Human Histone H4(HIST1H4A) ELISA kit

Code CSB-EL010429HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P62805
Lead Time 7-14 working days
Abbreviation HIST1H4A
Target Name histone cluster 1, H4a
Alias H4/a, H4FA, H4 histone family, member A|histone 1, H4a
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 37.5 pg/mL-2400 pg/mL
Sensitivity 9.3 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 101  
Range % 95-108  
1:2 Average % 88  
Range % 83-99  
1:4 Average % 89  
Range % 87-92  
1:8 Average % 110  
Range % 106-115  
Recovery
The recovery of human HIST1H4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 89-97  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2400 2.757 2.657 2.707 2.539  
1200 2.024 1.924 1.974 1.806  
600 1.226 1.146 1.186 1.018  
300 0.665 0.655 0.660 0.492  
150 0.390 0.380 0.385 0.217  
75 0.281 0.271 0.276 0.108  
37.5 0.225 0.215 0.220 0.052  
0 0.173 0.163 0.168    
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Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H4 family
Database Links

HGNC: 4781

OMIM: 142750

KEGG: hsa:121504

STRING: 9606.ENSP00000367034

UniGene: Hs.143080

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