Human apolipoprotein E (Apo-E) ELISA Kit

Instructions
Code CSB-E09748h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name apolipoprotein E
Alternative Names AD2, LDLCQ5, LPG, MGC1571, apolipoprotein E3
Abbreviation APOE
Uniprot No. P02649
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.62 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 86-95  
1:200 Average % 102  
Range % 97-107  
1:400 Average % 91  
Range % 85-97  
1:800 Average % 97  
Range % 91-103  
Recovery
The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
1000 0.088 0.090 0.089    
500 0.135 0.142 0.139    
250 0.227 0.237 0.232    
125 0.324 0.341 0.333    
62.5 0.583 0.598 0.591    
31.25 0.847 0.864 0.856    
15.62 1.228 1.235 1.232    
0 2.155 2.199 2.177    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Involvement in disease Hyperlipoproteinemia 3 (HLPP3); Alzheimer disease 2 (AD2); Sea-blue histiocyte disease (SBHD); Lipoprotein glomerulopathy (LPG); Familial hypercholesterolemia (FH)
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, a
Database Links

HGNC: 613

OMIM: 104310

KEGG: hsa:348

STRING: 9606.ENSP00000252486

UniGene: Hs.654439

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