Human soluble OX40L (sOX40L) ELISA kit

Code CSB-E12752h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P23510
Lead Time 3-5 working days
Abbreviation TNFSF4
Target Name tumor necrosis factor (ligand) superfamily, member 4
Alias CD134L, CD252, GP34, OX-40L, OX4OL, TXGP1, CD134 ligand|OX40 antigen ligand|tax-transcriptionally activated glycoprotein 1 (34kD)
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF4/OX4. It is found to be involved in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD40-stimulated B cells, this cytokine along with CD70 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.
HGNC 11934
RGD 619816
MGI 104511
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sOX40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %106
Range %100-113
1:2Average %100
Range %95-105
1:4Average %89
Range %82-98
1:8Average %97
Range %92-102
Recovery
The recovery of human sOX40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-98
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.554 2.603 2.579 2.450
51.975 2.065 2.020 1.891
2.51.305 1.402 1.354 1.225
1.250.744 0.714 0.729 0.600
0.6250.476 0.498 0.487 0.358
0.3120.351 0.326 0.339 0.210
0.1560.227 0.226 0.227 0.098
00.132 0.126 0.129  
 
References
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Function Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Involvement in disease Systemic lupus erythematosus (SLE)
Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Tumor necrosis factor family
Database Links

HGNC: 11934

OMIM: 152700

KEGG: hsa:7292

STRING: 9606.ENSP00000281834

UniGene: Hs.181097

Pathway Target Therapy

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