Human Alanine Aminotransferase,ALT/GPT ELISA Kit

Instructions
Code CSB-E10082h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name glutamic-pyruvate transaminase (alanine aminotransferase)
Alternative Names AAT1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; Alanine aminotransferase ELISA Kit; ALAT1_HUMAN ELISA Kit; ALT1 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; Glutamic alanine transaminase 1 ELISA Kit; Glutamic pyruvate transaminase (alanine aminotransferase) ELISA Kit; Glutamic pyruvic transaminase 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit; GPT 1 ELISA Kit; gpt ELISA Kit; GPT1 ELISA Kit
Abbreviation GPT
Uniprot No. P24298
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 uIU/mL-1000 uIU/mL
Sensitivity 3.9 uIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ALT/GPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 92-104  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 92  
Range % 87-96  
Recovery
The recovery of human ALT/GPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-94  
EDTA plasma (n=4) 96 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
uIU/ml OD1 OD2 Average Corrected  
1000 2.542 2.687 2.615 2.516  
500 1.706 1.717 1.712 1.613  
250 1.003 1.089 1.046 0.947  
125 0.626 0.597 0.612 0.513  
62.5 0.317 0.304 0.311 0.212  
31.2 0.255 0.239 0.247 0.148  
15.6 0.185 0.171 0.178 0.079  
0 0.103 0.095 0.099    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I want to find out more about your human ALT/GPT ELISA Kit
I want to measure the human ALT from the serum of liver humanized mice
Hence, I need the kit to be human specific and not to pick up the signal from mouse ALT
Would CSB-E10082h serve the purpose?

A:
Thanks for your inquiry.
Reviewing from your research purpose, human ALT is the reasonable consideration.Our elisa kits #CSB-E10082h is for determination for content,not for detectiono of enzyme activity.
Strictly speaking,if the concentrate of ALT locates in the detection range of kit #CSB-E10082h ,it can be detected out.Meanwhile,a point need to pay attention,as your sample is from serum of mice,then there would be a matrix problem.
And reviewing from your description,there will be supersession when injecting the natural ALT (human liver ALT) or transcription by other ways into mice.
Based on comprehensive analysis,you can consider to do pre-testing.Pls let me know if you have any further questions. Thank you.
Additionally,you need to do further validation on how to evaluate whether the modeling of humanized mice is successful or not.Pls let me know if you have any further questions. Thank you.
Q:

May I know the cross reactivity about CSB-E10082h. Can you help us to check and revert?Does the human ALT ELISA Kit have any cross reaction with mouse ALT?

A:
Thanks for your inquiry.
#CSB-E10082h,Human Alanine Aminotransferase,ALT/GPT ELISA Kit
We checked and the above kit has no cross reactions with either mouse ALT or rat ALT.Pls let me know if you have any further questions. Thank you.
Q:

If the kit is free of human or animal byproducts, HIV/AIDS/HCV/HBV/infectious material.

A:
Thanks for your inquiry. The kit is developed for the human matrix to quantitatively detect the content of the corresponding protein.
Some components of the kit contain BSA,there is no HIV / AIDS / HCV / HBV / infective material.
All kits are for scientific research use, there is no infectious component and can be used in accordance with conventional biosecurity rules of operation. Pls let me know if you have any further questions. Thank you.

