Rat secretory immunoglobulin A,sIgA ELISA Kit

Rat secretory immunoglobulin A,sIgA ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Rat secretory immunoglobulin A,sIgA ELISA Kit

Name: Rat secretory immunoglobulin A,sIgA ELISA Kit
Brand: CUSABIO
SKU: CSB-E08412r
Price: 150 USD
Availability: InStock
Rating: 5.0 (1 reviews)

Citations (6) Protocol MSDS

Code	CSB-E08412r
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Product Details
Citations (6)
Related Products
Customer Reviews and Q&A

Target Name

secretory immunoglobulin A,sIgA

Abbreviation

sIgA

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.78 ng/mL-50 ng/mL

Sensitivity

0.195 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	97-106
1:200	Average %	90
	Range %	86-96
1:400	Average %	97
	Range %	92-103
1:800	Average %	88
	Range %	84-92

Recovery

The recovery of rat sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.511	2.371	2.441	2.302
25	2.046	1.976	2.011	1.872
12.5	1.512	1.501	1.507	1.368
6.25	0.979	1.003	0.991	0.852
3.12	0.482	0.518	0.500	0.361
1.56	0.334	0.359	0.347	0.208
0.78	0.241	0.229	0.235	0.096
0	0.143	0.135	0.139

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat sIgA ELISA Kit was designed for the quantitative measurement of Rat sIgA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

Combined Bacterial Translocation and Cholestasis Aggravates Liver Injury by Activation Pyroptosis in Obstructive Jaundice X Shen,SSRN,2023
Effects of prebiotics in combination with probiotics on intestinal hydrolase activity, microbial population and immunological biomarkers in SD rats fed an AIN-93G diet MJ Kim,Laboratory animal research,2022
Impact of winter savory extract (Satureja montana L.) on biochemical parameters in serum and oxidative status of liver with application of the principal component analysis in extraction solvent selection B Milijašević,European review for medical and pharmacological sciences,2022
Lycopene Modulates Placental Health and Fetal Development Under High‐Fat Diet During Pregnancy of Rats S Sun,Molecular Nutrition & Food Research,2021
Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats Jian Zhou.et al,British Journal of Nutrition,2018
Modulating Effect of Peptide Therapy on the Morphofunctional State of Bronchial Epithelium in Rats with Obstructive Lung Pathology. Kuzubova NA.et al,Bull Exp Biol Med.,2015

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Serum

Sample species: Rat

Sample dilution: No dilution

Review: I used CSB-E08412R to detect the content of HMGB1 in colon and lung tissue homogenates. The product is convenient and fast, with high sensitivity. The experimental results are reliable, the detection results are reliable, and the trend is obvious. It is recommended to use.

By Anonymous

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.