APOE Antibody

Datasheet
Code CSB-PA07599A0Rb
See More Details Free Antibody trial simple
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Uniprot No. P02649
Image
  • Immunofluorescence staining of HepG2 cells with CSB-PA07599A0Rb at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC image of CSB-PA07599A0Rb diluted at 1:1000 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Apolipoprotein E protein (21-110AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:1000-1:2000, IF:1:200-1:500
Relevance Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Apolipoprotein E (Apo-E), APOE
Immunogen Species Human
Research Area Neuroscience
Target Names APOE
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
Involvement in disease Hyperlipoproteinemia 3 (HLPP3); Alzheimer disease 2 (AD2); Sea-blue histiocyte disease (SBHD); Lipoprotein glomerulopathy (LPG); Familial hypercholesterolemia (FH)
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, a
Database Links

HGNC: 613

OMIM: 104310

KEGG: hsa:348

STRING: 9606.ENSP00000252486

UniGene: Hs.654439

Pathway Cholesterol metabolism

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