Recombinant Human Endothelin-1(EDN1) (Active)

Code CSB-RP063044h
Size US$1726Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/ml can bind human EDN1 with a linear range of 6.25-25.00 μg/ml.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Activity Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/ml can bind human EDN1 with a linear range of 6.25-25.00 μg/ml.
Target Names EDN1
Uniprot No. P05305
Research Area Signal Transduction
Alternative Names ARCND3; Big endothelin-1; EDN1; EDN1_HUMAN; ET-1; ET1; HDLCQ7; PPET1; Preproendothelin 1; Preproendothelin-1; QME
Species Homo sapiens (Human)
Source E.coli
Expression Region 53-73aa
Target Protein Sequence CSCSSLMDKECVYFCHLDIIW
Mol. Weight 29.5kDa
Protein Length partial
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions
  1. The present study demonstrated that the circulating bigET-1 was valuable in the assessment of the severity of CAC. PMID: 29562761
  2. Unfavorable genotype of polymorphic variant of candidate gene participating in endothelial dysfunction EDN1 (Lys198Asn) was associated with changes in levels of their active substances in individuals exposed to mercury. PMID: 30351652
  3. Endothelin-1, nitric oxide, and serotonin are related to blood pressure in adolescent males. PMID: 30271160
  4. this study provides that infants with genotype GT eNOS 894G > T have 3.4-fold higher risk of developing of IVH if they are born before 28 + 6 weeks of gestation. PMID: 28211916
  5. Preeclamptic plasma stimulates the expression of miRNAs in HUVECs, leading to a decrease in endothelin-1 levels. PMID: 29674204
  6. According to the results of the present study, the culture solution with HUVECs affected the differentiation ofdental pulp stem cells (DPSCs). In addition, ET1 may promote the odontoblastic differentiation of DPSCs. PMID: 29845193
  7. EZH2, ET-1, and CD34 may act as biomarkers of aggressive cervical squamous cell carcinoma PMID: 29630085
  8. The plasma D-dimer and ET-1 levels provided a novel marker for treatment selection for the T-segment elevation acute myocardial infarction patients with a type 2 diabetes mellitus history. PMID: 29806659
  9. EDN1 rs5370 polymorphism was associated with large artery stroke development. PMID: 29849817
  10. Endothelin-1 is a regulator of Mena expression in the serous ovarian cancer.Endothelin-1 role in the invadopodian function. PMID: 29439204
  11. Studied effects of dexamethasone on gene expression regulation of sirtuin 1 (SIRT1), interleukin 6 (IL6), and endothelin 1 (EDN1) in gingival derived aging stem cells. Dexamethasone downregulated expression of SIRT1 and IL6 but upregulated EDN1 in stem cells. PMID: 29302812
  12. Baseline big ET-1>0.56pmol/L was independently associated with higher all-cause mortality and HFH among CRT recipients. PMID: 29913121
  13. this study provides the first evidence that a 2-SNP haplotype of the ET-1 gene is associated with increased risk of hormone refractory prostate cancer PMID: 29441862
  14. During exercise, we noted a rapid and significant reduction in venous ET-1 levels in obese subjects but no significant change in lean subjects. PMID: 28977804
  15. Serum levels of miR-31 and endothelin-1are elevated in psoriasis vulgaris and are suggested to play significant roles in the pathophysiology of psoriasis. PMID: 28937251
  16. Plasma big ET-1 level is a valuable independent predictor of ST in patients with coronary stents, especially in the acute coronary syndrome population. PMID: 28942357
  17. Although our study revealed higher serum ET-1 and lower serum alpha-Klotho levels in systemic sclerosis patients compared to healthy controls, there were not any significant correlations between their serum levels with severity of organ involvement. PMID: 28831601
  18. plasma EDN1 levels were significantly increased in Korean patients with fibromyalgia syndrome (FMS) compared with those in healthy controls. EDN1 SNP was revealed to be associated with susceptibility to FMS. PMID: 28901422
