Code | CSB-EL010429HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 101 | ||||
Range % | 95-108 | |||||
1:2 | Average % | 88 | ||||
Range % | 83-99 | |||||
1:4 | Average % | 89 | ||||
Range % | 87-92 | |||||
1:8 | Average % | 110 | ||||
Range % | 106-115 |
The recovery of human HIST1H4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-97 | ||||
EDTA plasma (n=4) | 94 | 90-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human HIST1H4A ELISA Kit was designed for the quantitative measurement of Human HIST1H4A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 37.5 pg/mL-2400 pg/mL and the sensitivity is 9.3 pg/mL.
Sample type: Protein (recombinant/purified)(Bovine mixed histones were dissolved in PBS and plasma respectively)
Sample species: Bovine
Sample dilution: No dilution
Review: I used CSB-EL010429HU to measure the concentration of bovine mixed histones, and the standard curve fitted by a four-parameter model yielded a R2 of 0.99771 (see picture). The standard curve met the expectations and the OD values ranged from 0 to 2.75.
By Anonymous