Human soluble OX40L (sOX40L) ELISA kit

Instructions
Code CSB-E12752h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tumor necrosis factor (ligand) superfamily, member 4
Alternative Names CD 134L ELISA Kit; CD 252 ELISA Kit; CD134 ligand ELISA Kit; CD134L ELISA Kit; CD252 ELISA Kit; CD252 antigen ELISA Kit; glycoprotein 34 kd ELISA Kit; Glycoprotein Gp34 ELISA Kit; GP34 ELISA Kit; OX-40L ELISA Kit; OX40 antigen ligand ELISA Kit; OX40 ligand ELISA Kit; OX40L ELISA Kit; TAX transcriptionally-activated glycoprotein 1 ELISA Kit; TNFL4_HUMAN ELISA Kit; Tnfsf4 ELISA Kit; Tumor necrosis factor (ligand) superfamily member 4 ELISA Kit; Tumor necrosis factor ligand superfamily member 4 ELISA Kit; TXGP1 ELISA Kit
Abbreviation TNFSF4
Uniprot No. P23510
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human sOX40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %106
Range %100-113
1:2Average %100
Range %95-105
1:4Average %89
Range %82-98
1:8Average %97
Range %92-102
Recovery
The recovery of human sOX40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-98
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.554 2.603 2.579 2.450
51.975 2.065 2.020 1.891
2.51.305 1.402 1.354 1.225
1.250.744 0.714 0.729 0.600
0.6250.476 0.498 0.487 0.358
0.3120.351 0.326 0.339 0.210
0.1560.227 0.226 0.227 0.098
00.132 0.126 0.129  
 