Target Data

Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Gene References into Functions
  1. a relationship between NAFLD with high ALT and lower BMD in the general U.S. population PMID: 29897928
  2. The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
  3. In men with hepatic steatosis, we found that elevated serum ALT levels were associated with lower serum SHBG levels. PMID: 28540984
  4. Our results using Mendelian randomization suggest that ALT reduces the risk of ischaemic heart disease (IHD), probably through reducing triglyceride levels. PMID: 28007909
  5. Measurements of serum alpha-fetoprotein (AFP) levels could be helpful in detecting progression or recurrence in treated patients with hepatitis B virus (HBV) - hepatocellular carcinoma (HCC) and normal alanine aminotransferase (ALT). PMID: 27304617
  6. Baseline alanine transaminase (ALT) and a hepatitis B surface antigen (HBsAg) above 1000 IU/ml were independent predictive factors for ALT elevation. PMID: 27935953
  7. High serum alanine aminotransferase expression is associated with metabolic syndrome. PMID: 27390880
  8. The data of this study confirmed the genetic heterogeneity of childhood-onset pure HSP, with SPG4/SPAST and SPG3A/ATL1 being the most frequent forms. PMID: 27260292
  9. Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
  10. Liver fat content and serum ALT were inversely correlated with bone mineral density in middle-aged and elderly men. PMID: 26791408
  11. Age, female gender, and elevated ALT can help identifying cases with biliary etiology of acute pancreatitis. PMID: 26664483
  12. IBS [irritable bowel syndrome ] may be an important condition in certain patients with elevated ALT levels and MS [ metabolic syndrome ]. PMID: 26632395
  13. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GPT1 rises due to up-regulation of hepatic expression of GPT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
  14. Elevated aminotransferase levels, even within the normal range, were significantly associated with increased risk of all external-cause mortalities, including suicide, and injury. PMID: 26446640
  15. There are significant relationships of elevated ALT levels with cardiometabolic risk factors (obesity, hypertension, hypercholesteremia, hyperuricemia, hypertriglyceridemia) and several sex-related differences in rural Chinese. PMID: 26160405
  16. ALT was significantly associated with elevated levels of triacylglycerols, hyperglycemia, and obesity. In patients with metabolic syndrome, one must consider liver involvement if the patient has ALT levels in the upper third of the reference range. PMID: 25874513
  17. Peginterferon alfa-2a is associated with elevations in alanine aminotransferase at the end of treatment in chronic hepatitis C patients with sustained virologic response. PMID: 24937007
  18. A prospective association between ALT levels and metabolic syndrome highlights the value of ALT levels, even mild ALT elevations within the normal range, as a predictor of the MetS risk in male schizophrenia patients. PMID: 24819732
  19. Serum glutamate pyruvate transaminase levels were elevated postoperatively in recipients of an orthotopic liver transplant from a brain-dead donor receiving extracorporeal membrane oxygenation. PMID: 25543901
  20. Statistically significant differences were observed in the mean values of GPT in type 1 diabetics compared to type 2 and control groups PMID: 24849491
  21. Alanine aminotransferase showed associations with BMI, glycaemia, lipids, and creatinine. The relationship between alanine aminotransferase and age was best expressed by polynomial regression. PMID: 24953204
  22. Serum alanine aminotransferase has limited predictive value for liver disease in chronic hepatitis C PMID: 25219834
  23. Role of insulin resistance and adipocytokines on serum alanine aminotransferase in obese patients with type 2 diabetes mellitus PMID: 23884827
  24. In nonalcoholic fatty liver disease, adipose tissue insulin resistance (but not hepatic insulin resistance) and liver triglyceride content are major factors in the elevation of plasma aminotransferase levels. PMID: 25145475
  25. elevated ALT is common in the Korean population; it is associated with metabolic disorders (obesity, insulin resistance, or metabolic syndrome) in the majority of patients PMID: 24275711
  26. ALT and visceral adipose tissue affect metabolic risk factors in a middle-aged Japanese population PMID: 24500143
  27. PNPLA3 I148M polymorphism is associated with elevated alanine transaminase levels in Mexican indigenous and Mestizo populations. PMID: 24691744
  28. Among patients with chronic HCV infection, daily consumption of filtered coffee may have a beneficial effect on the stabilization of ALT levels. PMID: 24349501
  29. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
  30. Elevated ALT is associated with developing depressive symptoms, suggesting that non-alcoholic fatty liver disease may represent an independent modifiable risk factor for depression. PMID: 23522007
  31. fibrosis is not rare in Chinese CHB patients with persistent normal alanine aminotransferase, especially HBeAg-positive patients over 30 years old PMID: 24205292
  32. Elevated serum ALT levels are prevalent among elderly agricultural and fishing population workers in Taiwan. PMID: 24158044
  33. Elevated serum alanine aminotransferase level was independently associated with an increased risk of intracerebral hemorrhage in East Asian men. PMID: 23880909
  34. Normal to near-normal serum aminotransferase levels are associated with metabolic disorders. PMID: 23794308
  35. Data from cross-sectional study in children/adolescents in Republic of Korea suggest normal ALT/GPT serum reference values; ALT/GPT serum level appears effective as biomarker in predicting cardiovascular/metabolic syndrome risk in population studied. PMID: 23403445
  36. Among HIV/HCV-coinfected persons, risky drinking was associated with a higher adjusted mean AST (62.2 vs. 51.4 U/L; adjusted ratio of means 1.2, 95% CI [1.07, 1.37], p = .003) and ALT (51.3 vs. 41.6 U/L; compared with non-risky drinking PMID: 23384374
  37. Nonalcoholic fatty liver disease and metabolic syndrome were associated with ALT levels in a male population from southern China. PMID: 22975640
  38. ALT[Alanine transaminase] may be a marker of an underlying etiology relating to the paradoxical associations of diabetes and IHD at a population level. PMID: 22954960
  39. An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold. PMID: 22389715
  40. In a cross-sectional analysis of Taiwanese industrial employees, elevated ALT associated with MS, but in subjects who did not meet MS criteria, elevated ALT by itself did not associate with IFG/T2DM. PMID: 21715038
  41. Patients with HLA-DQA1 and DRB1 genotypes have higher alanine aminotransferase elevation, whereas HLA-negative cases have a higher incidence of Gilbert's syndrome. PMID: 22357454
  42. In a cross-sectional study, Chinese adolescents and young adults with more metabolic syndrome components were more likely to suffer from elevated ALT PMID: 22177542
  43. alanine aminotransferase levels largely within the normal range, there was an inverse association between ALT and all-cause mortality and cardiovascular events PMID: 22158663
  44. the COMT polymorphism affects serum ALT activity to maintain liver function PMID: 22293393
  45. The magnitude of the association of PNPLA3 with liver enzymes is driven by the size of abdominal fat. PMID: 22140488
  46. discriminates persons infected with hepatitis C virus from those at low risk of liver disease, but would be considered elevated in a large proportion of the U.S. population PMID: 21987480
  47. An hepatitis B virus DNA level of >/=5 log10 copies/mL and an alanine aminotransferase (ALT) level of >20 to PMID: 21692932
  48. The prevalence of metabolic syndrome (MS) and risk factors for and association with elevated alanine aminotransferase (ALT) as markers of hepatic injury in a large Hispanic health disparity cohort with high rates of obesity, was studied. PMID: 21720553
  49. Subjects with metabolic syndrome had significantly higher plasma levels of alanine aminotransferase. PMID: 21040936
  50. Nrmal levels of ALT are associated with metabolic and androgenic phenotypes, i women of reproductive age. PMID: 20961180

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Subcellular Location Cytoplasm
Protein Families Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Database Links

HGNC: 4552

OMIM: 138200

KEGG: hsa:2875

STRING: 9606.ENSP00000378408

UniGene: Hs.103502

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