Human apolipoprotein E (Apo-E) ELISA Kit

Code CSB-E09748h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02649
Lead Time 3-5 working days
Abbreviation APOE
Protein Biological Process 1 Apolipoprotein
Target Name apolipoprotein E
Alias AD2, LDLCQ5, LPG, MGC1571, apolipoprotein E3
Species Homo sapiens (Human)
Protein Biological Process 3 Lipid transport
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.62 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
HGNC 613
RGD 2138
MGI 88057
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 86-95  
1:200 Average % 102  
Range % 97-107  
1:400 Average % 91  
Range % 85-97  
1:800 Average % 97  
Range % 91-103  
Recovery
The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
1000 0.088 0.090 0.089    
500 0.135 0.142 0.139    
250 0.227 0.237 0.232    
125 0.324 0.341 0.333    
62.5 0.583 0.598 0.591    
31.25 0.847 0.864 0.856    
15.62 1.228 1.235 1.232    
0 2.155 2.199 2.177    
References
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Function Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Involvement in disease Hyperlipoproteinemia 3 (HLPP3); Alzheimer disease 2 (AD2); Sea-blue histiocyte disease (SBHD); Lipoprotein glomerulopathy (LPG); Familial hypercholesterolemia (FH)
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, a
Database Links

HGNC: 613

OMIM: 104310

KEGG: hsa:348

STRING: 9606.ENSP00000252486

UniGene: Hs.654439

Pathway Cholesterol metabolism

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