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Gene References into Functions
  1. It is concluded that the rs3850641 SNP is significantly associated with the breast cancer susceptibility in Iranian population. PMID: 29949525
  2. TNFSF4 SNPs rs844648, rs844648 and rs704840 were associated with an increased risk of neuromyelitis optica spectrum disorders in Chinese population. PMID: 29032462
  3. The study suggests that the TNFSF4 gene may be involved in the susceptibility to Vogt-Koyanagi-Harada disease and Behcet's Disease in Han Chinese PMID: 27872495
  4. present study suggests a novel association between specific TNFSF4 and BLK gene polymorphisms and allergic rhinitis risk. PMID: 28713926
  5. Data suggest that tumour necrosis factor superfamily 4 (TNFSF4) rs704840 could be the potential systemic lupus erythematosus (SLE) risk factors in the Malaysian population. PMID: 27519474
  6. House dust mite sublingual immunotherapy downregulated Th2-type immune responses mediated by the TSLP-OX40L signaling pathway in patients with persistent moderate to severe allergic rhinitis. PMID: 27012942
  7. OX40 and OX40L formed a functional complex, which may facilitate signal transduction from OX40L to OX40 and contribute to the pathogenesis of Grave's disease. PMID: 27107937
  8. The polymorphisms of the TNFSF4 gene may contribute to the susceptibility to pathogenesis of early-Onset Autoimmune Thyroid Diseases and Hypothyroidism of Hashimoto's Thyroiditis. PMID: 27556446
  9. the SNPs in TNFSF4 and FAM167A-BLK may be involved in asthma and allergic rhinitis gene risk in the Han Chinese cohort. PMID: 27088737
  10. Given the discovery cohort, functional data, and importance of TNFSF4 in infection clearance, TNFSF4C may associate with outcomes and warrants future studies. PMID: 26348892
  11. Review/Meta-analysis: TNFSF4 (rs3850641) polymorphisms is not associated with coronary heart disease risk. PMID: 27008001
  12. Study showed that the A allele of the rs7518045 and haplotype rs3861950C-rs17346501C-rs7518045A-rs1234313G in the TNFSF4 gene were associated with decreased risk for myocardial infarction in a Chinese Han population. PMID: 26125814
  13. higher expression in patients with nasal polyposis compared with patients with chronic rhinosinusitis without nasal polyps PMID: 24583911
  14. contributes to the aberrant T Follicular Helper response in systemic lupus erythematosus PMID: 26070486
  15. Data indicate that soluble OX40 and sOX40L plasma levels are increased in early rheumatoid arthritis patients. PMID: 25359291
  16. Report role of TNFSF4 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
  17. There were no significant associations between rs1234313, rs1234314 and rs17568 and atherosclerotic cerebral infarction risk in a Han Chinese population. PMID: 24595151
  18. Blocking of the interaction between OX40 and OX40L in vitro inhibited production of Thl and Th2 type cytokines in acute renal allograft rejection. PMID: 23953582
  19. TNFSF4 gene polymorphism rs3861950, but not rs3850641, is associated with the risk of atherosclerosis CI in a Chinese population. PMID: 23184501
  20. The results of a meta-analysis suggest that the TNFSF4 rs2205960 polymorphism may confer susceptibility to systemic lupus erythematosus (SLE) in different populations and that the TNFSF4 rs1234315 polymorphism is associated with susceptibility to SLE in Asians. PMID: 22850862
  21. Our data firstly replicated the association of TNFSF4 with renal disorder in SLE patients in the Chinese population, which supported that TNFSF4 may act as a marker of lupus nephritis PMID: 23936824
  22. Our data confirm a global signal at TNFSF4 and a role for the expressed product at multiple stages of lymphocyte dysregulation during SLE pathogenesis PMID: 23874208
  23. The expression levels of OX40 and OX40L were higher in pSS patients. PMID: 23948416
  24. TNFSF4 may have little significance as a common genetic component of Sjogren's syndrome and primary biliary cirrhosis in the Chinese Han population. PMID: 23622253
  25. OX40L expression can be used as a marker to evaluate antiviral treatment efficacy and extend the notion that enhancement of OX40L expression could be a good way for immunotherapy in chronic hepatitis C patients. PMID: 23589118
  26. results suggest that the rs3850641 and rs17568 polymorphisms in the OX40L and OX40 genes are associated with some of the lipid and lipoprotein variations in subjects with endogenous HTG PMID: 23216302
  27. findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction PMID: 22646697
  28. The simultaneous expression of both OX40 and its ligand OX40L in idiopathic inflammatory myopathies suggests that they might participate in disease pathogenesis PMID: 23469620
  29. data primarily indicates that rs3850641 of OX40L gene contributes to sporadic breast carcinogenesis in a northeast Chinese Han population. PMID: 22870213
  30. This meta-analysis confirms that TNFSF4 polymorphisms are associated with susceptibility to systemic lupus erythematosus in Asians and Europeans PMID: 22820624
  31. We confirm TNFSF4 as an systemic sclerosis susceptibility gene and rs2205960 as a putative causal variant with preferential association in the ACA+ subphenotype. PMID: 22422496
  32. Concentrations of sOX40L were found to be high in adult asthmatic patients and were associated with the severity of the disease. Therefore, sOX40L could be a potential infl ammatory mediator in the pathogenesis of asthma. PMID: 22760717
  33. Augmentation of OX40-OX40L signals in interferon type I receptor (IFNalphaR)-deficient transgenic mice can substantially enhance CD4+ T cell, but not CD8+ T cell primary responses. PMID: 22156349
  34. High OX40L is associated with acute coronary syndrome and stable angina. PMID: 21986129
  35. Up-regulation of OX40L may play a critical role in development of childhood atopic asthma and correlates with asthma severity. PMID: 21781408
  36. OX40 gene polymorphism may be associated with a risk of ACS in the Han Chinese population, although the association between OX40L polymorphisms and ACS requires further investigation PMID: 21476935
  37. data indicate that the TNFSF4 rs45454293T-allele is associated with lower TNFSF4 expression and increased risk of myocardial infarction PMID: 21445270
  38. Both OX40 upregulation and sOX40L increase were closely associated with Henoch-Schonlein purpura (HSP), especially HSP with nephritis. PMID: 21143648
  39. These results demonstrate for the first time that HDAC11 plays an essential role in regulating OX40L expression. PMID: 21239696
  40. Our study not only suggested that the TNFSF4 gene was associated with systemic lupus erythematosus in the Chinese Han population, but also implied that the TNFSF4 gene might also predispose multiple populations to systemic lupus erythematosus. PMID: 20012871