  19. miR-200c regulates endothelin-1 induced pulmonary artery smooth muscle cells abnormal proliferation and apoptosis. PMID: 29044995
  20. Increased ET-1 levels after 3 years were positively associated pulse pressure, suggesting subclinical haemodynamic changes with potential premature onset of cardiovascular disease in the black South Africans. PMID: 28726796
  21. The presence of the rs2070699 T allele of EDN1 increased the risk of persistent pulmonary hypertension of the newborn in neonates with respiratory distress. PMID: 27425626
  22. Studied the associations of Protein Z (PROZ G79A) and Endothelin-1 (EDN1 G5665T) polymorphisms with the clinical features of sickle cell disease (SCD) in a cohort of Egyptian children with SCD. PMID: 28548215
  23. Endothelin-1 rs4714384 is an important biomarker of cardiovascular events in end stage renal disease patients. PMID: 28882114
  24. We provided some ways of distinguishing Retinal vein occlusion (RVO)patients in practice and introduced the vasoactivity of the vein itself and the possible involvement of vasoconstrictors such as ET-1 for the pathogenesis of RVO through our clinical studies, some animal experiments, and recent literatures. PMID: 28904960
  25. High levels of plasma endothelin-1 predicted prognosis for patients with hypertrophic cardiomyopathy. PMID: 28587741
  26. highly expressed in the dermal microvascular endothelial cells of hypertrophic scars and keloids PMID: 27892645
  27. ET-1 promoted the proliferation, migration and secretion of extracellular matrix molecules through the protein kinase B (Akt) and extracellular signal-regulated kinase (Erk) signaling pathways in RPE cells in vitro. Thus, ET-1 may play a vital role in the development of PVR. PMID: 28634054
  28. Increased baseline values of ET-1 (and BNP) after acute myocardial infarction, were independently predictive of future cardiovascular events. PMID: 28284500
  29. The EDN1 gene polymorphism may play a significant role in predicting individual's susceptibility toward venous thromboembolism and its clinical progression PMID: 27481876
  30. Epiretinal membrane formation in proliferative diabetic retinopathy involves higher tissue levels of endothelin-1 and fibroblastic activity PMID: 29351334
  31. this study did not find any significant differences of ET-1 level between obstetric antiphospholipid syndrome and thrombotic antiphospholipid syndrome PMID: 27267037
  32. Polymorphic variants of endothelin EDN (K198N) and endothelin receptor type A genes EDN RA (C1222T, C70G, G231A) affected ET plasma concentrations. There was no association between the plasma endothelin levels and the risk factors for normal tension glaucoma. PMID: 27777505
  33. high glucose and free fatty acids reduced NO and increased ET-1 levels induced by acetylcholine through the modulation of intracellular calcium concentrations and endothelial NO synthase phosphorylation, events also reverted by hydroxytyrosol and polyphenol extract. PMID: 25460732
  34. In both the acute and remission phase of thrombotic thrombocytopenic purpura we found increased CT-proET-1 and VWF levels, while Factor H levels decreased compared with healthy controls. In remission, however, the elevated CT-proET-1 levels showed a 22 % decrease when compared with the acute phase. PMID: 26763086
  35. ET-1 increased NSCLC clonal growth, whereas BQ123, ZD4054, lapatinib or gefitinib inhibited proliferation. The results indicate that ET-1 may regulate NSCLC cellular proliferation in an EGFR- and HER2-dependent manner PMID: 28153500
  36. Data indicate that patients with systemic sclerosis (SSc) displayed higher serum levels of VEGF, endothelin-1 and s-Fractalkine, and that the s-Fractalkine levels positively correlated with CD34(+)CD45(-) endothelial progenitor cells (EPC) numbers. PMID: 28320472
  37. Endothelin-1 and its receptors on haemorrhoidal tissue: a potential site for therapeutic intervention PMID: 28095606
  38. we could provide evidence that established stress-related biomarkers ET-1, MCP-1, CGA were differentially regulated among patients with AF compared to healthy controls. PMID: 28886122
