Human Alanine Aminotransferase,ALT/GPT ELISA Kit

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Target Background

Name: Human Alanine Aminotransferase,ALT/GPT ELISA Kit
Brand: CUSABIO
SKU: CSB-E10082h

Product Details

Target Name

glutamic-pyruvate transaminase (alanine aminotransferase)

Alternative Names

AAT1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; Alanine aminotransferase ELISA Kit; ALAT1_HUMAN ELISA Kit; ALT1 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; Glutamic alanine transaminase 1 ELISA Kit; Glutamic pyruvate transaminase (alanine aminotransferase) ELISA Kit; Glutamic pyruvic transaminase 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit; GPT 1 ELISA Kit; gpt ELISA Kit; GPT1 ELISA Kit

Abbreviation

GPT

Uniprot No.

P24298

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 uIU/mL-1000 uIU/mL

Sensitivity

3.9 uIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ALT/GPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	84
	Range %	80-88
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	87-96

Recovery

The recovery of human ALT/GPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	96	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

uIU/ml	OD1	OD2	Average	Corrected
1000	2.542	2.687	2.615	2.516
500	1.706	1.717	1.712	1.613
250	1.003	1.089	1.046	0.947
125	0.626	0.597	0.612	0.513
62.5	0.317	0.304	0.311	0.212
31.2	0.255	0.239	0.247	0.148
15.6	0.185	0.171	0.178	0.079
0	0.103	0.095	0.099

Materials provided

A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human ALT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled ALT antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human GPT ELISA Kit was designed for the quantitative measurement of Human GPT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 uIU/mL-1000 uIU/mL and the sensitivity is 3.9 uIU/mL.

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Customer Reviews and Q&A

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■ Q&A

I want to find out more about your human ALT/GPT ELISA Kit
I want to measure the human ALT from the serum of liver humanized mice
Hence, I need the kit to be human specific and not to pick up the signal from mouse ALT
Would CSB-E10082h serve the purpose?

Thanks for your inquiry.
Reviewing from your research purpose, human ALT is the reasonable consideration.Our elisa kits #CSB-E10082h is for determination for content,not for detectiono of enzyme activity.
Strictly speaking,if the concentrate of ALT locates in the detection range of kit #CSB-E10082h ,it can be detected out.Meanwhile,a point need to pay attention,as your sample is from serum of mice,then there would be a matrix problem.
And reviewing from your description,there will be supersession when injecting the natural ALT (human liver ALT) or transcription by other ways into mice.
Based on comprehensive analysis,you can consider to do pre-testing.Pls let me know if you have any further questions. Thank you.
Additionally,you need to do further validation on how to evaluate whether the modeling of humanized mice is successful or not.Pls let me know if you have any further questions. Thank you.

May I know the cross reactivity about CSB-E10082h. Can you help us to check and revert?Does the human ALT ELISA Kit have any cross reaction with mouse ALT?

Thanks for your inquiry.
#CSB-E10082h,Human Alanine Aminotransferase,ALT/GPT ELISA Kit
We checked and the above kit has no cross reactions with either mouse ALT or rat ALT.Pls let me know if you have any further questions. Thank you.

If the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

Thanks for your inquiry. The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation. Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Gene References into Functions