  41. Data show that MMP-2-conditioned dendritic cells primed naive CD4(+) T cells to differentiate into an inflammatory T(H)2 phenotype through OX40L expression and inhibition of IL-12p70 production. PMID: 21397857
  42. TNFSF4 is a susceptibility gene of coronary heart disease in Chinese Han population PMID: 21402531
  43. The data confirm the influence of TNFSF4 polymorphisms in systemic sclerosis genetic susceptibility, especially in subsets of patients positive for lcSSc and anti-centromere antibodies. PMID: 21187296
  44. our data provide an in vivo role for the OX40/OX40L system in the innate immune response during polymicrobial sepsis PMID: 20844189
  45. Polymorphisms in the TNFSF4 gene region are associated with susceptibility to systemic sclerosis and its clinical and autoantibody subsets. PMID: 19778912
  46. OX40/OX40L expression is increased in the bronchial submucosa in mild asthma, but not in moderate-to-severe disease, and is related to the degree of tissue eosinophilia and IL-4 expression. PMID: 20139223
  47. Expression of CD134 and CD134 ligand in lesional and nonlesional psoriatic skin. PMID: 12624783
  48. combined use of a vector driving the expression of OX40L with three other costimulatory molecules (B7-1, ICAM-1, and LFA-3) both enhances initial activation and then further potentiates sustained activation of nai;ve and effector T cells. PMID: 12798307
  49. An OX40L-dependent mechanism is demonstrated in plasmacytoid dendritic cell-mediated T helper cell responses; OX40L selectively induces Th2-type immune responses by promoting CD4+ T cells to secrete IL-4, IL-5, and IL-13. PMID: 15034038
  50. Elucidation of cross-talk between OX40 and OX40L could be very important in understanding interaction of cells present in inflamed airways of asthma. PMID: 15100674
  51. an increasing amount of gp34/OX40L expression leads to an increasing level of up-regulation of the allogeneic CD4+ T-cell response by dendritic cells PMID: 15218969
  52. Activated human natural killer (NK) cells are able to help T-cell receptor-stimulated autologous CD4+ T cells by a process that involves both OX40 ligand and B7 antigen costimulation. PMID: 15356117
  53. T cell proliferation by direct cross-talk between OX40 ligand on human mast cells and OX40 on human T cells. PMID: 15470070
  54. Single Nucleotide polymorphism in TNFS4 increase the risk of Arteriosclerosis PMID: 15750594
  55. OX40L costimulates human antiviral memory CD8 T cell responses largely through indirect effects and can enhance anti-influenza, anti-EBV, and anti-HIV responses, particularly in combination with 4-1BBL or B7. PMID: 16272289
  56. OX40L on thymic stromal lymphopoietin-activated dendritic cells triggers T helper type 2 (Th2) cell polarization in the absence of IL-12, and can switch IL-10-producing regulatory Th cell responses into TNF-alpha-producing inflammatory Th cell responses. PMID: 16275760
  57. OX40 ligand (OX40L) completely inhibited the generation of IL-10-producing CD4(+) type 1 regulatory T (Tr1) cells from naive and memory CD4(+) T cells induced by the immunosuppressive drugs dexamethasone and vitamin D3. PMID: 16924108
  58. the quantitation of sOX40L was correlated with the age and among these subjects, those of 70s and 80s have much higher sOX40L concentration than those of 60s PMID: 17166734
  59. The demonstrate rapid infiltration of activated (OX40(+)) CD4(+) T cells into HSV-1-infected corneas and expression of OX40L on MHC Class II-negative cells, which are present in the infected corneas and required for HSK. PMID: 17185358
  60. study findings indicate that functional OX40L is inducible on human activated CD4+ and CD8+ T cells, and that the expression is enhanced by TGF-beta1 PMID: 17584577
  61. TSLP-induced molecule on dendritic cells that triggers inflammatory T(H)2 differentiation in the absence of IL-12. Review. PMID: 17666213
  62. OX40 ligand (OX40L) is a critical in vivo mediator of TSLP-mediated Th2 responses. PMID: 18060034
  63. Our present findings, if corroborated in other prospective investigations, suggest that the TNFSF4 variants tested may be useful indicators for assessing the risk of venous thromboembolism. PMID: 18356244
  64. Results show that the OX40-OX40L interaction suppresses IL-17 production by PHA-stimulated human PBMC and purified CD4 and CD8 cells. PMID: 18501882
  65. Patients with ACS show increased OX40L system (pOX40L and sOX40L) expression which may create a proinflammatory milieu for aggravating the development of atherosclerosis, and may be a valuable marker for predicting the severity of ACS. PMID: 18674525
  66. human primary T cells are programmed to rapidly express functional OX40L molecules after stimulation under DNA-damaging conditions, demonstrating that the induction of OX40L by T cells is independent of cell proliferation. PMID: 18718855
  67. thymic stromal lymphopoietin (TSLP) and human DCs plays an essential role in evoking inflammatory Th2 responses in allergy through OX40 ligand expression on DCs. PMID: 18832690
  68. Study shows that TNFSF4 is expressed on antigen-presenting cells in human carotid atherosclerotic lesions but provides no evidence for an association of TNFSF4 gene variation with the risk for ischemic stroke. PMID: 18998106
  69. Dendritic cells matured in the presence of PGE(2) induced the expression of OX40, OX40L, and CD70 on T cells facilitating T-cell/T-cell interaction that warrant long-lasting costimulation. PMID: 19029446
  70. allele frequencies and genotype distributions of TNFSF4 SNPs were not substantially different between the control group and the myocardial infarction group. PMID: 19029970
  71. role in systemic lupus erythematosus (Review) PMID: 19083191
  72. This first replication study confirms the association of genetic variation in the upstream region of TNFSF4 with susceptibility to SLE. PMID: 19092840
  73. Study confirmed the associations of BANK1 (rs3733197)and TNFSF4 (rs844648) with systemic lupus erythematosus in Hong Kong Chinese. PMID: 19357697
  74. indicate a distinct CTL effector function in response to intracellular pathogens triggered via differing endogenous IL-2 production upon costimulation through CD252 PMID: 19494280
  75. OX40L may play an important role in the early phase of T cell activation and proliferation PMID: 19663699

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Involvement in disease Systemic lupus erythematosus (SLE)
Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Tumor necrosis factor family
Database Links

HGNC: 11934

OMIM: 152700

KEGG: hsa:7292

STRING: 9606.ENSP00000281834

UniGene: Hs.181097

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