Pig secretory immunoglobulin A,SIgA ELISA Kit

Pig secretory immunoglobulin A,SIgA ELISA Kit

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Pig secretory immunoglobulin A,SIgA ELISA Kit

Name: Pig secretory immunoglobulin A,SIgA ELISA Kit
Brand: CUSABIO
SKU: CSB-E12063p

^Instructions

Code	CSB-E12063p
Size	96T,5×96T,10×96T
Trial Size
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Target Name

secretory immunoglobulin A,SIgA

Abbreviation

sIgA

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.15 μg/mL-37.5 μg/mL

Sensitivity

0.15 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-97
1:400	Average %	94
	Range %	90-98
1:800	Average %	87
	Range %	85-94
1:1600	Average %	96
	Range %	91-103

Recovery

The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	103	98-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
37.5	0.120	0.115	0.118
9.38	0.456	0.463	0.460
2.34	0.901	0.877	0.889
0.58	1.432	1.527	1.480
0.15	2.103	2.189	2.146
0	2.544	2.753	2.649

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Effects of stocking density on growth performance, blood parameters and immunity of growing pigs X Li,Animal Nutrition,2020
Modulation of Pectin on Mucosal Innate Immune Function in Pigs Mediated by Gut Microbiota W Wu,Microorganisms,2020
Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets Xiong X.et al,J Anim Sci.,2015
Dietary supplementation with yeast product improves intestinal function, and serum and ileal amino acid contents in weaned piglets Xia Xiong.et al,Livestock Science,2015
Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012
Kadar imunoglobulin A sekretori pada penderita tonsilitis kronik sebelum dan setelah tonsilektomi Indo Sakka et al,Oto Rhino Laryngologica Indonesiana ,2011

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■ Q&A

Could you indicate me if your kit could be valid for feces samples? Any recommendations for this type of samples?

Thanks for your inquiry.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.