II(IGF2) ELISA kit

Instructions
Code CSB-EL011088BO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name insulin-like growth factor 2 (somatomedin A)
Alternative Names C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018|OTTHUMP00000011157|insulin-like growth factor 2|insulin-like growth factor II|insulin-like growth factor type 2|putative insulin-like
Abbreviation IGF2
Uniprot No. P07456
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 100-114
1:2 Average % 103
Range % 101-106
1:4 Average % 87
Range % 84-91
1:8 Average % 94
Range % 90-96
Recovery
The recovery of bovine IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 90-100
EDTA plasma (n=4) 88 85-91
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
2000 1.958 1.952 1.955 1.858
1000 1.166 1.076 1.121 1.024
500 0.671 0.654 0.663 0.566
250 0.398 0.389 0.394 0.297
125 0.263 0.254 0.259 0.162
62.5 0.177 0.169 0.173 0.076
31.25 0.135 0.129 0.132 0.035
0 0.096 0.097 0.097  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days
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Target Data

Function The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: bta:281240

STRING: 9913.ENSBTAP00000017372

UniGene: Bt.106739

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