Human Alanine Aminotransferase,ALT/GPT ELISA Kit

Instructions
Code CSB-E10082h
Size 96T,5×96T,10×96T

Product Details

Target Name glutamic-pyruvate transaminase (alanine aminotransferase)
Alternative Names AAT1, ALT1, GPT1, glutamic pyruvate transaminase|glutamic-alanine transaminase 1
Abbreviation GPT
Uniprot No. P24298
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 uIU/mL-1000 uIU/mL
Sensitivity 3.9 uIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ALT/GPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 92-104  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of human ALT/GPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-94  
EDTA plasma (n=4) 96 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
uIU/ml OD1 OD2 Average Corrected  
1000 2.542 2.687 2.615 2.516  
500 1.706 1.717 1.712 1.613  
250 1.003 1.089 1.046 0.947  
125 0.626 0.597 0.612 0.513  
62.5 0.317 0.304 0.311 0.212  
31.2 0.255 0.239 0.247 0.148  
15.6 0.185 0.171 0.178 0.079  
0 0.103 0.095 0.099    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

I want to find out more about your human ALT/GPT ELISA Kit
I want to measure the human ALT from the serum of liver humanized mice
Hence, I need the kit to be human specific and not to pick up the signal from mouse ALT
Would CSB-E10082h serve the purpose?

A:
Thanks for your inquiry.
Reviewing from your research purpose, human ALT is the reasonable consideration.Our elisa kits #CSB-E10082h is for determination for content,not for detectiono of enzyme activity.
Strictly speaking,if the concentrate of ALT locates in the detection range of kit #CSB-E10082h ,it can be detected out.Meanwhile,a point need to pay attention,as your sample is from serum of mice,then there would be a matrix problem.
And reviewing from your description,there will be supersession when injecting the natural ALT (human liver ALT) or transcription by other ways into mice.
Based on comprehensive analysis,you can consider to do pre-testing.Pls let me know if you have any further questions. Thank you.
Additionally,you need to do further validation on how to evaluate whether the modeling of humanized mice is successful or not.Pls let me know if you have any further questions. Thank you.
Q:

May I know the cross reactivity about CSB-E10082h. Can you help us to check and revert?Does the human ALT ELISA Kit have any cross reaction with mouse ALT?

A:
Thanks for your inquiry.
#CSB-E10082h,Human Alanine Aminotransferase,ALT/GPT ELISA Kit
We checked and the above kit has no cross reactions with either mouse ALT or rat ALT.Pls let me know if you have any further questions. Thank you.
Q:

If the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

A:
Thanks for your inquiry. The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation. Pls let me know if you have any further questions. Thank you.
 Customer Reviews

Target Data

Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Subcellular Location Cytoplasm
Protein Families Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Database Links

HGNC: 4552

OMIM: 138200

KEGG: hsa:2875

STRING: 9606.ENSP00000378408

UniGene: Hs.103502

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