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Human Proto-oncogene Wnt-1(WNT1) ELISA kit

Citations (3) Protocol MSDS
Code CSB-EL026128HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
wingless-type MMTV integration site family, member 1
Alternative Names
BMND16 ELISA Kit; INT1 ELISA Kit; OI15 ELISA Kit; oncogene Int1 ELISA Kit; Proto oncogene protein Wnt 1 ELISA Kit; Proto-oncogene Int-1 homolog ELISA Kit; Proto-oncogene Wnt-1 ELISA Kit; Wingless type MMTV integration site family member 1 ELISA Kit; wingless-type MMTV integration site family; member 1 (oncogene INT1) ELISA Kit; Wnt 1 ELISA Kit; wnt1 ELISA Kit; WNT1_HUMAN ELISA Kit
Abbreviation
WNT1
Uniprot No.
P04628
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Stem Cells
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human WNT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 87-92  
1:2 Average % 97  
Range % 94-103  
1:4 Average % 101  
Range % 97-106  
1:8 Average % 88  
Range % 82-94  
Recovery
The recovery of human WNT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 89-94  
EDTA plasma (n=4) 99 96-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.499 2.659 2.579 2.415  
10 2.145 2.001 2.073 1.909  
5 1.402 1.522 1.462 1.298  
2.5 0.944 0.950 0.947 0.783  
1.25 0.666 0.645 0.656 0.492  
0.625 0.422 0.454 0.438 0.274  
0.312 0.289 0.295 0.292 0.128  
0 0.158 0.169 0.164    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

WNT1, also known as wingless-type MMTV integration site family member 1, is a secreted signaling glycoprotein that plays an essential role in embryonic development and cellular communication. As a member of the Wnt signaling pathway, WNT1 regulates cell fate determination, proliferation, and differentiation through both canonical and non-canonical signaling cascades. This proto-oncogene is particularly important in neural development. It has been implicated in various pathological conditions, making it a significant target for research in developmental biology, cancer studies, and regenerative medicine.

The Human Proto-oncogene Wnt-1 ELISA kit (CSB-EL026128HU) is designed for quantitative measurement of WNT1 in human serum, plasma, and tissue homogenates using a sandwich assay principle. The kit offers a detection range of 0.312 ng/mL to 20 ng/mL with a sensitivity of 0.078 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection is performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research examining Wnt signaling pathways and their role in various biological processes. Studies have used the kit to measure Wnt1 protein levels in different sample types including serum, plasma, and cell lysates from differentiated human embryonic stem cells.

• Cancer research: Plasma Wnt1 measurements in patients receiving immune checkpoint inhibitor therapy to evaluate treatment responses and monitor signaling pathway changes
• Developmental biology: Quantification of Wnt1 protein levels in cell lysates from human embryonic stem cell-derived organoid-like structures during differentiation processes
• Biomarker studies: Serum-based Wnt1 protein detection for potential clinical biomarker applications in disease monitoring and therapeutic evaluation
• Cell signaling research: Analysis of Wnt1 expression patterns in controlled cell culture systems to understand pathway regulation and cellular differentiation mechanisms