a relationship between NAFLD with high ALT and lower BMD in the general U.S. population PMID: 29897928
The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
In men with hepatic steatosis, we found that elevated serum ALT levels were associated with lower serum SHBG levels. PMID: 28540984
Our results using Mendelian randomization suggest that ALT reduces the risk of ischaemic heart disease (IHD), probably through reducing triglyceride levels. PMID: 28007909
Measurements of serum alpha-fetoprotein (AFP) levels could be helpful in detecting progression or recurrence in treated patients with hepatitis B virus (HBV) - hepatocellular carcinoma (HCC) and normal alanine aminotransferase (ALT). PMID: 27304617
Baseline alanine transaminase (ALT) and a hepatitis B surface antigen (HBsAg) above 1000 IU/ml were independent predictive factors for ALT elevation. PMID: 27935953
High serum alanine aminotransferase expression is associated with metabolic syndrome. PMID: 27390880
The data of this study confirmed the genetic heterogeneity of childhood-onset pure HSP, with SPG4/SPAST and SPG3A/ATL1 being the most frequent forms. PMID: 27260292
Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
Liver fat content and serum ALT were inversely correlated with bone mineral density in middle-aged and elderly men. PMID: 26791408
Age, female gender, and elevated ALT can help identifying cases with biliary etiology of acute pancreatitis. PMID: 26664483
IBS [irritable bowel syndrome ] may be an important condition in certain patients with elevated ALT levels and MS [ metabolic syndrome ]. PMID: 26632395
Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GPT1 rises due to up-regulation of hepatic expression of GPT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
Elevated aminotransferase levels, even within the normal range, were significantly associated with increased risk of all external-cause mortalities, including suicide, and injury. PMID: 26446640
There are significant relationships of elevated ALT levels with cardiometabolic risk factors (obesity, hypertension, hypercholesteremia, hyperuricemia, hypertriglyceridemia) and several sex-related differences in rural Chinese. PMID: 26160405
ALT was significantly associated with elevated levels of triacylglycerols, hyperglycemia, and obesity. In patients with metabolic syndrome, one must consider liver involvement if the patient has ALT levels in the upper third of the reference range. PMID: 25874513
Peginterferon alfa-2a is associated with elevations in alanine aminotransferase at the end of treatment in chronic hepatitis C patients with sustained virologic response. PMID: 24937007
A prospective association between ALT levels and metabolic syndrome highlights the value of ALT levels, even mild ALT elevations within the normal range, as a predictor of the MetS risk in male schizophrenia patients. PMID: 24819732
Serum glutamate pyruvate transaminase levels were elevated postoperatively in recipients of an orthotopic liver transplant from a brain-dead donor receiving extracorporeal membrane oxygenation. PMID: 25543901
Statistically significant differences were observed in the mean values of GPT in type 1 diabetics compared to type 2 and control groups PMID: 24849491
Alanine aminotransferase showed associations with BMI, glycaemia, lipids, and creatinine. The relationship between alanine aminotransferase and age was best expressed by polynomial regression. PMID: 24953204
Serum alanine aminotransferase has limited predictive value for liver disease in chronic hepatitis C PMID: 25219834
Role of insulin resistance and adipocytokines on serum alanine aminotransferase in obese patients with type 2 diabetes mellitus PMID: 23884827
In nonalcoholic fatty liver disease, adipose tissue insulin resistance (but not hepatic insulin resistance) and liver triglyceride content are major factors in the elevation of plasma aminotransferase levels. PMID: 25145475
elevated ALT is common in the Korean population; it is associated with metabolic disorders (obesity, insulin resistance, or metabolic syndrome) in the majority of patients PMID: 24275711
ALT and visceral adipose tissue affect metabolic risk factors in a middle-aged Japanese population PMID: 24500143
PNPLA3 I148M polymorphism is associated with elevated alanine transaminase levels in Mexican indigenous and Mestizo populations. PMID: 24691744
Among patients with chronic HCV infection, daily consumption of filtered coffee may have a beneficial effect on the stabilization of ALT levels. PMID: 24349501
A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
Elevated ALT is associated with developing depressive symptoms, suggesting that non-alcoholic fatty liver disease may represent an independent modifiable risk factor for depression. PMID: 23522007
fibrosis is not rare in Chinese CHB patients with persistent normal alanine aminotransferase, especially HBeAg-positive patients over 30 years old PMID: 24205292
Elevated serum ALT levels are prevalent among elderly agricultural and fishing population workers in Taiwan. PMID: 24158044
Elevated serum alanine aminotransferase level was independently associated with an increased risk of intracerebral hemorrhage in East Asian men. PMID: 23880909
Normal to near-normal serum aminotransferase levels are associated with metabolic disorders. PMID: 23794308
Data from cross-sectional study in children/adolescents in Republic of Korea suggest normal ALT/GPT serum reference values; ALT/GPT serum level appears effective as biomarker in predicting cardiovascular/metabolic syndrome risk in population studied. PMID: 23403445
Among HIV/HCV-coinfected persons, risky drinking was associated with a higher adjusted mean AST (62.2 vs. 51.4 U/L; adjusted ratio of means 1.2, 95% CI [1.07, 1.37], p = .003) and ALT (51.3 vs. 41.6 U/L; compared with non-risky drinking PMID: 23384374
Nonalcoholic fatty liver disease and metabolic syndrome were associated with ALT levels in a male population from southern China. PMID: 22975640
ALT[Alanine transaminase] may be a marker of an underlying etiology relating to the paradoxical associations of diabetes and IHD at a population level. PMID: 22954960
An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold. PMID: 22389715
In a cross-sectional analysis of Taiwanese industrial employees, elevated ALT associated with MS, but in subjects who did not meet MS criteria, elevated ALT by itself did not associate with IFG/T2DM. PMID: 21715038
Patients with HLA-DQA1 and DRB1 genotypes have higher alanine aminotransferase elevation, whereas HLA-negative cases have a higher incidence of Gilbert's syndrome. PMID: 22357454
In a cross-sectional study, Chinese adolescents and young adults with more metabolic syndrome components were more likely to suffer from elevated ALT PMID: 22177542
alanine aminotransferase levels largely within the normal range, there was an inverse association between ALT and all-cause mortality and cardiovascular events PMID: 22158663
the COMT polymorphism affects serum ALT activity to maintain liver function PMID: 22293393
The magnitude of the association of PNPLA3 with liver enzymes is driven by the size of abdominal fat. PMID: 22140488
discriminates persons infected with hepatitis C virus from those at low risk of liver disease, but would be considered elevated in a large proportion of the U.S. population PMID: 21987480
An hepatitis B virus DNA level of >/=5 log10 copies/mL and an alanine aminotransferase (ALT) level of >20 to PMID: 21692932
The prevalence of metabolic syndrome (MS) and risk factors for and association with elevated alanine aminotransferase (ALT) as markers of hepatic injury in a large Hispanic health disparity cohort with high rates of obesity, was studied. PMID: 21720553
Subjects with metabolic syndrome had significantly higher plasma levels of alanine aminotransferase. PMID: 21040936
Nrmal levels of ALT are associated with metabolic and androgenic phenotypes, i women of reproductive age. PMID: 20961180

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Subcellular Location

Cytoplasm.

Protein Families

Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily

Tissue Specificity

Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

Database Links

HGNC: 4552

OMIM: 138200

KEGG: hsa:2875

STRING: 9606.ENSP00000378408

UniGene: Hs.103502

Code	CSB-E10082h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.