Recombinant Human Alanine aminotransferase 1(GPT)

Code CSB-EP009864HU
Size US$1726
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names GPT
Uniprot No. P24298
Research Area Signal Transduction
Alternative Names AAT1; Alanine aminotransferase 1; Alanine aminotransferase; ALAT1_HUMAN; ALT1; Glutamate pyruvate transaminase 1; Glutamic alanine transaminase 1; Glutamic pyruvate transaminase (alanine aminotransferase); Glutamic pyruvic transaminase 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT 1; gpt; GPT1
Species Homo sapiens (Human)
Source E.coli
Expression Region 2-496aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 58.5kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Gene References into Functions
  1. a relationship between NAFLD with high ALT and lower BMD in the general U.S. population PMID: 29897928
  2. The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
  3. In men with hepatic steatosis, we found that elevated serum ALT levels were associated with lower serum SHBG levels. PMID: 28540984
  4. Our results using Mendelian randomization suggest that ALT reduces the risk of ischaemic heart disease (IHD), probably through reducing triglyceride levels. PMID: 28007909
  5. Measurements of serum alpha-fetoprotein (AFP) levels could be helpful in detecting progression or recurrence in treated patients with hepatitis B virus (HBV) - hepatocellular carcinoma (HCC) and normal alanine aminotransferase (ALT). PMID: 27304617
  6. Baseline alanine transaminase (ALT) and a hepatitis B surface antigen (HBsAg) above 1000 IU/ml were independent predictive factors for ALT elevation. PMID: 27935953
  7. High serum alanine aminotransferase expression is associated with metabolic syndrome. PMID: 27390880
  8. The data of this study confirmed the genetic heterogeneity of childhood-onset pure HSP, with SPG4/SPAST and SPG3A/ATL1 being the most frequent forms. PMID: 27260292
  9. Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
  10. Liver fat content and serum ALT were inversely correlated with bone mineral density in middle-aged and elderly men. PMID: 26791408
  11. Age, female gender, and elevated ALT can help identifying cases with biliary etiology of acute pancreatitis. PMID: 26664483
  12. IBS [irritable bowel syndrome ] may be an important condition in certain patients with elevated ALT levels and MS [ metabolic syndrome ]. PMID: 26632395
  13. Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GPT1 rises due to up-regulation of hepatic expression of GPT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
  14. Elevated aminotransferase levels, even within the normal range, were significantly associated with increased risk of all external-cause mortalities, including suicide, and injury. PMID: 26446640
  15. There are significant relationships of elevated ALT levels with cardiometabolic risk factors (obesity, hypertension, hypercholesteremia, hyperuricemia, hypertriglyceridemia) and several sex-related differences in rural Chinese. PMID: 26160405
  16. ALT was significantly associated with elevated levels of triacylglycerols, hyperglycemia, and obesity. In patients with metabolic syndrome, one must consider liver involvement if the patient has ALT levels in the upper third of the reference range. PMID: 25874513
  17. Peginterferon alfa-2a is associated with elevations in alanine aminotransferase at the end of treatment in chronic hepatitis C patients with sustained virologic response. PMID: 24937007
  18. A prospective association between ALT levels and metabolic syndrome highlights the value of ALT levels, even mild ALT elevations within the normal range, as a predictor of the MetS risk in male schizophrenia patients. PMID: 24819732
  19. Serum glutamate pyruvate transaminase levels were elevated postoperatively in recipients of an orthotopic liver transplant from a brain-dead donor receiving extracorporeal membrane oxygenation. PMID: 25543901
  20. Statistically significant differences were observed in the mean values of GPT in type 1 diabetics compared to type 2 and control groups PMID: 24849491
  21. Alanine aminotransferase showed associations with BMI, glycaemia, lipids, and creatinine. The relationship between alanine aminotransferase and age was best expressed by polynomial regression. PMID: 24953204
  22. Serum alanine aminotransferase has limited predictive value for liver disease in chronic hepatitis C PMID: 25219834
  23. Role of insulin resistance and adipocytokines on serum alanine aminotransferase in obese patients with type 2 diabetes mellitus PMID: 23884827
  24. In nonalcoholic fatty liver disease, adipose tissue insulin resistance (but not hepatic insulin resistance) and liver triglyceride content are major factors in the elevation of plasma aminotransferase levels. PMID: 25145475
  25. elevated ALT is common in the Korean population; it is associated with metabolic disorders (obesity, insulin resistance, or metabolic syndrome) in the majority of patients PMID: 24275711
  26. ALT and visceral adipose tissue affect metabolic risk factors in a middle-aged Japanese population PMID: 24500143