Citations

Related Products

WNT1 Antibodies

WNT1 Proteins

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Target Background

Function
(From Uniprot)
Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Plays an essential role in the development of the embryonic brain and central nervous system (CNS). Has a role in osteoblast function, bone development and bone homeostasis.
Gene References into Functions
  1. MiR301a increased the radiosensitivity and inhibited the migration of radioresistantESCC cells by targeting WNT1. PMID: 30365079
  2. The WNT1 mutation disrupts feedback regulation between these miRNAs and WNT1, providing insights into the pathogenesis of WNT-related bone disorders. These miRNAs may have potential in the diagnosis and treatment of osteoporosis. PMID: 29506076
  3. Taking together, these results suggest that Wnt/beta-catenin signal pathway activation-dependent up-regulation of syncytin-1 contributes to the pro-inflammatory factor TNF-alpha-enhanced fusion between oral squamous cell carcinoma cells and endothelial cells. PMID: 28112190
  4. High WNT1 expression is associated with metastasis in cholangiocarcinoma. PMID: 30193944
  5. We conclude that severe form of osteogenesis imperfecta (OI) may be associated with WNT1 mutation. This novel variant of WNT1 identified in achild with OI is likely cause for the disease. PMID: 29481978
  6. MiR-185 inhibits colon cancer cell proliferation and invasion by targeting Wnt1. PMID: 29454608
  7. High WNT1 expression is associated with epithelial-mesenchymal transition in gastric cancer. PMID: 29273006
  8. Biallelic pathogenic splice acceptor site variant (c.359-3C>G) in WNT1 gene located on chromosome 12q13.12 underlying osteogenesis imperfecta type 3 in a Pakistani family. PMID: 28665926
  9. Vertebral compression fractures were present in 78% (7/9) of those aged over 50years but were not seen in younger mutation-positive subjects. All those with fractures had several severely compressed vertebrae. Altogether spinal compression fractures were present in 39% of those with a WNT1 mutation. Only PMID: 28411110
  10. miR-218 promoted the apoptosis of human ovarian carcinoma cells via suppression of the WNT/beta-catenin signaling pathway. PMID: 28900081
  11. Data suggest that brain imaging be performed in any individual with WNT1-associated osteogenesis imperfecta (OI) who also has developmental delay or any neurological deficits. PMID: 26671912
  12. Data indicate a tetracyclic azafluorenone, SJ26, that is capable of binding to G-quadruplex DNA structure, repressing WNT1 expression, and inhibiting cell migration. PMID: 27626678
  13. Regulation of WNT signaling by VSX2 during optic vesicle patterning in human induced pluripotent stem cells has been described. PMID: 27301076
  14. High WNT1 expression is associated with metastasis in triple-negative breast cancer. PMID: 26967387
  15. WNT1 osteoporosis causes significant skeletal changes already in early childhood and impairs bone mass gain during pubertal years. PMID: 27005318
  16. High WNT1 expression is associated with esophageal squamous cell carcinoma. PMID: 29054489
  17. High WNT1 expression in stromal cells is associated with myelodysplastic syndrome. PMID: 28684528
  18. High WNT1 expression is associated with gastric cancer. PMID: 28223537
  19. Novel compound heterozygous WNT1 missense mutations, p.Glu123Asp and p.Cys153Gly, identified in a patient with osteogenesis imperfecta. PMID: 28528193
  20. Osteocyte protein expression is altered in patients with osteoporosis caused by WNT1 mutation. PMID: 28379384
  21. High WNT1 expression is associated with glioma cell invasion. PMID: 27006177
  22. bone formation is under the control of WNT1 produced by osteocytes, the cells that reside deep in the bone matrix and form dendritic networks. PMID: 28628035
  23. Knocking down of Wnt-1 by siRNA had the similar effect of miRNA-148a overexpression on cell migration and invasion in lung cancer cells. PMID: 28199399
  24. High WNT1 expression is associated with Lung Adenocarcinoma Progression. PMID: 27197183
  25. WNT1 overexpression is associated with Breast Cancer Invasion and Metastasis. PMID: 27197202
  26. PKA signaling is pivotal in pigmentation process itself in cultured melanoma cells, while the importance of Wnt/beta-catenin signaling should be emphasized in the context of development and differentiation PMID: 27567978
  27. Patients with WNT1 mutation-related osteoporosis respond to teriparatide treatment. PMID: 27732335
  28. High WNT1 expression is associated with osteosarcoma. PMID: 27665868
  29. These results suggest that alphaTAT1-mediated Wnt1 expression via microtubule acetylation is important for colon cancer progression. PMID: 27836544
  30. Upregulation of WNT is associated with prostate cancer. PMID: 28098905
  31. we introduced WNT1 gene as one of the important genes in Hepatocellular Carcinoma, and also, we showed that miR-122 can regulate WNT1 gene expression PMID: 27687586
  32. Promoter hypermethylation of Wnt1 may play a crucial role in lung cancer carcinogenesis. PMID: 27930522
  33. Overexpression of WNT1 is associated with hepatocellular carcinoma. PMID: 27465549
  34. High WNT expression is associated with pancreatic cancer PMID: 27485116
  35. The study expanded : Osteogenesis imperfecta (OI) spectrum and confirmed moderate to severe bone fragility induced by WNT1 defects. PMID: 27450065
  36. Wnt1 expression was significantly higher in cutaneous squamous cell carcinoma samples than in the skin of control subjects. Wnt1 expression correlated with histopathological differentiation in cutaneous squamous cell carcinoma. PMID: 27420949
  37. our results suggest that miR-148a can suppress the migration and invasion of breast cancer cells by targeting WNT-1 and inhibiting Wnt/beta-catenin signaling pathway and this will provide new insights into the molecular mechanisms of breast cancer metastasis PMID: 26707142
  38. MCF7 cells over-expressing both WNT1 and FGF3 show a 3.5-fold increase in mammosphere formation; conditioned media from these cells also promotes stem cell activity in untransfected parental MCF7 and T47D cells, as WNT1 and FGF3 are secreted factors. PMID: 26421711
  39. IWR-1 has the potential to suppress tumor metastasis by inhibiting Wnt/beta-catenin pathway as well as survivin expression PMID: 26450645
  40. High expression of WNT1 is associated with liver cancer. PMID: 26499380
  41. Studies indicate crosstalk between Notch receptor and Wnt protein, Hedgehog protein, hypoxia and transforming growth factor beta (TGFbeta)/bone morphogenetic protein (BMP) pathways. PMID: 26592459
  42. Results suggested that Wnt1-induced MAFK expression promoted cell proliferation in MG63 cells, and that the role of MAFK in osteosarcoma might be closely linked to the Wnt signaling pathway. PMID: 26214410
  43. Suggest that TGF-beta1/Smad and Wnt/CTNNB1 signaling pathways both contribute to pathological scar formation. PMID: 26261683
  44. Data suggest that fibulin-5 functions as a metastasis suppressor in lung cancer by modulating tumor microenvironment to suppress Wnt/beta-catenin signaling. PMID: 25909283
  45. Paracrine WNT signaling is stimulated by estrogen and progesterone, while autocrine WNT signaling is induced by the embryonic T-box transcription factor TBX3 PMID: 25350852
  46. Wnt1 promotes CD36 expression via activation of PPAR-gamma and TCF4. PMID: 25721714
  47. Agonists of epoxyeicosatrienoic acids reduce infarct size and ameliorate cardiac dysfunction via activation of HO-1 and Wnt1 canonical pathway. PMID: 25677507
  48. WNT1 was a direct target of miR-148b, and overexpressed WNT1 inversely correlated with miR-148b levels in hepatocellular carcinoma tissues. PMID: 25627001
  49. WNT/beta-catenin pathway and E-cadherin are important factors in advanced epithelial ovarian cancer. PMID: 24499657
  50. Wnt1 stimulates osteogenic differentiation and mineralization of human periodontal ligament fibroblasts, mainly by activating the canonical Wnt/beta-catenin pathway, in which Runx2 is a key downstream regulator. PMID: 25618247

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Involvement in disease
Osteoporosis (OSTEOP); Osteogenesis imperfecta 15 (OI15); Osteogenesis imperfecta 14 (OI14)
Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Database Links

HGNC: 12774

OMIM: 164820

KEGG: hsa:7471

STRING: 9606.ENSP00000293549

UniGene: Hs.248164

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