Human Endothelin 1,ET-1 ELISA Kit

Code CSB-E07007h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05305
Lead Time 3-5 working days
Abbreviation EDN1
Target Name endothelin 1
Alias ET1, HDLCQ7, preproendothelin 1
Species Homo sapiens (Human)
Sample Types serum, cell culture supernates, urine, saliva
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
HGNC 3176
RGD 2532
MGI 95283
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 82-97  
1:2 Average % 98  
Range % 94-103  
1:4 Average % 97  
Range % 90-105  
1:8 Average % 87  
Range % 82-91  
Recovery
The recovery of human ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.722 2.752 2.737 2.628  
100 2.169 2.322 2.246 2.137  
50 1.545 1.528 1.537 1.428  
25 0.882 0.901 0.892 0.783  
12.5 0.551 0.575 0.563 0.454  
6.25 0.341 0.351 0.346 0.237  
3.12 0.245 0.245 0.245 0.136  
0 0.112 0.105 0.109    
References
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Function Endothelins are endothelium-derived vasoconstrictor peptides.
Involvement in disease Question mark ears, isolated (QME); Auriculocondylar syndrome 3 (ARCND3)
Subcellular Location Secreted
Protein Families Endothelin/sarafotoxin family
Tissue Specificity Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Database Links

HGNC: 3176

OMIM: 131240

KEGG: hsa:1906

STRING: 9606.ENSP00000368683

UniGene: Hs.511899

Pathway HIF-1 signaling pathway
Melanogenesis
TNF signaling pathway
AGE-RAGE signaling pathway in diabetic complications

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