  27. PNPLA3 I148M polymorphism is associated with elevated alanine transaminase levels in Mexican indigenous and Mestizo populations. PMID: 24691744
  28. Among patients with chronic HCV infection, daily consumption of filtered coffee may have a beneficial effect on the stabilization of ALT levels. PMID: 24349501
  29. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
  30. Elevated ALT is associated with developing depressive symptoms, suggesting that non-alcoholic fatty liver disease may represent an independent modifiable risk factor for depression. PMID: 23522007
  31. fibrosis is not rare in Chinese CHB patients with persistent normal alanine aminotransferase, especially HBeAg-positive patients over 30 years old PMID: 24205292
  32. Elevated serum ALT levels are prevalent among elderly agricultural and fishing population workers in Taiwan. PMID: 24158044
  33. Elevated serum alanine aminotransferase level was independently associated with an increased risk of intracerebral hemorrhage in East Asian men. PMID: 23880909
  34. Normal to near-normal serum aminotransferase levels are associated with metabolic disorders. PMID: 23794308
  35. Data from cross-sectional study in children/adolescents in Republic of Korea suggest normal ALT/GPT serum reference values; ALT/GPT serum level appears effective as biomarker in predicting cardiovascular/metabolic syndrome risk in population studied. PMID: 23403445
  36. Among HIV/HCV-coinfected persons, risky drinking was associated with a higher adjusted mean AST (62.2 vs. 51.4 U/L; adjusted ratio of means 1.2, 95% CI [1.07, 1.37], p = .003) and ALT (51.3 vs. 41.6 U/L; compared with non-risky drinking PMID: 23384374
  37. Nonalcoholic fatty liver disease and metabolic syndrome were associated with ALT levels in a male population from southern China. PMID: 22975640
  38. ALT[Alanine transaminase] may be a marker of an underlying etiology relating to the paradoxical associations of diabetes and IHD at a population level. PMID: 22954960
  39. An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold. PMID: 22389715
  40. In a cross-sectional analysis of Taiwanese industrial employees, elevated ALT associated with MS, but in subjects who did not meet MS criteria, elevated ALT by itself did not associate with IFG/T2DM. PMID: 21715038
  41. Patients with HLA-DQA1 and DRB1 genotypes have higher alanine aminotransferase elevation, whereas HLA-negative cases have a higher incidence of Gilbert's syndrome. PMID: 22357454
  42. In a cross-sectional study, Chinese adolescents and young adults with more metabolic syndrome components were more likely to suffer from elevated ALT PMID: 22177542
  43. alanine aminotransferase levels largely within the normal range, there was an inverse association between ALT and all-cause mortality and cardiovascular events PMID: 22158663
  44. the COMT polymorphism affects serum ALT activity to maintain liver function PMID: 22293393
  45. The magnitude of the association of PNPLA3 with liver enzymes is driven by the size of abdominal fat. PMID: 22140488
  46. discriminates persons infected with hepatitis C virus from those at low risk of liver disease, but would be considered elevated in a large proportion of the U.S. population PMID: 21987480
  47. An hepatitis B virus DNA level of >/=5 log10 copies/mL and an alanine aminotransferase (ALT) level of >20 to PMID: 21692932
  48. The prevalence of metabolic syndrome (MS) and risk factors for and association with elevated alanine aminotransferase (ALT) as markers of hepatic injury in a large Hispanic health disparity cohort with high rates of obesity, was studied. PMID: 21720553
  49. Subjects with metabolic syndrome had significantly higher plasma levels of alanine aminotransferase. PMID: 21040936
  50. Nrmal levels of ALT are associated with metabolic and androgenic phenotypes, i women of reproductive age. PMID: 20961180
  51. liver enzymes, especially ALT, were significantly associated with IR according to direct clamp assessment, which were independent of the traditional risk factors in diabetic patients PMID: 20972737
  52. Data suggest that serum alanine aminotransferase is inadequate for diagnosing lamivudine resistance and has implications where viral resistance testing is suboptimal and for reimbursement of rescue therapy. PMID: 20872970
  53. Lowering the ALT upper normal reference limit will facilitate earlier detection of risky phenotypes of obesity. PMID: 20433554
  54. Chronic hepatitis B patients with high non-specific cytotoxic T-Lymphocytes also has the high ALT level. PMID: 20387481
  55. No significant relationship between serum hepatitis B virus DNA level and liver histological stage, gender, age, body mass index, or hepatitis B surface antigen was observed in these patients with normal ALT. PMID: 20190678
  56. All metabolic syndrome-related risk factors are significantly correlated with the increase in blood levels of alanine aminotransferase in a population of Japanese men and women. PMID: 19687577
  57. elevation in GPT in obese children most likely reflects insulin resistance PMID: 20032584
  58. Obesity and elevated ALT are associated with insulin resistance PMID: 19938201