  39. Two END1 polymorphisms do not appear to affect the development of hypertension in the Chinese population. PMID: 28692114
  40. TWEAK may stimulate endothelial ET-1 synthesis, through the up-regulation of ECE-1. PMID: 28025386
  41. ET-1 may be a unique predictor of heart failure prognosis PMID: 28398455
  42. The EDNRA SNP rs5335 was independently associated with aneurysms >/=7 mm in size at the time of rupture. Patients with cerebral aneurysms also carrying the G allele of EDNRA SNP rs5335 may develop larger aneurysms before rupture. PMID: 28323184
  43. c-Src tyrosine kinase mediates high glucose-induced endothelin-1 expression PMID: 27102411
  44. This review takes us through the drug discovery journey that led to the discovery, development, and registration of macitentan. [review] PMID: 27534881
  45. Endothelin contributes to the rise in SBP in response to acute hypoxia in patients with severely untreated OSA. PMID: 27906839
  46. Results show a correlation between ET-1 and an impaired liver function, quantified by the maximal liver function capacity test, in critically ill patients. The findings approve the close interaction between TNF-alpha and ET-1 in sepsis. PMID: 28542386
  47. The mean serum levels of endothelin-1 in Egyptian acute myocardial infarction patients was significantly higher than controls. PMID: 27761928
  48. The ratio of serum levels of adiponectin to endothelin strongly correlates with severity of coronary artery disease. PMID: 28781261
  49. Higher levels of circulating endothelin-1 on admission independently predicts subsequent hospital major cardiac events in patients presenting with STEMI. PMID: 27372047
  50. ET-1 is upregulated in giant cell arteritis (GCA) lesions and, by promoting VSMC migration towards the intimal layer, may contribute to intimal hyperplasia and vascular occlusion in GCA. PMID: 28606962
  51. Our findings advance our knowledge pertaining to the peripheral control underpinning the regulation of cutaneous blood flow and sweating and infer that ET-1 may attenuate the heat loss responses of cutaneous blood flow, but not sweating PMID: 26846374
  52. endothelin-1 correlated positively with glutathione reductase activity and negatively with antihypertension medication and tended to correlate with glutathione reductase-to-glutathione peroxidase ratio in black men. In white men, endothelin-1 correlated positively with ROS and negatively with glutathione peroxidase activity. In black women, endothelin-1 correlated negatively with total glutathione. PMID: 27682654
  53. CRISPR-edited stem cell-derived endothelial cells demonstrate rs9349379, a common SNP in the 3 intron of the PHACTR1 gene, regulates expression of endothelin 1 (EDN1), a gene located 600 kb upstream of PHACTR1. The known physiologic effects of EDN1 on the vasculature may explain the pattern of risk for the five associated vascular diseases. PMID: 28753427
  54. demonstrated the fundamental role of NF-kB signalling pathway in promoting and maintaining ET-1 expression in CLL cells, both in basal conditions and after CD40 activation PMID: 28092772
  55. an important role of ET-1 and endothelial function in the regulation of kidney function PMID: 28222015
  56. activation of ETAR by ET-1 promotes HCC cell migration and invasion via activating ERK1/2 and AKT signaling pathways and upregulating MMP-3 expression. Thus, ETAR may play an important role in the progress of HCC. PMID: 26501871
  57. cellular and molecular mechanisms of ET-1 action in periodontitis PMID: 28030574
  58. Alternatively activated (M2) macrophage phenotype is inducible by endothelin-1 in cultured macrophages. PMID: 27846260
  59. These findings indicate that TT is a practical and effective strategy to reduce BP and circulating ET-1 concentration and enhance leg blood flow in patients with PAD. PMID: 27335279
  60. reduced lung levels of PPARgamma and increased levels of microRNA-27a (miR-27a), v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS1), endothelin-1 (ET-1), and markers of endothelial dysfunction (platelet/endothelial cell adhesion molecule 1 and E selectin). PMID: 27612006
