Human secretory immunoglobulin A,SIgA ELISA Kit

Code CSB-E09235h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Target Name
secretory immunoglobulin A,SIgA
Homo sapiens (Human)
Sample Types
saliva, urine, tear
Detection Range
0.012 μg/mL-50 μg/mL
0.012 μg/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average Corrected  
50 2.207 2.320 2.264 2.193  
12.5 2.104 2.234 2.169 2.098  
3.125 1.855 1.846 1.851 1.780  
0.781 1.539 1.551 1.545 1.474  
0.195 0.775 0.801 0.788 0.717  
0.049 0.416 0.401 0.409 0.338  
0.012 0.192 0.196 0.194 0.123  
0 0.069 0.072 0.071    
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
  • Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.
  • One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.
  • One vial Stop Solution (10ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

The human secretory immunoglobulin A (SIgA) ELISA kit is a valuable tool for detecting and quantifying SIgA levels in human saliva, urine, and tears. With a detection range spanning from 0.012 μl/mL to 50 μl/mL and a sensitivity of 0.012 μl/mL, it offers a precise measurement of SIgA levels. The assay uses a sandwich method for measurement. The assay time typically ranges from 1 to 5 hours, requiring a sample volume of 50-100 μl. The detection wavelength is at 450 nm, ensuring accurate and reliable results. This ELISA kit has been utilized in several research articles, showcasing its effectiveness and relevance in the field of immunology and biomarker analysis.

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We are planning a study to detect levels of salivary IgA antibodies in dental caries active children ( 5-10 years). Can you provide approprivate ELISA kits for our study?

We would like to recommend CSB-E09235h. The Human secretory immunoglobulin A,SIgA ELISA Kit is recommnended for the quantitative detection of human IgA in saliva, urine, tear. Please find protocol at:
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