Chicken secretory immunoglobulin A,SIgA ELISA Kit

Chicken secretory immunoglobulin A,SIgA ELISA Kit

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Citations

Customer Reviews and Q&A

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Product Details

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Customer Reviews and Q&A

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Chicken secretory immunoglobulin A,SIgA ELISA Kit

Name: Chicken secretory immunoglobulin A,SIgA ELISA Kit
Brand: CUSABIO
SKU: CSB-E10097Ch

Citations (4) Protocol

Code	CSB-E10097Ch
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations (4)
Related Products
Customer Reviews and Q&A

Description

This Chicken SIgA ELISA Kit was designed for the quantitative measurement of Chicken SIgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.234 μg/mL-60 μg/mL and the sensitivity is 0.058 μg/mL.

Target Name

secretory immunoglobulin A,SIgA

Abbreviation

SIgA

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma

Detection Range

0.234 μg/mL-60 μg/mL

Sensitivity

0.058 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	86
	Range %	82-89
1:400	Average %	98
	Range %	92-104
1:800	Average %	97
	Range %	93-101
1:1600	Average %	88
	Range %	84-92

Recovery

The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	104	98-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
60	0.345	0.346	0.346
15	0.565	0.593	0.579
3.75	0.856	0.872	0.864
0.94	1.158	1.197	1.178
0.234	1.367	1.289	1.328
0	1.881	1.825	1.853

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Effects of dietary supplementation of trans-anethole on the intestinal antioxidant status, immune function, and liver lipid metabolism in broilers C Yu,Italian Journal of Animal Science,2022
The effects and combinational effects of Bacillus subtilis and montmorillonite on the intestinal health status in laying hens JF Chen,Poultry Science,2020
Montmorillonite improved the intestinal mucosal barrier functions of laying hens in late production Chen JF,Journal of Animal Physiology and Animal Nutrition,2019
Effects of dietary essential oil and enzyme supplementation on growth performance and gut health of broilers challenged by Clostridium perfringens Qiujuan Sun. et al,Animal Feed Science and Technology,2015

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■ Q&A

For the kit CSB-E10097Ch,may I know if it can be tested with tissue samples, specifically small intestine?
Would you have this information available to share?

Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.