  61. We show that in young adults, Rho kinase is an important mediator of the endothelin-1-mediated attenuation of endothelium-dependent and -independent cutaneous vasodilation, and that endothelin-1 does not increase sweating. PMID: 27881399
  62. ET-1 levels were significantly elevated in patients with chronic stable heart failure with preserved ejection fraction. PMID: 27810862
  63. Differences were found in the neonatal and maternal expression of leptin, adiponectin, adropin, and endothelin-1 in intrauterine growth restricted mothers and neonates compared with a control group. PMID: 26352058
  64. Increased circulating Edn1 and expression of Ednra in endothelial cells are characteristic of diabetic kidney disease. PMID: 27899487
  65. Big ET-1 was strongly and independently associated with coronary artery ectasia by multivariate analysis PMID: 27885846
  66. Leptin to insulin and adiponectin to endothelin ratios are novel derived biomarkers useful for noninvasive diagnostics of initial stages of coronary lesions in patients with coronary artery disease. PMID: 27300270
  67. The conjunct use of Big-ET-1 and NT-proBNP improves the risk stratification of patients with intermediate to high risk of cardiovascular (CV) disease death and congestive heart failure. Big-ET-1improves risk stratification in patients referred for coronary angiography. PMID: 27788598
  68. The paternal rs5370 polymorphism decreases the risk for preeclampsia and is not associated with placental expression of the EDN1 gene. PMID: 27939477
  69. high percentages of HIF-1alpha (100%), VEGF (89.5%), iNOS (78.9%), and ET-1 (84.2%) expressions were observed in congenital heart disease autopsy cases and this was found to be significant. PMID: 27450023
  70. The level of ET-1 and eNOS plasma was not significantly different between the CMS patients and healthy controls PMID: 27485004
  71. Coronary occlusion patients in the poor coronary collateral circulation ( CCC ) group had a higher ET1 level than those in the good CCC group (0.59 +/- 0.48 vs. 0.39 +/- 0.32 pmol/L, p < 0.001), and the ET1 values increased with the descent of the Rentrop grades (p for trend < 0.001). PMID: 27105310
  72. Activin A and preeclamptic serum upregulate ET-1, ICAM-1, and VCAM-1 in human umbilical vein endothelial cells, consistent with activin A contributing to the pathophysiology of preeclampsia. PMID: 27155341
  73. ET1 gene and ECE-1 expression levels in human umbilical vein endothelial cells and ET-1 expression levels in the cell culture supernatants increased significantly in some experimental groups compared with those in the control group. PMID: 27941335
  74. Serum and synovial fluid endothelin-1 concentrations are correlated with the development and progression of knee osteoarthritis. PMID: 26917562
  75. This study demonstrated the pathogenesis mechanism during the development of dementia after ischemic stroke by investigating the relationship between miR-125a and its target ET-1. PMID: 27106952
  76. Urinary TGF-beta1 and ET-1 levels were associated with AGT level, which likely reflects an early interplay between tissue remodeling and RAAS in obesity-related kidney injury. PMID: 26482255
  77. High circulating endothelin-1 levels are associated with the development of impaired glucose tolerance and type 2 diabetes in women. PMID: 26972958
  78. Serum endothelin-1 was elevated in preeclampsia and correlated with severity of illness. PMID: 26823880
  79. There was no significant difference in serum EDN1 between patients with vasovagal syncope, epilepsy, and controls. PMID: 26641207
  80. Therefore, despite a clear age-specific role in the regulation of vascular tone, endogenous ET-1, acting through endothelin type A receptors, does not account for the age-related reduction in mean shear rate in the common femoral artery. PMID: 26599223
  81. In patients with autosomal dominant polycystic kidney disease, urinary ET-1 was inversely associated with eGFR and positively correlated with total kidney volume. PMID: 26923419
