Chicken secretory immunoglobulin A,SIgA ELISA Kit

Code CSB-E10097Ch
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Chicken SIgA ELISA Kit was designed for the quantitative measurement of Chicken SIgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.234 μg/mL-60 μg/mL and the sensitivity is 0.058 μg/mL.

Target Name secretory immunoglobulin A,SIgA
Abbreviation SIgA
Species Gallus gallus (Chicken)
Sample Types serum, plasma
Detection Range 0.234 μg/mL-60 μg/mL
Sensitivity 0.058 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 86  
Range % 82-89  
1:400 Average % 98  
Range % 92-104  
1:800 Average % 97  
Range % 93-101  
1:1600 Average % 88  
Range % 84-92  
The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 104 98-108  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
60 0.345 0.346 0.346    
15 0.565 0.593 0.579    
3.75 0.856 0.872 0.864    
0.94 1.158 1.197 1.178    
0.234 1.367 1.289 1.328    
0 1.881 1.825 1.853    
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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For the kit CSB-E10097Ch,may I know if it can be tested with tissue samples, specifically small intestine?
Would you have this information available to share?

Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.
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