Human heat shock protein 90α (HSP-90α) ELISA kit

Code CSB-E13462h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07900
Lead Time 3-5 working days
Abbreviation HSP-90α
Protein Biological Process 1 Neurobiology
Target Name heat shock protein 90α (HSP-90α)
Alias FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, a
Species Homo sapiens (Human)
Protein Biological Process 3 Stress response
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 5253
RGD 631409
MGI 96250
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-90α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %93
Range %90-95
1:10Average %104
Range %100-107
1:20Average %94
Range %89-97
1:40Average %99
Range %92-103
Recovery
The recovery of human HSP-90α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-97
EDTA plasma (n=4)9491-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.712 2.699 2.706 2.569
252.013 2.002 2.008 1.871
12.51.400 1.422 1.411 1.274
6.250.804 0.788 0.796 0.659
3.120.424 0.428 0.426 0.289
1.560.349 0.341 0.345 0.208
0.780.245 0.234 0.240 0.103
00.139 0.135 0.137  
References
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Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function
Subcellular Location Nucleus, Cytoplasm, Melanosome, Cell membrane
Protein Families Heat shock protein 90 family
Database Links

HGNC: 5253

OMIM: 140571

KEGG: hsa:3320

STRING: 9606.ENSP00000335153

UniGene: Hs.525600

Pathway Estrogen signaling pathway
PI3K-Akt signaling pathway
Necroptosis
Protein processing in endoplasmic reticulum
Antigen processing and presentation
IL-17 signaling pathway
NOD-like receptor signaling pathway
Th17 cell differentiation

Q&A and Customer Reviews

Q&A
Q:

Can you please tell me if your kit CSB-E13462h, is specific to HSP-90 alpha only? If so, do you have evidence of this?

A:
Thanks for your inquiry.
CSB-E13462h
This kit is designed according to: https://www.uniprot.org/uniprot/P07900.
It is specific to detect heat shock protein 90α (HSP-90α). The specificity of the raw materials for this kit have been screened and validated strictly. So we can guarantee the specificity of the kit.
Here is the link for the reference of this kit.
http://journals.sagepub.com/doi/abs/10.1177/1073274818804489?cookieSet=1
Pls let me know if you have any further questions. Thank you.
Customer Reviews

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