Bovine secretory immunoglobulin A,sIgA ELISA Kit

Code CSB-E11192b
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Bovine sIgA ELISA Kit was designed for the quantitative measurement of Bovine sIgA protein in saliva. It is a Competitive ELISA kit, its detection range is 2.4 μg/mL-150 μg/mL and the sensitivity is 2.4 μg/mL.

Abbreviation sIgA
Species Bos taurus (Bovine)
Sample Types saliva
Detection Range 2.4 μg/mL-150 μg/mL
Sensitivity 2.4 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of bovine sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 92  
Range % 85-101  
1:40 Average % 95  
Range % 90-99  
1:80 Average % 93  
Range % 85-97  
1:160 Average % 97  
Range % 92-110  
The recovery of bovine sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-100  
EDTA plasma (n=4) 94 87-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
150 0.243 0.247 0.245    
75 0.381 0.409 0.395    
37.5 0.565 0.558 0.562    
18.75 0.804 0.792 0.798    
9.4 0.999 1.004 1.002    
4.7 1.270 1.259 1.265    
2.4 1.569 1.621 1.595    
0 2.515 2.336 2.426    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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