Mouse secretory immunoglobulin A,sIgA ELISA Kit

Instructions
Code CSB-E08413m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name secretory immunoglobulin A,sIgA
Abbreviation sIgA
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 92  
Range % 87-95  
1:40 Average % 101  
Range % 97-104  
1:80 Average % 93  
Range % 87-97  
1:160 Average % 98  
Range % 92-102  
Recovery
The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-97  
EDTA plasma (n=4) 96 92-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
1000 2.486 2.466 2.476 2.312  
500 1.772 1.841 1.807 1.643  
250 1.232 1.222 1.227 1.063  
125 0.760 0.748 0.754 0.590  
62.5 0.464 0.481 0.473 0.309  
31.2 0.325 0.312 0.319 0.155  
15.6 0.239 0.229 0.234 0.070  
0 0.166 0.162 0.164    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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