| Description |
This Human SIgA ELISA Kit was designed for the quantitative measurement of Human SIgA protein in saliva, urine, tear. It is a Sandwich ELISA kit, its detection range is 0.012 μg/mL-50 μg/mL and the sensitivity is 0.012 μg/mL. |
| Target Name |
secretory immunoglobulin A,SIgA |
| Abbreviation |
SIgA |
| Species |
Homo sapiens (Human) |
| Sample Types |
saliva, urine, tear |
| Detection Range |
0.012 μg/mL-50 μg/mL |
| Sensitivity |
0.012 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| μg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.207 |
2.320 |
2.264 |
2.193 |
|
| 12.5 |
2.104 |
2.234 |
2.169 |
2.098 |
|
| 3.125 |
1.855 |
1.846 |
1.851 |
1.780 |
|
| 0.781 |
1.539 |
1.551 |
1.545 |
1.474 |
|
| 0.195 |
0.775 |
0.801 |
0.788 |
0.717 |
|
| 0.049 |
0.416 |
0.401 |
0.409 |
0.338 |
|
| 0.012 |
0.192 |
0.196 |
0.194 |
0.123 |
|
| 0 |
0.069 |
0.072 |
0.071 |
|
|
|
|
| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
- Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.
- One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.
- One vial Stop Solution (10ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
|
| ELISA Data Analysis |
ELISA Standard Curve & Curve Expert software |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
