Rat C-Reactive Protein,CRP ELISA Kit

Instructions
Code CSB-E07922r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name C-reactive protein, pentraxin-related
Alternative Names Crp ELISA kit; Ptx1C-reactive protein ELISA kit
Abbreviation CRP
Uniprot No. P48199
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 31.25 ng/mL-2000 ng/mL
Sensitivity 7.81 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 89  
Range % 85-96  
1:4 Average % 93  
Range % 89-97  
1:8 Average % 99  
Range % 92-105  
Recovery
The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 88-97  
EDTA plasma (n=4) 96 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
2000 2.664 2.701 2.683 2.541  
1000 1.701 1.716 1.709 1.567  
500 1.173 1.182 1.178 1.036  
250 0.604 0.589 0.597 0.455  
125 0.398 0.372 0.385 0.243  
62.5 0.302 0.288 0.295 0.153  
31.25 0.234 0.227 0.231 0.089  
0 0.144 0.140 0.142    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Supernatant
    Sample species: Rat
    Review: Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. As shown in figure, the levels of TGF-β1, CRP, IL-6 and BNP were significantly increased in MI rats than Sham. Salvianolate treatment dose-dependently reduced these levels compared with MI rats.
    PMID: 30466624
  • Sample type: Serum
    Sample species: Rat
    Review: Renin, ANGII, C-reactive protein (CRP) and interleukin-6 (IL-6) levels were determined by ELISA. Our results advocate a positive correlation between CRP serum levels and CSA, which is indicative for vascular hypertrophy . Irrespective of the induction of hypertension, CRP may be implicated as mediator of vascular remodeling after injury
    PMID: 30021383
  • Sample type: Serum
    Sample species: Rat
    Review: Frozen aliquots of serum samples were thawed firstly on ice. Subsequently, commercial ELISA kits were used to detect the levels of interleukin-6 (IL-6), tumor necrosis factor alfa (TNF-α), C-reactive protein (CRP), adrenocorticotropic hormone (ACTH), corticotropin-releasing hormone (CRH), and corticosterone (CORT) in serum, following the instructions provided by the manufacturer. Chronic PSD for 7 days resulted in higher levels of serum pro-inflammatory cytokines including IL-6, TNF-α, and CRP with P values < 0.001, 0.01 and 0.001, respectively
    PMID: 30953642
  • Sample type: Serum
    Sample species: Rat
    Review: The levels of serum C-reactive protein (CRP) were determined with ELISA kits. Effect of HuoxueAnshen Recipe on serum CRP before and 2 h after I/R.
    PMID: 28479928
  • Sample type: Plasma
    Sample species: Rat
    Review: The levels of serum C-reactive protein (CRP) were determined with ELISA kits. The contents of plasma MDA, TNF-α, and CRP were significantly higher in the moderate and high- dose PM2.5 exposure groups than in the controls (P < .05).
    PMID: 27592525

