Mouse Interferon α1,IFN-α1 ELISA Kit

Code CSB-E08638m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse Ifna1 ELISA Kit was designed for the quantitative measurement of Mouse Ifna1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Target Name Interferon α1,IFN-α1
Alternative Names
Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit; IFN-alpha-1 ELISA Kit
Abbreviation Ifna1
Uniprot No. P01572
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-α1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %90-102
1:2Average %90
Range %82-99
1:4Average %101
Range %83-107
1:8Average %98
Range %93-105
Recovery
The recovery of mouse IFN-α1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-95
EDTA plasma (n=4)10293-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
2002.493 2.489 2.491 2.353
1001.770 1.799 1.785 1.647
501.164 1.122 1.143 1.005
250.745 0.768 0.757 0.619
12.50.382 0.397 0.390 0.252
6.250.273 0.293 0.283 0.145
3.120.220 0.232 0.226 0.088
00.137 0.139 0.138  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Gene References into Functions
  1. These findings also identify STAT3 as a therapeutic target against viral infection and highlight it as an essential pathway component for endogenous and therapeutic IFN-alpha responsiveness. PMID: 27988795
  2. the close association between the increased proportion of CD180-negative B cells and the activation of IFN-alpha signaling in Systemic lupus erythematosus, is reported. PMID: 26277892
  3. IL-12 and IFN-alpha differentially program CD8 T cells to re-express distinct levels of PD-1 upon re-encountering Ag, resulting in IL-12-stimulated cells being less susceptible to exhaustion in the face of sustained tumor Ag. PMID: 23804712
  4. The expansion of CD8 T cells depends on type I IFN, type I IFN and IL-12 or is largely independent of the two cytokines. PMID: 22815848
  5. A spontaneous genomic duplication and frameshift mutation in the guanine exchange factor dedicator of cytokinesis 2 (Dock2) that has arisen in at least a subset of circulating Irf5(-/-) mice and inadvertently been bred to homozygosity. PMID: 22431588
  6. Knockdown of endogenous guanylate binding protein (GBP)4 increases IRF7-mediated IFN-alpha production, whereas overexpression of GBP4 has the opposite effect. PMID: 22095711
  7. protects against antigen-induced arthritis PMID: 21469099
  8. Data show that similar T cell expansion and serum IgG responses were observed in adenovirus (Adv)-IFN-treated WT and BAFF-deficient mice despite their disparate pathological and clinical responses. PMID: 21383240
  9. Studies in both mice and humans have demonstrated a role for IFN-alpha/beta in directly influencing the fate of both CD4(+) and CD8(+) T cells during the initial phases of antigen recognition. PMID: 21320124
  10. IFN-alpha enhances both the induction and maintenance of programmed cell death (PD)-1 expression on T cell receptor-engaged primary mouse T cells through association of IFN-responsive factor 9 (IRF9) with the IFN stimulation response element. PMID: 21263073
  11. This article reports the production of interferon alpha/beta (IFN-alpha/beta) by SJL/J mouse brain astrocyte cultures infected with Theiler's murine encephalomyelitis virus (TMEV). PMID: 20038206
  12. TLR ligands facilitate antibody response by inducing type I interferon (IFN), which in turn elicits rapid and significant amounts of antigen-specific IgG2c. PMID: 20566717
  13. These results demonstrate that mouse hepatitis virus is recognized by both RIG-I and MDA5 and induces IFN-alpha/beta through the activation of the IRF-3 signaling pathway. PMID: 20427526
  14. Sufficient IFN-alpha production by plasmacytoid dentritic cells is an important determinant of vaccine efficacy. PMID: 20065291
  15. B cell-mediated type I IFN induction depended on the transcription factor IRF3. PMID: 17925397
  16. The involvement of IRF1 and IRF2 in TLR2-mediated responses, was studied. PMID: 18652890
  17. Report that tumor-targeted interferon-alpha1 delivery by Tie2-expressing monocytes inhibits tumor growth and metastasis. PMID: 18835032
  18. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes. PMID: 19120292
  19. Collectively, these findings demonstrate that constitutive and LPS-induced type I IFN play significant roles in regulating the differences in phenotype and function between BMM and GM-BMM. PMID: 19406830
  20. Recombinant IFN-alpha treatment promotes monocyte chemoattractant protein (MCP)-1, MCP-5, and, to a lesser extent, MCP-3 production in the bone marrow during murocytomegalovirus infection. PMID: 19620305

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Subcellular Location Secreted.
Protein Families Alpha/beta interferon family
Database Links

KEGG: mmu:15962

STRING: 10090.ENSMUSP00000092580

UniGene: Mm.57127

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