  82. Congenital heart disease complicated with pulmonary artery hypertension is associated with increased circulating endothelial cell counts and ET-1 production. PMID: 26617785
  83. demonstration that pericardial fluid (PF) of cardiac patients elicit substantial arterial constrictions, which is mediated primarily by ET-1; interfering with the vasoconstrictor action of PF could be a potential therapeutic target to improve coronary blood flow in cardiac patients PMID: 26322806
  84. EDN1 as a key factor towards the generation and clonal derivation of ISL1(+) vascular intermediates. PMID: 26952167
  85. Data show that plasma levels of endothelial microparticle (EMP) and endothelin-1 (ET-1 are significantly higher in intermediate )lesion group, while their nitric oxide (NO) and nitric oxide synthase 3 (eNOS) are lower. PMID: 26554436
  86. we show that fenofibrate, a PPAR-alpha agonist, increased the expression of miR-301a/miR-454 and SKA2 in human microvascular endothelial cell line (HMEC) cells; the former were responsible for reduced expression of ET-1 and PAI-1 PMID: 26460070
  87. Peripheral endothelin-1 and transforming growth factor-beta1 levels, which strongly correlate with their hepatic levels, reflect the stage of portal hypertension and liver insufficiency in cirrhosis. PMID: 26144293
  88. increased serum level is an independent predictor of digital ulcer in systemic sclerosis PMID: 26142066
  89. excessive ET-1 production in the setting of HIV infection impairs pulmonary endothelial function and contributes to the development of pulmonary arterial hypertension . PMID: 26752193
  90. The data demonstrated that the plasma big endothelin 1 level was a valuable independent predictor for coronary artery calcification. PMID: 26565974
  91. Circulating endothelin-1 levels may predict coronary heart disease in women. PMID: 26573599
  92. A(8002)G intronic polymorphism of pre-pro-endothelin-1 gene is associated with elevated endothelin-1 levels among Tunisian coronary patients. PMID: 26573609
  93. Endothelin-1 level and intima-media thickness were higher in chronic kidney disease patients without any correlation between endothelin-1 and peripheral arteries' intima-media thickness. PMID: 25245046
  94. these data from a population-based study in outpatients with cardiovascular risk factors revealed that circulating C-terminal ET-1 precursor fragment levels are negatively associated with anxiety. PMID: 26322793
  95. For the diagnosis of HELLP syndrome, increased levels of ET-1 [Endothelin-1], M30, and Angs-1 and -2 appear as promising biomarkers after determination of their standardized threshold levels PMID: 26138305
  96. Meta-analysis: statin therapy significantly reduces circulating ET-1 concentrations, regardless of treatment duration or dose of statins. PMID: 26074317
  97. We aimed to provide a theoretical basis for improving patient prognosis through analyzing expression of plasma brain natriuretic peptide (BNP), endothelin-1 (ET 1), and matrix metalloproteinase 9 (MMP-9). PMID: 26190179
  98. PPARgamma regulates miR-98 to modulate ET-1 expression and pulmonary endothelial cell proliferation in pulmonary hypertension. PMID: 26098770
  99. An increase of lipid peroxide and endothelin-1 associated with nuclear factor-kappaB activation plays an important role in the development of diabetic angiopathies. PMID: 26710051
  100. Endothelin-1 seems to predispose patients undergoing cardiac surgery to develop nonocclusive mesenteric ischemia. PMID: 25623906

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Involvement in disease Question mark ears, isolated (QME); Auriculocondylar syndrome 3 (ARCND3)
Subcellular Location Secreted
Protein Families Endothelin/sarafotoxin family
Tissue Specificity Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Database Links

HGNC: 3176

OMIM: 131240

KEGG: hsa:1906

STRING: 9606.ENSP00000368683

UniGene: Hs.511899

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