Target Data

Function Displays several functions associated with host defense
Gene References into Functions
  1. coordinated upregulation of genes encoding CRP, interleukin-6 (IL-6), HNF1alpha, and HNF4alpha in the livers and white adipose tissue (WAT) of chronic renal failure rats, as compared to the pair-fed and control animals. PMID: 29330688
  2. CRP: varied between DJOS and SHAM groups maintained on HF/HF, CD/CD, and CD/HF. PMID: 29849871
  3. Fibrinopeptide A induces CRP expression in Vascular Smooth Muscle Cells via Reactive Oxygen Species-ERK1/2/p38-NF-kappaB signal pathway. PMID: 29768263
  4. Knockdown of ZFAS1 or miR-150 overexpression effectively relieved AMI-induced myocardial infarction in AMI-1 week rats. The ZFAS1/miR-150 axis was involved in the molecular mechanism of AMI induced cardiomyocytes apoptosis via regulating CRP PMID: 28295592
  5. Epigallocatechin-3-gallate inhibits Ang II-induced CRP generation by interfering with AT1-ROS-ERK1/2 signal pathway in hepatocytes, preventing inflammation. PMID: 27492718
  6. Salvianolic acid B exerted anti-steatotic and anti-inflammatory effects against alcoholic liver injury by inducing SIRT1-mediated inhibition of CRP and ChREBP expression. PMID: 27989594
  7. Rosiglitazone inhibits angiotensin II-induced C-reactive protein generation by interfering with AT1-ROS-ERK1/2/JNK signal pathway and up-regulating PPARgamma expression in hepatocytes PMID: 26741265
  8. These findings provide evidence that, in the presence of high CRP levels, rosuvastatin exhibits significant anti-inflammatory, anti-oxidative, and renoprotective effects. PMID: 25536316
  9. Data show that palmitic acid significantly stimulated mRNA and protein expression of C-reactive protein, inducible nitric oxide synthase and tumor necrosis factor-alpha in vascular smooth muscle cells in time- and concentration-dependent manners. PMID: 25242580
  10. No correlation was found between CRP levels and microvascular or macrovascular dysfunction in adjuvant-induced arthritis. PMID: 25708190
  11. Homocysteine initiates an inflammatory response in VSMCs by stimulating CRP production via the NMDAr-ROS-ERK1/2/p38-NF-kappaB signal pathway. PMID: 25016361
  12. CRP and IgM depositions demonstrate a parallel time course throughout the reperfusion to hepatocellular damage, inflammatory response and activated complement deposition in an ischemia-reperfusion model. PMID: 24642533
  13. These demonstrate that aldosterone is able to induce CRP expression via MR-ROS-ERK1/2-NF-kappaB signal pathway in VSMCs, which provides a new evidence for the proinflammatory and proatherosclerotic effects of aldosterone. PMID: 25109280
  14. Monomeric CRP localized with inflammatory cells in infarcted myocardium. Intravenous pCRP administration significantly enhanced leukocyte rolling, adhesion, and transmigration via localized dissociation to mCRP in inflamed cremaster muscle. PMID: 24982116
  15. In older animals, there is depressed PAP expression related to a blunted inflammatory response in acute pancreatitis which is associated with worsened bacterial infiltration and higher C-reactive protein level PMID: 22807607
  16. PDIA3 might be potentially involve into the occurence and development of acute rejection response in renal transplantation and increased plasma IL-2, IL-4, IL-6, and CRP levels play an important role to prevent acute kidney allograft rejection in rats. PMID: 22201020
  17. C-reactive protein down-regulates endothelial nitric oxide synthase expression and promotes apoptosis in endothelial progenitor cells through receptor for advanced glycation end-products PMID: 22301267
  18. After attachment of polymeric CRP to the surface of necrotic cardiomyocytes, generation of monomeric CRP was demonstrated over a period of 18 h. PMID: 21856781
  19. This finding could indicate a possible role for CRP in the progression of small vessel disease in the brain and provide a therapeutic target PMID: 21840509
  20. C-reactive protein and tumour necrosis factor-alpha are elevated in collagen-induced arthritis in Dark Agouti rats PMID: 21633874
  21. Increased levels of CRP are an early sign of alcoholic fatty liver in rats. PMID: 21806828
  22. C-reactive protein exerts a proinflammatory action via Toll-like receptor 4-dependent signaling pathway (AT(1)R-p38 MAPK-TLR4-PKCalpha) in rat vascular smooth muscle cells. PMID: 20332628
  23. CRP may cause insulin resistance by increasing IRS-1 phosphorylation at Ser(307) and Ser(612) via JNK and ERK1/2, respectively, leading to impaired insulin-stimulated glucose uptake, GLUT4 translocation, and glycogen synthesis PMID: 17279354
  24. the Ala12 allele of PPARG2 may be an independent factor associated with low serum hs-CRP levels in Japanese females PMID: 17574226
  25. levels of C-reactive protein and adiponectin are improved after exercise training in diabetic rats PMID: 17660720
  26. CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation PMID: 17981193
  27. CRP levels and cardiovascular disease risk and the long term clinical outcome after acute coronary syndrome but such correlation has yet to be proven regarding CRP and atherosclerosis. PMID: 18254451
  28. C-reactive protein stimulates superoxide anion release and tissue factor activity in vivo PMID: 18621373
  29. CRP at clinically relevant concentrations (10 to 20 microg/mL) causes a disruption of the blood-brain barrier PMID: 19246692
  30. The results suggest that CRP contributes to memory loss and early phase of pathogenesis of Alzheimer disease PMID: 19263040
  31. The results demonstrate that ET-1 induces CPR production in VSMCs via ET(A) receptor followed by ROS and MAPK signal pathway. PMID: 19748488

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Subcellular Location Secreted
Protein Families Pentraxin family
Tissue Specificity Found in plasma.
Database Links

KEGG: rno:25419

STRING: 10116.ENSRNOP00000000058

UniGene: Rn.16463

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