  59. Glucose intolerance is associated with elevated serum aminotransferase independent of obesity, but even a mildly elevated ALT is relatively uncommon in free-living men with glucose intolerance. PMID: 12480555
  60. Histology of nonalcoholic fatty liver disease in individuals with normal ALT values, not significantly different from those with elevated ALT levels, and low normal value does not guarantee freedom from steatohepatitis with advanced fibrosis. PMID: 12774006
  61. genotypes of aldehyde dehydrogenase 2 and beta3-adrenergic receptor were strongly associated with elevated alanine aminotransferase level which increased with the accumulation of components of metabolic syndrome PMID: 14506613
  62. In coinfected Hic/hepatitis C patients, normal ALT levels are not an indicator of mild necroinflammation and may not portend a more benign disease course. PMID: 16652026
  63. In obesity, elevated ALT activity is associated with increased fasting insulin suggesting that insulin resistance, rather than body mass index alone, plays a role in mediating the increased aminotransferase activity. PMID: 17134955
  64. Provides a quantitative analysis of the main impact of the trauma of liver resection, liver ischemia, and other factors on the postoperative evolution of transaminases. PMID: 17378732
  65. Insulin resistance in obese patients is associated with alanine aminotransferase activity. PMID: 17405346
  66. Data show that OCT/ALT is a potent indicator for the diagnosis and the prognosis of hepatocellular carcinoma. PMID: 17570354
  67. May serve as a useful marker for non-alcoholic fatty liver disease or diabetes risk in Filipino women. PMID: 17764776
  68. Weight reduction secondary to a hypocaloric diet were associated with improvement in hypertransaminasemia and insulin resistance in nonalcoholic fatty liver disease. PMID: 17766001
  69. In hepatitis C, alterations in the liver tissue as reflected by ALT elevation are mainly associated with periportal bridging/necrosis, viral load and duration of disease. PMID: 17907292
  70. Type 2 diabetic patients have 80% more liver fat than age-, weight-, and sex-matched nondiabetic subjects, which is underestimated in type 2 diabetics. PMID: 17934148
  71. In obese patients with NAFLD (nonalcoholic fatty liver disease), OSA (obstructive sleep apnea) was associated with elevated alanine aminotransferase levels and a trend toward histologic evidence of progressive liver disease. PMID: 18090161
  72. ALAT elevations associate with obesity and diabetes, and provide new evidence that in elderly male patients, ALAT elevations, absent other known liver diseases, may selectively associate with diabetes PMID: 18178283
  73. Data revealed higher alanine aminotransferase for women with a family history of diabetes, when adjusted for age and BMI. PMID: 18242760
  74. Raised ALT is common in apparently healthy British South Asians, and is significantly associated with an adverse metabolic and atherothrombotic risk profile. PMID: 18315552
  75. Non-alcoholic fatty liver disease is the most common cause of persistently elevated serum alanine aminotransferase level among the general population of Iran. PMID: 18473412
  76. Tag-free ALT1 and ALT2 were obtained by cleavage of enterokinase digestion and used for initial characterization of the enzymes. The specific ALT activity of purified fusion or His-tag-removed ALT1 was about 15-fold higher than that of ALT2 PMID: 18508279
  77. found a strong relationship between alanine aminotransferase levels and metabolic syndrome in metabolic syndrome PMID: 18763311
  78. HEV markers (HEV RNA and anti-HEV) were detected in donors with elevated ALT levels who were widely distributed over Japan PMID: 18774966
  79. Serum levels are an independent risk factor for the development of type 2 diabetes mellitus in subjects without fatty liver or hepatic dysfunction. PMID: 19065605
  80. In the US population, elevated gamma-glutamyltransferase (GGT) was associated with mortality from all causes, liver disease, cancer, and diabetes, while alanine aminotransferase (ALT) was associated only with liver disease mortality. PMID: 19100265
  81. alanine aminotransferase may have a role in progression of cardiovascular disease and diabetes in Koreans PMID: 19159884
  82. A high proportion of obese children present elevated ALT1 levels. PMID: 19217453
  83. High ALT level and low platelet count are closely associated with the development of hepatocarcinogenesis in hepatitis C carrirs. who are thus candidates for antiviral agents. PMID: 19232448
  84. approximately 60% of the variation in S-ALT, a marker of liver fat content, is genetically determined PMID: 19303161
  85. A clinical method for selective measurement of ALT1 and 2 in human plasma is described. PMID: 19360321

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Subcellular Location Cytoplasm
Protein Families Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Tissue Specificity Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Database Links

HGNC: 4552

OMIM: 138200

KEGG: hsa:2875

STRING: 9606.ENSP00000378408

UniGene: Hs.103502

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