GC Content Calculator for Nucleic Acid Sequences

Quickly calculate the percentage of Guanine (G) and Cytosine (C) in DNA or RNA sequences

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Sequence can include the following characters:

A (Adenine) T (Thymine) G (Guanine) C (Cytosine) U (Uracil)

What is GC Content?

GC content refers to the percentage of Guanine (G) and Cytosine (C) in a DNA or RNA sequence. In molecular biology, GC content is an important parameter that affects the stability and melting point of DNA.

Biological Significance

DNA sequences with high GC content are generally more stable because there are three hydrogen bonds between G and C, while only two between A and T.

Applications

GC content is widely used in PCR primer design, genomic analysis, and phylogenetic studies.

Reference Range

Genomic GC content varies widely among different species, typically ranging from 30% to 70%.

How to Use this GC Content Calculator?

For a quick walkthrough, watch the demonstration video below. It will guide you through using this calculator effectively and interpreting the results with ease.

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