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| Code | CSB-E07404r |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The rat OPG ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure rat OPG in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. OPG in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated OPG antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the OPG bound in the initial step. The OPG concentration can be calculated according to the standard curve. This kit is tested with high sensitivity and strong specificity. OPG, also called TNFRSF11B, is a secreted glycoprotein central to bone turnover via its role as a decoy receptor for the RANKL. The OPG/RANK/RANKL network is involved in bone remodeling and modulates the differentiation and activation of osteoclasts and hence the critical balance between bone formation and bone resorption. OPG-RANKL inhibits RANKL/RANK interaction, thus blocking osteoclastogenesis. OPG has traditionally been linked to a number of bone-related diseases. In vitro studies indicate that OPG is expressed in cells involved in atheroma plaque development and progression, such as arterial smooth muscle cells and pulmonary artery smooth muscle cells. It also seems to play an important role on cell survival through its interaction with TRAIL. |
| Target Name | tumor necrosis factor receptor superfamily, member 11b |
| Alternative Names | Tnfrsf11b ELISA Kit; Opg ELISA Kit; Tumor necrosis factor receptor superfamily member 11B ELISA Kit; Osteoprotegerin ELISA Kit |
| Abbreviation | TNFRSF11B |
| Uniprot No. | O08727 |
| Species | Rattus norvegicus (Rat) |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 0.78 ng/ml - 50 ng/ml |
| Sensitivity | 0.195 ng/ml |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | |
| Linearity | |
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| Typical Data | |
| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
| Troubleshooting and FAQs |
ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
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| Function (From Uniprot) |
Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
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| Gene References into Functions |
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| Subcellular Location | Secreted. |
| Database Links |
KEGG: rno:25341 STRING: 10116.ENSRNOP00000011344 UniGene: Rn.202973 |
