Human secretory immunoglobulin A,SIgA ELISA Kit

Code CSB-E09235h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human SIgA ELISA Kit was designed for the quantitative measurement of Human SIgA protein in saliva, urine, tear. It is a Sandwich ELISA kit, its detection range is 0.012 μg/mL-50 μg/mL and the sensitivity is 0.012 μg/mL.

Target Name secretory immunoglobulin A,SIgA
Abbreviation SIgA
Species Homo sapiens (Human)
Sample Types saliva, urine, tear
Detection Range 0.012 μg/mL-50 μg/mL
Sensitivity 0.012 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average Corrected  
50 2.207 2.320 2.264 2.193  
12.5 2.104 2.234 2.169 2.098  
3.125 1.855 1.846 1.851 1.780  
0.781 1.539 1.551 1.545 1.474  
0.195 0.775 0.801 0.788 0.717  
0.049 0.416 0.401 0.409 0.338  
0.012 0.192 0.196 0.194 0.123  
0 0.069 0.072 0.071    
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
  • Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.
  • One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.
  • One vial Stop Solution (10ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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