Pig secretory immunoglobulin A,SIgA ELISA Kit

Code CSB-E12063p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name secretory immunoglobulin A,SIgA
Abbreviation sIgA
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.15 μg/mL-37.5 μg/mL
Sensitivity 0.15 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:200 Average % 92  
Range % 88-97  
1:400 Average % 94  
Range % 90-98  
1:800 Average % 87  
Range % 85-94  
1:1600 Average % 96  
Range % 91-103  
The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 103 98-108  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
37.5 0.120 0.115 0.118    
9.38 0.456 0.463 0.460    
2.34 0.901 0.877 0.889    
0.58 1.432 1.527 1.480    
0.15 2.103 2.189 2.146    
0 2.544 2.753 2.649    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Could you indicate me if your kit could be valid for feces samples? Any recommendations for this type of samples?

Thanks for your inquiry.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.


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